Multi-parametric study of temperature and thermal damage of tumor exposed to high-frequency nanosecond-pulsed electric fields based on finite element simulation

Mi, Yan; Rui, Shaoqin; Li, Chengxiang; Yao, Chenguo; Xu, Jun; Bian, Changhao; Tang, Xuefeng

doi:10.1007/s11517-016-1589-3

Cited by 28 publications

(14 citation statements)

References 54 publications

(74 reference statements)

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“…However, it is a delicate balance between an effective ablation and thermal effects or damage. The occurrence of thermal injury during a regular IRE treatment and the impact of a metal stent on the local temperature distribution and thermal injury remains controversial 4,13,[16][17][18][19][20][21]23,26,[32][33][34] . Severe complications during IRE of a pancreas tumour were presumed to be related to heating of the metal stent in a case report 13 .…”

Section: Discussionmentioning

confidence: 99%

Effect of irreversible electroporation parameters and the presence of a metal stent on the electric field line pattern

Hogenes

Slump

Scholten

et al. 2020

Sci Rep

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The final ablation zone created with irreversible electroporation (IRE) depends on the size, shape and strength of the electric field that is influenced by several parameters. A profound understanding of the effect of IRE parameter alterations on the electric field are a prerequisite for a safe and effective treatment. Here, we demonstrate a semolina in castor oil model that enables visualization of the static electric field developed by a high-voltage generator between two needle-electrodes. We intuitively visualize the variation in electric field line pattern for selected IRE parameters; active needle length, inter-needle distance, applied voltage and presence of a nearby metal stent, by cameras in three dimensions. The observations were compared to and supported by two-dimensional numerical simulations of the electric field. Our semolina model visualizes the disturbance of the electric field by a metal stent, potentially leading to an incomplete tumour ablation between the needles. The reduction in electric field strength and the area at risk for incomplete tumour ablation are confirmed by the numerical simulations. The semolina model provides insight in the fundamental physics of the electric field, the effect of alterations in IRE parameter combinations and presence of a metal stent within the ablation zone. Irreversible electroporation (IRE) is theoretically a non-thermal ablation technique, which makes it especially suitable for ablation of tumours near vital structures, such as locally advanced pancreatic tumours 1-3. IRE uses a high-voltage external electric field and electric pulses of microsecond duration to change the transmembrane potential of tumour cells, resulting in permanent permeabilization of the cell membrane 4-6. Membrane permeabilization disturbs the cell's mechanisms to maintain homeostasis, finally leading to cell death via a necrotic pathway presumably or apoptosis 4,7-9. Tumour cells are ablated while the extracellular matrix, collagen and elastic fibres, such as blood vessels and bile ducts remain intact 9,10. Biliary drainage is frequently required in patients with pancreatic tumours. The use of a metal stent is usually preferred because of fewer stent-related complications (e.g. cholangitis) and less stent dislocations compared to plastic stents 11,12. It is currently unknown whether IRE can be safely and effectively applied in patients with a metal stent in situ close to the ablation site. Detrimental effects of a metal object on IRE outcomes have been suggested in literature. In a case report, severe complications have been described after IRE treatment in the proximity of a metal stent, potentially caused by unintended thermal effects 13. A retrospective clinical study demonstrated that IRE near small metal surgical clips (< 1 cm to tumour margin or needle electrodes) resulted in distortion of the electric field and inadequate coverage of the tumour, a less effective cell death and reduced treatment efficacy 14. The presence of a metal clip was also significantly related to local ...

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Section: Discussionmentioning

confidence: 99%

Effect of irreversible electroporation parameters and the presence of a metal stent on the electric field line pattern

Hogenes

Slump

Scholten

et al. 2020

Sci Rep

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“…Gianulis et al 27 tested cytotoxic efficiency of nsPEF for different cancers and found no apparent correlation with cell types. Recent studies 28 , 29 showed that, beside nsPEF dosage, temperature is another important issue when applying nsPEF in practice. Pliquett et al 2 measured the real time Joule heating and proved that nsPEF ablation under a certain parameter (i.e.…”

Section: Discussionmentioning

confidence: 99%

“…pulse duration of 300 ns and electric field strength of 40 kV/cm) produced no obvious heat production or temperature increase. Mi et al 28 expanded the measurement to a multi-parametric setting (i.e. nsPEF parameter ranging from 1 to 4 kV, pulse width ranging from 50 to 500 ns, and repetition frequency between 100 kHz and 1 MHz).…”

Section: Discussionmentioning

confidence: 99%

Activation of Anti-tumor Immune Response by Ablation of HCC with Nanosecond Pulsed Electric Field

Xu¹,

Zhang²,

Miao³

et al. 2017

Journal of Clinical and Translational Hepatology

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Locoregional therapy is playing an increasingly important role in the non-surgical management of hepatocellular carcinoma (HCC). The novel technique of non-thermal electric ablation by nanosecond pulsed electric field has been recognized as a potential locoregional methodology for indicated HCC. This manuscript explores the most recent studies to indicate its unique anti-tumor immune response. The possible immune mechanism, termed as nano-pulse stimulation, was also analyzed.

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“…Theoretical simulations suggested that multiple pulses may cause Joule heating [ 17 ]. In order to determine whether heat played a part in the cellular response, the temperature rise in the cell suspension was measured using a thermocouple immediately after nsEP exposure (Guangzhou Sungun Meas.…”

Section: Methodsmentioning

confidence: 99%

Nanosecond Electric Pulses Induce Early and Late Phases of DNA Damage and Cell Death in Cisplatin-Resistant Human Ovarian Cancer Cells

Qian

2018

BioMed Research International

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Chemoresistance is a challenge for management of ovarian cancer, and therefore the response of resistant cells to nanosecond electric pulses (nsEP) was explored. Human ovarian cancer cell line COC1 and the cisplatin-resistant subline COC1/DDP were subjected to nsEP (32 ns, 10 kV/cm, 10 Hz pulse repletion frequency, and 10 min exposure duration), and then the cellular responses were followed. The percentages of dead cells and of comet-formed cells in the alkaline assay displayed two peak levels (i.e., 2 and 8 h after nsEP exposure), with the highest value noted at 8 h; the percentage of comet-formed cells in the neutral assay was increased at 8 h; the apoptotic percentage was increased at 8 h, with collapse of the mitochondrial membrane potential and the activation of caspase-3 and caspase-9. The comet assay demonstrated DNA single-strand break at 2 h and double-strand break at 8 h. nsEP resulted in lower cytotoxicity in COC1/DDP cells compared with COC1 cells. These findings indicated that nsEP induced early and late phases of DNA damage and cell death, and these two types of cell death may have distinct applications to treatments of chemoresistant ovarian cancers.

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Multi-parametric study of temperature and thermal damage of tumor exposed to high-frequency nanosecond-pulsed electric fields based on finite element simulation

Cited by 28 publications

References 54 publications

Effect of irreversible electroporation parameters and the presence of a metal stent on the electric field line pattern

Effect of irreversible electroporation parameters and the presence of a metal stent on the electric field line pattern

Activation of Anti-tumor Immune Response by Ablation of HCC with Nanosecond Pulsed Electric Field

Nanosecond Electric Pulses Induce Early and Late Phases of DNA Damage and Cell Death in Cisplatin-Resistant Human Ovarian Cancer Cells

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