Multi-omics reveals the key and specific miRNA-mRNA modules underlying salt tolerance in wild emmer wheat (Triticum dicoccoides L.)

Yang, Guang; Pan, Wenqiu; Cao, Rui; Guo, Qifan; Cheng, Yue; Zhao, Qinlong; Cui, Licao; Nie, Xiaojun

doi:10.1186/s12864-022-08945-3

Cited by 5 publications

(3 citation statements)

References 61 publications

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“…Specifically, tae−miR408 has been identified as playing a crucial role in regulating wheat responses to Pi starvation and salt stress [ 17 ]. At the same time, whole genome screening and the identification of different varieties such as bread wheat and diploid wild wheat were conducted, and many different types of miRNAs were identified [ 25 , 45 , 46 ].…”

Section: Discussionmentioning

confidence: 99%

Comparative Analysis of miRNA Expression Profiles under Salt Stress in Wheat

Qiao

Jiao

Wang

et al. 2023

Genes

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Salt stress is one of the important environmental factors that inhibit the normal growth and development of plants. Plants have evolved various mechanisms, including signal transduction regulation, physiological regulation, and gene transcription regulation, to adapt to environmental stress. MicroRNAs (miRNAs) play a role in regulating mRNA expression. Nevertheless, miRNAs related to salt stress are rarely reported in bread wheat (Triticum aestivum L.). In this study, using high−throughput sequencing, we analyzed the miRNA expression profile of wheat under salt stress. We identified 360 conserved and 859 novel miRNAs, of which 49 showed considerable changes in transcription levels after salt treatment. Among them, 25 were dramatically upregulated and 24 were downregulated. Using real−time quantitative PCR, we detected significant changes in the relative expression of miRNAs, and the results showed the same trend as the sequencing data. In the salt−treated group, miR109 had a higher expression level, while miR60 and miR202 had lower expression levels. Furthermore, 21 miRNAs with significant changes were selected from the differentially expressed miRNAs, and 1023 candidate target genes were obtained through the prediction of the website psRNATarget. Gene ontology (GO) analysis of the candidate target genes showed that the expressed miRNA may be involved in the response to biological processes, molecular functions, and cellular components. In addition, the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis confirmed their important functions in RNA degradation, metabolic pathways, synthesis pathways, peroxisome, environmental adaptation, global and overview maps, and stress adaptation and the MAPK signal pathway. These findings provide a basis for further exploring the function of miRNA in wheat salt tolerance.

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Section: Discussionmentioning

confidence: 99%

Comparative Analysis of miRNA Expression Profiles under Salt Stress in Wheat

Qiao

Jiao

Wang

et al. 2023

Genes

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“…For common wheat, which has an enormous and complex hexaploid genome, transcriptome sequencing is a rapid and effective method for mining candidate genes [ 4 , 5 , 6 ], especially in studies involving a stress response. Based on RNA-sequencing (RNA-Seq) data, many important biological or abiotic stress response genes have been further identified, such as the leaf rust-resistance gene Lr47 [ 7 ] and the cold tolerance gene TaSnRK1α [ 8 ].…”

Section: Introductionmentioning

confidence: 99%

“…For common wheat, which has an enormous and complex hexaploid genome, transcriptome sequencing is a rapid and effective method for mining candidate genes [4][5][6],…”

Section: Introductionmentioning

confidence: 99%

RNA-Seq-Based WGCNA and Association Analysis Reveal the Key Regulatory Module and Genes Responding to Salt Stress in Wheat Roots

Chen,

Zhang,

Liu

et al. 2024

Plants

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Soil salinization is the main abiotic stressor faced by crops. An improved understanding of the transcriptional response to salt stress in roots, the organ directly exposed to a high salinity environment, can inform breeding strategies to enhance tolerance and increase crop yield. Here, RNA-sequencing was performed on the roots of salt-tolerant wheat breeding line CH7034 at 0, 1, 6, 24, and 48 h after NaCl treatment. Based on transcriptome data, a weighted gene co-expression network analysis (WGCNA) was constructed, and five gene co-expression modules were obtained, of which the blue module was correlated with the time course of salt stress at 1 and 48 h. Two GO terms containing 249 differentially expressed genes (DEGs) related to osmotic stress response and salt-stress response were enriched in the blue module. These DEGs were subsequently used for association analysis with a set of wheat germplasm resources, and the results showed that four genes, namely a Walls Are Thin 1-related gene (TaWAT), an aquaporin gene (TaAQP), a glutathione S-transfer gene (TaGST), and a zinc finger gene (TaZFP), were associated with the root salt-tolerance phenotype. Using the four candidate genes as hub genes, a co-expression network was constructed with another 20 DEGs with edge weights greater than 0.6. The network showed that TaWAT and TaAQP were mainly co-expressed with fifteen interacting DEGs 1 h after salt treatment, while TaGST and TaZFP were mainly co-expressed with five interacting DEGs 48 h after salt treatment. This study provides key modules and candidate genes for understanding the salt-stress response mechanism in wheat roots.

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