Multi-purpose optical biosensors for real-time detection of bacteria, viruses and toxins

Bhatta, D.; Stadden, E.; Hashem, Elham Y.; Sparrow, I.J.G.; Emmerson, G.D.

doi:10.1016/j.snb.2010.05.040

Cited by 46 publications

(25 citation statements)

References 21 publications

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“…[4]. Antibody-based biosensors have become more useful, and most of the biosensors developed are designed based on electrochemical, optical or micro-gravimetric detection [15][16][17][18]. In recent decades, biosensors/biochips have been envisaged to compensate and complement conventional diagnostic methods due to their easy operation and transport; they require no reagent and provide results in a few minutes [5][6][7].…”

Section: Introductionmentioning

confidence: 99%

A novel biosensor based on serum antibody immobilization for rapid detection of viral antigens

Huy

Hạnh

Thuy

et al. 2011

Talanta

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In this paper, we represent a label-free biosensor based on immobilization of serum antibodies for rapid detection of viral antigens. Human serum containing specific antibodies against Japanese encephalitis virus (JEV) was immobilized on a silanized surface of an interdigitated sensor via protein A/glutaraldehyde for electrical detection of JEV antigens. The effective immobilization of serum antibodies on the sensor surface was verified by Fourier transform infrared spectrometry and fluorescence microscopy. The signal of the biosensor obtained by the differential voltage converted from the change into non-Faradic impedance resulting from the specific binding of JEV antigens on the surface of the sensor. The detection analyzed indicates that the detection range of this biosensor is 1-10 μg/ml JEV antigens, with a detection limit of 0.75 μg/ml and that stable signals are measured in about 20 min. This study presents a useful biosensor with a high selectivity for rapid and simple detection of JEV antigens, and it also proposes the biosensor as a future diagnostic tool for rapid and direct detection of viral antigens in clinical samples for preliminary pathogenic screenings in the case of possible outbreaks.

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Section: Introductionmentioning

confidence: 99%

A novel biosensor based on serum antibody immobilization for rapid detection of viral antigens

Huy

Hạnh

Thuy

et al. 2011

Talanta

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“…The high finesse optical elements fabricated here by FLICE demonstrated the novel utility of assembling open‐structured FPIs into compact stacked ORAs without relying on tedious nano‐precision assembly methods. ORA sensing may therefore provide new compact elements for detection of minute changes in liquid density , viscosity , toxic organic concentrations with extension to functionalized detection by binding selective antibodies to the sensing surfaces , and other parameters connected to the refractive index. ORAs may find importance in optical communication networks (band filters, polarizers, and optical switches), chemical and biological sensors, biomedical probes, and low‐cost health care products.…”

Section: Significancementioning

confidence: 99%

Chemical‐assisted femtosecond laser writing of optical resonator arrays

Haque

Herman

2015

Laser & Photonics Reviews

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The design of micro‐optical resonator arrays are introduced and tailored towards refractive index sensing applications, building on the previously unexplored benefits of open dielectric stacks. The resonant coupling of identical hollow cavities present strong and narrow spectral resonance bands beyond that available with a single Fabry Perot interferometer. Femtosecond laser irradiation with selective chemical etching is applied to precisely fabricate stacked and waveguide‐coupled open resonators into fused silica, taking advantage of small 12 nm rms surface roughness made available by the self‐alignment of nanograting planes. Refractive index sensing of methanol‐water solutions confirm a very attractive sensing resolution of 6.5 × 10−5 RIU. Such high finesse optical elements open a new realm of optofluidic sensing and integrated optical circuit concepts for detecting minute changes in sample properties against a control solution that may find importance in chemical and biological sensors, telecom sensing networks, biomedical probes, and low‐cost health care products.

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“…Biological materials (nucleotides, proteins, lipid bilayers, and vesicles) and chemicals can be immobilized via surface anchoring, 129,130 and the functionalized chip surfaces can be used as nanoscale sensors for detection of molecules (e.g., bacteria, toxin, virus, protein, antibody, DNA, ligand, and lipid). [149][150][151] For example, PDMS-based microfluidic devices were functionalized via a series of SAMs comprising 3-mercaptopropyltrimethoxysilane (MPTMS), N-y-maleimidobutyryloxy succinimide ester (GMBS), neutravidin, and biotinylated aEpCAM. 152 This novel surface functionalization has a great potential for the simple, sensitive and cost-effective detection of EpCAM-expressing cells such as CTCs.…”

Section: Self-assembly-based Diagnosticsmentioning

confidence: 99%

Bioinspired Engineering of Multifunctional Devices

Pearson¹,

Myung²,

Hong³

2014

Handbook of Biomimetics and Bioinspiration

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Biomimicry, which takes advantage of naturally occurring phenomena, has recently become one of the most promising strategies to create unprecedentedly effective biomedical devices. However, the inherent complexity of biomolecules presents a tremendous challenge to scientists and engineers to successfully mimic their structures and related functions. With an aim to aid in the design and fabrication of effective biomimetic materials and devices, this chapter describes the basics of various physiological phenomena including multivalent binding, molecular self-assembly, stimuli-responsiveness, transfer of genetic materials, tissue regeneration/wound healing, and natural surface architecture and adhesiveness. In addition, a number of natural and man-made materials that derive inspiration from such natural processes and their applications are also discussed to provide a guideline to help successfully engineer bioinspired, multifunctional biomedical devices.

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Multi-purpose optical biosensors for real-time detection of bacteria, viruses and toxins

Cited by 46 publications

References 21 publications

A novel biosensor based on serum antibody immobilization for rapid detection of viral antigens

A novel biosensor based on serum antibody immobilization for rapid detection of viral antigens

Chemical‐assisted femtosecond laser writing of optical resonator arrays

Bioinspired Engineering of Multifunctional Devices

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