Multi-pin contact drawing enables production of anisotropic collagen fiber substrates for alignment of fibroblasts and monocytes

Verma, Surendra Kumar; Yaghoobi, Hessameddin; Slaine, Patrick D; Baldwin, Samuel J.; Rainey, Jan K.; Kreplak, Laurent; Frampton, John P.

doi:10.1016/j.colsurfb.2022.112525

Cited by 8 publications

(21 citation statements)

References 71 publications

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“…Contact-drawn fibers dry within 1–2 s of forming, allowing the fibers to be rapidly assembled into nonwoven textiles through consecutive fiber elongation cycles. , The number of fibers produced in each elongation cycle scales with the number of pins present on the pin array tool, and the angular orientation of fibers between layers can be controlled by rotating the substrate on which the fibers are collected. In the case of PEO-Col fibers, the collagen self-assembles into a stable protein fiber upon dissolution of the PEO . However, as PVA is water soluble, it is necessary to determine an optimal crosslinking strategy to stabilize contact-drawn PVA fibers in aqueous environments.…”

Section: Resultsmentioning

confidence: 99%

“…In addition, based on previous reports of quercetin contributing to differentiation and neurite formation in PC12 cells, , we hypothesized that sustained quercetin release would lead to improved PC12 cell differentiation when combined with fibers that support the anisotropic growth of adherent cells. , Thus, PC12 cells were cultured on substrates containing PEO-Col fibers only, PEO-Col/PVA fibers, and PEO-Col/quercetin-loaded PVA fibers. 30 min after cell seeding on PEO-Col fibers, PC12 cells exhibited rounded morphologies but appeared to interact with the PEO-Col fibers as loose aggregates (Figure A).…”

Section: Resultsmentioning

confidence: 99%

“…To prepare the PEO-Col fibers, PEO powder (8 MDa; Sigma-Aldrich) was dissolved in type I collagen solution (6 mg/mL in 0.01 M HCl, Collagen Solutions). The PEO-Col solution was prepared as described previously . PVA fibers were prepared as described above.…”

Section: Methodsmentioning

confidence: 99%

“…36 This approach has been used previously with dextran as the high molecular weight polymer to load antibiotics, hemostatic agents, and collagen into fibers, 37,38 and with polyethylene oxide to load collagen and silver nanoparticles into fibers. 39,40 Here, we applied the contact drawing approach to generate PVA fibers for the release of quercetin, a plant flavonoid with anti-inflammatory and neuroprotective effects. 41,42 Quercetin is best known for its antioxidative 43,44 and antimicrobial properties.…”

mentioning

confidence: 99%

“…The PEO-Col solution was prepared as described previously. 39 PVA fibers were prepared as described above. The elastomeric support was also prepared as described previously.…”

mentioning

confidence: 99%

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Loading and Release of Quercetin from Contact-Drawn Polyvinyl Alcohol Fiber Scaffolds

Visser

Verma

Rainey

et al. 2022

ACS Pharmacol. Transl. Sci.

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Polymeric drug releasing systems have numerous applications for the treatment of chronic diseases and traumatic injuries. In this study, a simple, cost-effective, and scalable method for dry spinning of crosslinked polyvinyl alcohol (PVA) fibers is presented. This method utilizes an entangled solution of PVA to form liquid bridges that are drawn into rapidly drying fibers through extensional flow. The fibers are crosslinked by a one-pot reaction in which glyoxal is introduced to the PVA solution prior to contact drawing. Failure analysis of fiber formation is used to understand the interplay of polymer concentration, glyoxal concentration, and crosslinking time to identify appropriate formulations for the production of glyoxal-crosslinked PVA fibers. The small molecule quercetin (an anti-inflammatory plant flavonoid) can be added to the one-pot reaction and is shown to be incorporated into the fibers in a concentration-dependent manner. Upon rehydration in an aqueous medium, the glyoxal-crosslinked PVA fiber scaffolds retain their morphology and slowly degrade, as measured over the course of 10 days. As the scaffolds degrade, they release the loaded quercetin, reaching a cumulative release of 56 ± 6% of the loaded drug after 10 days. The bioactivity of the released quercetin is verified by combining quercetin-loaded fibers with contact-drawn polyethylene oxide-type I collagen (PEO-Col) fibers and monitoring the growth of PC12 cells on the fibers. PC12 cells readily attach to the PEO-Col fibers and display increased nerve growth factor-induced elongation and neurite formation in the presence of quercetin-loaded PVA fibers relative to substrates formed from only PEO-Col fibers or PEO-Col and PVA fibers without quercetin.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

mentioning

confidence: 99%

“…The PEO-Col solution was prepared as described previously. 39 PVA fibers were prepared as described above. The elastomeric support was also prepared as described previously.…”

mentioning

confidence: 99%

See 3 more Smart Citations

Loading and Release of Quercetin from Contact-Drawn Polyvinyl Alcohol Fiber Scaffolds

Visser

Verma

Rainey

et al. 2022

ACS Pharmacol. Transl. Sci.

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Nonwoven Hemostatic Dressings Formed by Contact Drawing of Interposed Polyethylene Oxide (PEO)‐Fibrinogen and PEO‐Thrombin Microfibers

Verma

Yaghoobi

Kreplak

et al. 2022

Adv Materials Inter

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Textiles containing interposed layers of polyethylene oxide (PEO)‐fibrinogen and PEO‐thrombin fibers are explored as biomaterials for hemostasis. The PEO‐fibrinogen and PEO‐thrombin fibers are formed by contact drawing, an approach that uses an entangled polymer solution and a pin array to form fibers by extension of liquid bridges. The interposition of the PEO‐fibrinogen and PEO‐thrombin fibers results in polymerization of a fibrin hydrogel mesh once the textile is hydrated. This fibrin hydrogel mesh displays the expected bands and diffraction peaks by Fourier‐transform infrared spectromicroscopy and X‐ray diffraction, respectively. The functionality of the hemostatic textiles formed from the interposed PEO‐fibrinogen and PEO‐thrombin fibers is demonstrated by analyzing human blood hemolysis, complement activation, protein adsorption, and platelet and leukocyte adhesion, indicating compatibility with human blood (hemolysis ratio <5%), with minimal inflammatory response (levels of terminal complement complex equivalent to plasma). The cytocompatibility and potential for cell remodeling of the fibrin hydrogel mesh formed by this process is evaluated with human dermal fibroblasts and human keratinocytes and it is found that both cell types attach and grow on the fibrin mesh. Finally, a whole blood clotting time of less than 30 s suggests a potential use of this material in hemorrhage control.

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A Comparative Study of the Effects of Different Crosslinking Methods on the Physicochemical Properties of Collagen Multifilament Bundles

Rasouli,

Yaghoobi,

Frampton

2024

ChemPhysChem

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Crosslinking is usually required to improve the mechanical properties and stability of collagen‐based scaffolds. Introducing exogenous crosslinks into collagen may however affect the collagen structure. Since the architecture of collagen is tied to its functionality, it is important to study the effect of crosslinking and to select a crosslinking method that preserves the collagen structure and mechanical properties. This study compares different physical (ultraviolet light, UVC) and chemical (genipin, carbodiimide (EDC), and glutaraldehyde) crosslinking methods on contact‐drawn collagen multifilament bundles designed to recapitulate the structure of a tendon. The presence of collagen and the formation of well‐ordered collagen structures are confirmed by attenuated total reflectance Fourier‐transform infrared spectromicroscopy and wide‐angle X‐ray scattering for all crosslinking methods. The morphology of the collagen multifilament bundles is similar across crosslinking methods. Swelling of the multifilament bundles is dramatically reduced following crosslinking and varies by crosslinking method, with genipin‐ and carbodiimide‐crosslinked specimens swelling the least. Ultimate tensile strength (UTS) and Young’s modulus significantly improve for all crosslinked specimens compared to non‐crosslinked specimens. Glutaraldehyde crosslinked collagen multifilament bundles display the highest UTS values ranging from 33.82±0.0 MPa to 45.528±0.757 MPa.

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Multi-pin contact drawing enables production of anisotropic collagen fiber substrates for alignment of fibroblasts and monocytes

Cited by 8 publications

References 71 publications

Loading and Release of Quercetin from Contact-Drawn Polyvinyl Alcohol Fiber Scaffolds

Loading and Release of Quercetin from Contact-Drawn Polyvinyl Alcohol Fiber Scaffolds

Nonwoven Hemostatic Dressings Formed by Contact Drawing of Interposed Polyethylene Oxide (PEO)‐Fibrinogen and PEO‐Thrombin Microfibers

A Comparative Study of the Effects of Different Crosslinking Methods on the Physicochemical Properties of Collagen Multifilament Bundles

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