Multi-omics approach for identification of molecular alterations of QiShenYiQi dripping pills in heart failure with preserved ejection fraction

Huang, Yu‐Ting; Zhang, Kai; Wang, Xiao; Guo, Kaimin; Liu, Xiaoqiang; Chen, Feng; Du, Ruijiao; Li, Sheng; Li, Lan; Zhang, Yang; Zhuo, Danping; Wang, Bingkai; Wang, Wenjia; Hu, Yunhui; Jiang, Miaomiao; Fan, Guanwei

doi:10.1016/j.jep.2023.116673

Cited by 6 publications

(2 citation statements)

References 45 publications

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“…Besides, the adjuvant is Jiangxiang. Huang et al (2023) also found that Qishen Yiqi dripping pills have an effect on smooth circulation and stopping pains, which could also promote blood stasis and stop bleeding. Based on modern pharmacological research, Qishen Yiqi dripping pills could adjust vitality and nourish the blood, stimulating pulse, and alleviating pain, which could be used to prevent myocardial injury and enhance the production of myocardial cells (Wu et al, 2023).…”

Section: Introductionmentioning

confidence: 95%

“…Qishen Yiqi dripping pills are the dripping pills dosage form prepared by Modern Pharmaceutical Technology, including four Traditional Chinese Medicine (TCM), namely, Huangqi, Danshen, Sanqi, and Jiangxiang. Huang et al (2023) found that the sovereign medicine is Huangqi, and the ministerial drug adjuvant drug is Danshen and Sanqi in the preparation.…”

Section: Introductionmentioning

confidence: 99%

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Determination of Four Chemical Components in Qishen Yiqi Dripping Pills by UPLC-MS/MS Method

Lili,

Honglian,

Yanxia

et al. 2023

Pharmacognosy Magazine

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Background In this study, the ultrahigh performance liquid chromatography mass spectrometry/mass spectrometry (UPLC-MS/MS) method was established to test the contents of protocatechuic acid (C7H6O4), salvianolic acid B (C36H30O16), rosmarinic acid (C19H14O3), and cryptotanshinone (C19H20O3) in Qishen Yiqi dripping pills. Materials and Methods Methanol was chosen as the solvent according to a certain material–liquid ratio as a solution, and the ultrasonic extraction method was used to prepare the sample solution. UPLC-MS/MS was used to complete a quantitative analysis of the contents of Qishen Yiqi dripping pills. Then, the chromatographic conditions were shown as follows. The stationary phase was Agilent ZORBAX SB-C18 (2.1 mm × 100 mm, 1.8 µm), and the mobile phase was acetonitrile–0.1% formic acid water. The column temperature was 40℃, the injection volume was 3 µL, and the flow rate was 0.2 mL⋅min–1. Finally, the determination method was built to test four chemical constituents under the multiple reaction monitoring (MRM) analysis mode. Results The retention times of protocatechuic acid, salvianolic acid B, rosmarinic acid, and cryptotanshinone were 1.26, 1.32, 1.35, and 6.76 min, respectively. In addition, the concentration ranges of four compounds were 0.25–2.0 µg⋅mL–1, 10.0–50.0 µg⋅mL–1, 5.0–50.0 µg⋅mL–1, and 1.0–10.0 µg⋅mL–1, respectively. And the standard curve showed a good linear relationship in a reasonable range ( r2 ≥ 0.9950). The average recovery rate was from 96% to 124%. The contents of protocatechuic acid, salvianolic acid B, rosmarinic acid, and cryptotanshinone were 0.12%–0.022%, 17%–8.4%, and 6.5%–8.6% in seven batches of Qishen Yiqi dripping pills, which were in the range of 23%–31%. The instrument ran stably, and the method had good accuracy and repeatability (relative standard deviation [RSD] ≤ 3%). Additionally, the test result was stable within 10 h. Conclusion The contents of the four constituents were different among different batches. The quantitative method was established to determine the four chemical components in Qishen Yiqi dripping pills, which could provide a reference for quality evaluation in vivo.

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Section: Introductionmentioning

confidence: 95%

Section: Introductionmentioning

confidence: 99%