Multi-Omics Analysis of the Effect of cAMP on Actinorhodin Production in Streptomyces coelicolor

Nitta, Katsuaki; Carratore, Francesco Del; Breitling, Rainer; Takano, Eriko; Putri, Sastia Prama; Fukusaki, Eiichiro

doi:10.3389/fbioe.2020.595552

Cited by 6 publications

(4 citation statements)

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“…2b ). In a recent study, transcriptome sequencing (RNAseq) data were collected for the S. coelicolor M1146 strain cultivated using phosphate-limited fully defined medium ( 21 ). After excluding the rRNA genes from the analysis, the homologue of ssrA (SCOs02) was always the most expressed gene, and the homologue of ffs (SCOs03) was found in the top 30 most highly expressed genes in all experimental conditions considered.…”

Section: Resultsmentioning

confidence: 99%

Multi-omics Study of Planobispora rosea, Producer of the Thiopeptide Antibiotic GE2270A

Carratore

Iorio²,

Pérez-Bonilla

et al. 2021

mSystems

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Section: Resultsmentioning

confidence: 99%

Multi-omics Study of Planobispora rosea, Producer of the Thiopeptide Antibiotic GE2270A

Carratore

Iorio²,

Pérez-Bonilla

et al. 2021

mSystems

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“…In pleiotropically defective mutants, mutations in a diversity of genes have been identified, for example, mutations in genes encoding sigma factors [ 25 ], DNA-binding proteins [ 26 ], tRNAs [ 27 ], RNase III [ 28 ], protein kinases [ 10 ], methyltransferases [ 29 ], adenylate cyclase [ 30 ], or S-Adenosyl-L-methionine synthetase [ 31 ], implicating that both the morphological and physiological differentiation of Streptomycetes are affected by various factors. Moreover, some global regulatory genes can function beyond species barriers.…”

Section: Discussionmentioning

confidence: 99%

SCO6992, a Protein with β-Glucuronidase Activity, Complements a Mutation at the absR Locus and Promotes Antibiotic Biosynthesis in Streptomyces coelicolor

Jin

Choi

Tsevelkhoroloo

et al. 2021

J. Microbiol. Biotechnol.

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IntroductionStreptomycetes is a group of gram-positive and filamentous soil bacteria with a unique and complex differentiation process that includes simultaneous morphological (sporulation) and physiological (secondary metabolism) differentiation. Many scientific findings have elucidated that the two differentiation processes are genetically closely related and thus controlled concurrently by many regulatory factors. Generally, genes for secondary metabolite formation and morphological differentiation are clustered in the chromosome, and their expression is precisely controlled by a pathway-specific regulatory gene(s) within the cluster as well as by global regulatory genes [1][2][3].Several classes of mutants that simultaneously block the synthesis of more than one antibiotic constitute a good genetic evidence of a global regulatory system. Streptomyces coelicolor has been known to produce four genetically and structurally distinct antibiotics: the pigmented antibiotics actinorhodin and undecylprodigiosin and the unpigmented antibiotics methylenomycin and CDA (Ca 2+ -dependent antibiotic) [4]. According to an S. coelicolor genome sequence analysis, 18 gene clusters are expected to code exclusively for enzymes characteristic of its secondary metabolism [5]. abs (antibiotic synthesis deficient) mutants, for example, affect global regulatory genes involved only in physiological differentiation. Among them, absmutations absA -, absB -, and absCwere found to lack all four antibiotics, but retained normal sporulation. The molecular nature of the absA1/A2 gene products that can restore the bacterial ability for biosynthesizing all four antibiotics was identified as a two-component regulatory system, composed by the sensor histidine kinase AbsA1 and the aspartate kinase response regulator AbsA2 [4]. The phosphorylated AbsA2 is known to act as a global negative regulator of antibiotic biosynthesis in S.Streptomyces coelicolor is a filamentous soil bacterium producing several kinds of antibiotics. S. coelicolor abs8752 is an abs (antibiotic synthesis deficient)-type mutation at the absR locus; it is characterized by an incapacity to produce any of the four antibiotics synthesized by its parental strain J1501. A chromosomal DNA fragment from S. coelicolor J1501, capable of complementing the absphenotype of the abs8752 mutant, was cloned and analyzed. DNA sequencing revealed that two complete ORFs (SCO6992 and SCO6993) were present in opposite directions in the clone. Introduction of SCO6992 in the mutant strain resulted in a remarkable increase in the production of two pigmented antibiotics, actinorhodin and undecylprodigiosin, in S. coelicolor J1501 and abs8752. However, introduction of SCO6993 did not show any significant difference compared to the control, suggesting that SCO6992 is primarily involved in stimulating the biosynthesis of antibiotics in S. coelicolor. In silico analysis of SCO6992 (359 aa, 39.5 kDa) revealed that sequences homologous to SCO6992 were all annotated as hypothetical proteins. Although a metall...

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“… 2020 ), to understanding the effect of extracellular cAMP as a regulator of secondary metabolite production (Nitta et al . 2020 ).…”

Section: Molecular Profiling For Actinomycete Synthetic Biologymentioning

confidence: 99%

Synthetic biology approaches to actinomycete strain improvement

Breitling

Avbelj

Bilyk

et al. 2021

FEMS Microbiology Letters

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Their biochemical versatility and biotechnological importance make actinomycete bacteria attractive targets for ambitious genetic engineering using the toolkit of synthetic biology. But their complex biology also poses unique challenges. This mini review discusses some of the recent advances in synthetic biology approaches from an actinomycete perspective and presents examples of their application to the rational improvement of industrially relevant strains.

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Multi-Omics Analysis of the Effect of cAMP on Actinorhodin Production in Streptomyces coelicolor

Cited by 6 publications

References 44 publications

Multi-omics Study of Planobispora rosea, Producer of the Thiopeptide Antibiotic GE2270A

Multi-omics Study of Planobispora rosea, Producer of the Thiopeptide Antibiotic GE2270A

SCO6992, a Protein with β-Glucuronidase Activity, Complements a Mutation at the absR Locus and Promotes Antibiotic Biosynthesis in Streptomyces coelicolor

Synthetic biology approaches to actinomycete strain improvement

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