Multi-omics study and ncRNA regulation of anti-BmNPV in silkworms, Bombyx mori: an update

Fan, Yi-Xuan; Andoh, Vivian; Chen, Liang

doi:10.3389/fmicb.2023.1123448

Cited by 5 publications

(3 citation statements)

References 110 publications

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“…Following the A. apis infection, 386, 351, and 272 DElncRNAs were detected in the 4-, 5-, and 6-day-old comparison groups, respectively, indicative of the alteration of the overall expression pattern of host lncRNAs caused by fungal invasion. This is similar to findings from other insects, such as Sarcophaga peregrina , Aedes aegypti , and B. mori in response to various pathogens or parasites [ 32 , 33 , 34 ]. In addition, 30 DElncRNAs (MSTRG.…”

Section: Discussionsupporting

confidence: 87%

Regulatory Roles of Long Non-Coding RNAs Relevant to Antioxidant Enzymes and Immune Responses of Apis cerana Larvae Following Ascosphaera apis Invasion

Guo,

Wang,

Guo

et al. 2023

IJMS

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Long non-coding RNAs (lncRNAs) play an essential part in controlling gene expression and a variety of biological processes such as immune defense and stress-response. However, whether and how lncRNAs regulate responses of Apis cerana larvae to Ascosphaera apis invasion has remained unclear until now. Here, the identification and structural analysis of lncRNAs in the guts of A. cerana worker larvae were conducted, and the expression profile of larval lncRNAs during the A. apis infection process was then analyzed, followed by an investigation of the regulatory roles of differentially expressed lncRNAs (DElncRNAs) in the host response. In total, 76 sense lncRNAs, 836 antisense lncRNAs, 184 intron lncRNAs, 362 bidirectional lncRNAs, and 2181 intron lncRNAs were discovered in the larval guts. Additionally, 30 known and 9 novel lncRNAs were potential precursors for 36 and 11 miRNAs, respectively. In the three comparison groups, 386, 351, and 272 DElncRNAs were respectively identified, indicating the change in the overall expression pattern of host lncRNAs following the A. apis invasion. Analysis of cis-acting effect showed that DElncRNAs in the 4-, 5-, and 6-day-old comparison groups putatively regulated 55, 30, and 20 up- and down-stream genes, respectively, which were involved in a series of crucial functional terms and pathways, such as MAPK signaling pathway, and cell process. Analysis showed that 31, 8, and 11 DElncRNAs as potential antisense lncRNAs may interact with 26, 8, and 9 sense-strand mRNAs. Moreover, investigation of the competing endogenous RNA (ceRNA) network indicated that 148, 283, and 257 DElncRNAs were putatively regulated. The expression of target genes by targeting corresponding DEmiRNAs included those associated with antioxidant enzymes and immune responses. These results suggested that DElncRNAs played a potential part in the larval guts responding to the A. apis infection through a cis-acting manner and ceRNA mechanisms. Our findings deepen our understanding of interactions between A. cerana larvae and A. apis and offer a basis for clarifying the DElncRNA-mediated mechanisms underlying the host response to fungal invasion.

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Section: Discussionsupporting

confidence: 87%

Regulatory Roles of Long Non-Coding RNAs Relevant to Antioxidant Enzymes and Immune Responses of Apis cerana Larvae Following Ascosphaera apis Invasion

Guo,

Wang,

Guo

et al. 2023

IJMS

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“…MiRNAs have been discovered to be involved in the resistance to viral invasion and replication in B. mori (Awais et al, 2021;Fan et al, 2023) and other species, such as S. frugiperda , Epinephelus coioides (Li et al, 2021) and Litopenaeus vannamei (Bao et al, 2020) and so on. In our previous study, we identified that miR-6498-5p was one of the differentially expressed miRNAs in B. mori upon BmNPV infection (Cao et al, 2024).…”

Section: Discussionmentioning

confidence: 99%

Bmo‐miR‐6498‐5p suppresses Bombyx mori nucleopolyhedrovirus infection by down‐regulating BmPLPP2 to modulate pyridoxal phosphate content in B. mori

Cao,

Kong,

Chen

et al. 2024

Insect Molecular Biology

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The RNA interference pathway mediated by microRNAs (miRNAs) is one of the methods to defend against viruses in insects. Recent studies showed that miRNAs participate in viral infection by binding to target genes to regulate their expression. Here, we found that the Bombyx mori miRNA, miR‐6498‐5p was down‐regulated, whereas its predicted target gene pyridoxal phosphate phosphatase PHOSPHO2 (BmPLPP2) was up‐regulated upon Bombyx mori nucleopolyhedrovirus (BmNPV) infection. Both in vivo and in vitro experiments showed that miR‐6498‐5p targets BmPLPP2 and suppresses its expression. Furthermore, we found miR‐6498‐5p inhibits BmNPV genomic DNA (gDNA) replication, whereas BmPLPP2 promotes BmNPV gDNA replication. As a pyridoxal phosphate (PLP) phosphatase (PLPP), the overexpression of BmPLPP2 results in a reduction of PLP content, whereas the knockdown of BmPLPP2 leads to an increase in PLP content. In addition, exogenous PLP suppresses the replication of BmNPV gDNA; in contrast, the PLP inhibitor 4‐deoxypyridoxine facilitates BmNPV gDNA replication. Taken together, we concluded that miR‐6498‐5p has a potential anti‐BmNPV role by down‐regulating BmPLPP2 to modulate PLP content, but BmNPV induces miR‐6498‐5p down‐regulation to promote its proliferation. Our findings provide valuable insights into the role of host miRNA in B. mori–BmNPV interaction. Furthermore, the identification of the antiviral molecule PLP offers a novel perspective on strategies for preventing and managing viral infection in sericulture.

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“…Several lines of evidence have demonstrated that circRNAs exert regulatory functions in diverse aspects of vertebrates such as growth, development, metabolism, and immunity through cis-acting effect [50][51][52][53]. For instance, Weigelt et al [51] previously detected the continuous up-regulation of the circRNA transcribed from the sulfateless gene (circSfl) in the brain and muscle of insulin mutant flies, and the circSfl overexpression prolonged the host lifespan.…”

Section: Discussionmentioning

confidence: 99%

Novel Insights into the circRNA-Modulated Developmental Mechanism of Western Honey Bee Larval Guts

Zhang,

Fan,

Zang

et al. 2023

Insects

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Circular RNAs (circRNAs) are a class of novel non-coding RNAs (ncRNAs) that play essential roles in the development and growth of vertebrates through multiple manners. However, the mechanism by which circRNAs modulate the honey bee gut development is currently poorly understood. Utilizing the transcriptome data we obtained earlier, the highly expressed circRNAs in the Apis mellifera worker 4-, 5-, and 6-day-old larval guts were analyzed, which was followed by an in-depth investigation of the expression pattern of circRNAs during the process of larval guts development and the potential regulatory roles of differentially expressed circRNAs (DEcircRNAs). In total, 1728 expressed circRNAs were detected in the A. mellifera larval guts. Among the most highly expressed 10 circRNAs, seven (novel_circ_000069, novel_circ_000027, novel_circ_000438, etc.) were shared by the 4-, 5-, and 6-day-old larval guts. In addition, 21 (46) up-regulated and 22 (27) down-regulated circRNAs were, respectively, screened in the Am4 vs. Am5 (Am5 vs. Am6) comparison groups. Additionally, nine DEcircRNAs, such as novel_circ_000340, novel_circ_000758 and novel_circ_001116, were shared by these two comparison groups. These DEcircRNAs were predicted to be transcribed from 14 and 29 parental genes; these were respectively annotated to 15 and 22 GO terms such as biological regulation and catalytic activity as well as 16 and 21 KEGG pathways such as dorsoventral axis formation and apoptosis. Moreover, a complicated competing endogenous RNA (ceRNA) network was observed; novel_circ_000838 in the Am4 vs. Am5 comparison group potentially targeted ame-miR-6000a-3p, further targeting 518 mRNAs engaged in several developmental signaling pathways (e.g., TGF-beta, hedgehog, and wnt signaling pathway) and immune pathways (e.g., phagosome, lysosome, and MAPK signaling pathway). The results demonstrated that the novel_circ_000838-ame-miR-6000a-3p axis may plays a critical regulatory part in the larval gut development and immunity. Furthermore, back-splicing sites of six randomly selected DEcircRNAs were amplified and verified by PCR; an RT-qPCR assay of these six DEcircRNAs confirmed the reliability of the used high-throughput sequencing data. Our findings provide a novel insight into the honey bee gut development and pave a way for illustration of the circRNA-modulated developmental mechanisms underlying the A. mellifera worker larval guts.

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Multi-omics study and ncRNA regulation of anti-BmNPV in silkworms, Bombyx mori: an update

Cited by 5 publications

References 110 publications

Regulatory Roles of Long Non-Coding RNAs Relevant to Antioxidant Enzymes and Immune Responses of Apis cerana Larvae Following Ascosphaera apis Invasion

Regulatory Roles of Long Non-Coding RNAs Relevant to Antioxidant Enzymes and Immune Responses of Apis cerana Larvae Following Ascosphaera apis Invasion

Bmo‐miR‐6498‐5p suppresses Bombyx mori nucleopolyhedrovirus infection by down‐regulating BmPLPP2 to modulate pyridoxal phosphate content in B. mori

Novel Insights into the circRNA-Modulated Developmental Mechanism of Western Honey Bee Larval Guts

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