Multi-Omics Research Trends in Sepsis: A Bibliometric, Comparative Analysis Between the United States, the European Union 28 Member States, and China

Evangelatos, Nikolaos; Satyamoorthy, Kapaettu; Levidou, Georgia; Bauer, Pia; Brand, Helmut; Kouskouti, Christina; Lehrach, Hans; Brand, Angela

doi:10.1089/omi.2017.0192

Cited by 12 publications

(7 citation statements)

References 42 publications

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“…Immunocompromised conditions have been found to affect the outcome of sepsis substantially; however, the underlying mechanism remains elusive due to the complexity and high heterogeneity of sepsis (23,24). The RNA-Seq has been increasingly used to address complex diseases, namely, sepsis, which disrupts the pathophysiological homeostasis result from complex, numerous, intertwined, and subcellular biological events (25). Several studies have used RNA-Seq to identify the subendotype in sepsis, and the identification of subendotype should be essential for individualised medicine in patients with sepsis (26).…”

Section: Discussionmentioning

confidence: 99%

Using RNA-Seq to Investigate Immune-Metabolism Features in Immunocompromised Patients With Sepsis

et al. 2021

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Objective: Sepsis is life threatening and leads to complex inflammation in patients with immunocompromised conditions, such as cancer, and receiving immunosuppressants for autoimmune diseases and organ transplant recipients. Increasing evidence has shown that RNA-Sequencing (RNA-Seq) can be used to define subendotype in patients with sepsis; therefore, we aim to use RNA-Seq to identify transcriptomic features among immunocompromised patients with sepsis.Methods: We enrolled patients who were admitted to medical intensive care units (ICUs) for sepsis at a tertiary referral centre in central Taiwan. Whole blood on day-1 and day-8 was obtained for RNA-Seq. We used Gene Set Enrichment Analysis (GSEA) to identify the enriched pathway of day-8/day-1 differentially expressed genes and MiXCR to determine the diversity of T cell repertoire.Results: A total of 18 immunocompromised subjects with sepsis and 18 sequential organ failure assessment (SOFA) score-matched immunocompetent control subjects were enrolled. The ventilator-day, ICU-stay, and hospital-day were similar between the two groups, whereas the hospital mortality was higher in immunocompromised patients than those in immunocompetent patients (50.0 vs. 5.6%, p < 0.01). We found that the top day-8/day-1 upregulated genes in the immunocompetent group were mainly innate immunity and inflammation relevant genes, namely, PRSS33, HDC, ALOX15, FCER1A, and OLR1, whereas a blunted day-8/day-1 dynamic transcriptome was found among immunocompromised patients with septic. Functional pathway analyses of day-8/day-1 differentially expressed genes identified the upregulated functional biogenesis and T cell-associated pathways in immunocompetent patients recovered from sepsis, whereas merely downregulated metabolism-associated pathways were found in immunocompromised patients with septic. Moreover, we used MiXCR to identify a higher diversity of T cell receptor (TCR) in immunocompetent patients both on day-1 and on day-8 than those in immunocompromised patients.Conclusions: Using RNA-Seq, we found compromised T cell function, altered metabolic signalling, and decreased T cell diversity among immunocompromised patients with septic, and more mechanistic studies are warranted to elucidate the underlying mechanism.

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Section: Discussionmentioning

confidence: 99%

Using RNA-Seq to Investigate Immune-Metabolism Features in Immunocompromised Patients With Sepsis

et al. 2021

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“…Furthermore, fiberoptic bronchoscopy and bronchoalveolar lavage (FOB/BAL) are commonly used for diagnosis, but may cause further respiratory deterioration in patients with hypoxemia [4]. The development of non-invasive diagnostic tests with high sensitivity and specificity (e.g., on blood, plasma, sputum, urine, or nasal swabs) has obviated the need for FOB/BAL in some patients [5,6]. The utility of these non-invasive tests is being evaluated, and will hopefully provide clinicians with additional tools in the diagnosis of these complex patients.…”

Section: Introductionmentioning

confidence: 99%

Diagnosis of severe respiratory infections in immunocompromised patients

et al. 2020

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An increasing number of critically ill patients are immunocompromised. Acute hypoxemic respiratory failure (ARF), chiefly due to pulmonary infection, is the leading reason for ICU admission. Identifying the cause of ARF increases the chances of survival, but may be extremely challenging, as the underlying disease, treatments, and infection combine to create complex clinical pictures. In addition, there may be more than one infectious agent, and the pulmonary manifestations may be related to both infectious and non-infectious insults. Clinically or microbiologically documented bacterial pneumonia accounts for one-third of cases of ARF in immunocompromised patients. Early antibiotic therapy is recommended but decreases the chances of identifying the causative organism(s) to about 50%. Viruses are the second most common cause of severe respiratory infections. Positive tests for a virus in respiratory samples do not necessarily indicate a role for the virus in the current acute illness. Invasive fungal infections (Aspergillus, Mucorales, and Pneumocystis jirovecii) account for about 15% of severe respiratory infections, whereas parasites rarely cause severe acute infections in immunocompromised patients. This review focuses on the diagnosis of severe respiratory infections in immunocompromised patients. Special attention is given to newly validated diagnostic tests designed to be used on non-invasive samples or bronchoalveolar lavage fluid and capable of increasing the likelihood of an early etiological diagnosis.

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“…Modern omics technologies enable identification of multiple pathways potentially involved in the onset and progression of MOD in human septic patients (Evangelatos et al 2018, Langley et al 2013, Liu et al 2014). Strategy of "reverse translation" based on identification of these pathways in humans and their subsequent verification on appropriate animal model would help to avoid analysis and fruitless translation of phenomena that are strictly species-specific (Efron et al 2015).…”

Section: Perspectivesmentioning

confidence: 99%

“…Sepsis, life-threatening organ dysfunction caused by a dysregulated host response to infection, represents one of the most serious public health issues of the modern time. In the United States it affects more than 750,000 patients per year ; its incidence is similar in Europe and probably even higher in low-income countries (Fleischmann et al 2016). Mortality rate ranges between 30-70 % in relation to severity of the disease, particularly to the number of dysfunctional organs (da Silva et al 2008).…”

Section: Introductionmentioning

confidence: 99%

Variability of Mitochondrial Respiration in Relation to Sepsis-Induced Multiple Organ Dysfunction

Kohoutová¹,

Dejmek²,

Tůma³

et al. 2018

Physiol Res

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Ample experimental evidence suggests that sepsis could interfere with any mitochondrial function; however, the true role of mitochondrial dysfunction in the pathogenesis of sepsis-induced multiple organ dysfunction is still a matter of controversy. This review is primarily focused on mitochondrial oxygen consumption in various animal models of sepsis in relation to human disease and potential sources of variability in experimental results documenting decrease, increase or no change in mitochondrial respiration in various organs and species. To date, at least three possible explanations of sepsis-associated dysfunction of the mitochondrial respiratory system and consequently impaired energy production have been suggested: 1. Mitochondrial dysfunction is secondary to tissue hypoxia. 2. Mitochondria are challenged by various toxins or mediators of inflammation that impair oxygen utilization (cytopathic hypoxia). 3. Compromised mitochondrial respiration could be an active measure of survival strategy resembling stunning or hibernation. To reveal the true role of mitochondria in sepsis, sources of variability of experimental results based on animal species, models of sepsis, organs studied, or analytical approaches should be identified and minimized by the use of appropriate experimental models resembling human sepsis, wider use of larger animal species in preclinical studies, more detailed mapping of interspecies differences and organ-specific features of oxygen utilization in addition to use of complex and standardized protocols evaluating mitochondrial respiration.

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Multi-Omics Research Trends in Sepsis: A Bibliometric, Comparative Analysis Between the United States, the European Union 28 Member States, and China

Cited by 12 publications

References 42 publications

Using RNA-Seq to Investigate Immune-Metabolism Features in Immunocompromised Patients With Sepsis

Using RNA-Seq to Investigate Immune-Metabolism Features in Immunocompromised Patients With Sepsis

Diagnosis of severe respiratory infections in immunocompromised patients

Variability of Mitochondrial Respiration in Relation to Sepsis-Induced Multiple Organ Dysfunction

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