Multi-residue method for the determination of thyreostats in urine samples using liquid chromatography coupled to tandem mass spectrometry after derivatisation with 3-iodobenzylbromide

Pinel, Gaud; Bichon, Emmanuelle; Pouponneau, Karine; Maume, Daniel; André, François; Bizec, Bruno Le

doi:10.1016/j.chroma.2005.06.055

Cited by 50 publications

(82 citation statements)

References 12 publications

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“…The positive ionisation was used in the method, as only this ionisation mode is suitable for all investigated compounds. Negative ionisation, used mainly for thiouracil and its derivatives, provides unsatisfactory results for tapazole (23). In this method, for each analyte a precursor ion (parent mass) and two fragments (corresponding to strong and weak ion) are monitored ( Table 1).…”

Section: Discussionmentioning

confidence: 99%

“…The low molecular weight, high polarity, and the existence of tautomeric forms of thyreostats caused difficulties associated with the analytical determination of this group of compounds for many years. These inconveniences are reduced by application of derivatisation, and 3-iodo-benzylbromide has been tested and selected as the most efficient derivatisation agent when detection is performed by LC-MS/MS (23). When analysis is performed by GC method, derivatisation converts the analytes into volatile compounds, suitable for this technique (31).…”

Section: Introductionmentioning

confidence: 99%

“…A method for the identification of eight thyreostatic drugs in animal feeding stuff samples was published in 2005 (23). Thyreostats were extracted with methanol, and derivatisation with 3-iodobenzylbromide in a strong basic condition was conducted.…”

Section: Introductionmentioning

confidence: 99%

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Determination of the Thyreostats in Animal Feeding Stuffs Using Liquid Chromatography-Tandem Mass Spectrometry

Woźniak

Witek

Matraszek-Żuchowska

et al. 2014

Bulletin of the Veterinary Institute in Pulawy

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A rapid liquid chromatography tandem mass spectrometry method was developed and validated to detect and confirm five thyreostatic drugs: tapazole, thiouracil, methylthiouracil, propylthiouracil, and phenylthiouracil in animal feeding stuff samples. Thyreostats were extracted from feed with methanol, and then degreasing of the extract with petroleum ether was performed, followed by the derivatisation of the compounds with 3-iodobenzylbromide in basic medium (pH 8.0). The derivatives were extracted with diethyl ether and analysed by gradient elution on a Poroshell 120-EC C18 column with triple quadrupole MS detection with turbo spray source in positive ionisation mode. The method was validated in accordance with the Commission Decision 2002/657/EC. For validation level of 10 μg kg -1 , the recovery ranged from 82% to 97.5% for all examined compounds. The repeatability and reproducibility did not exceed the limit of 20% for all analytes. The linearity was good for all thyreostats in the whole range of tested concentrations, as proved by the correlation coefficients greater than 0.99. The decision limits (CC) ranged from 1.63 μg kg -1 to 3.95 μg kg -1 , whereas the detection capabilities (CC) ranged from 2.74 μg kg -1 to 6.73 μg kg -1 . The developed analysis is sensitive and robust, and therefore useful for quantification and confirmation of thyreostats in residue control programme.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Determination of the Thyreostats in Animal Feeding Stuffs Using Liquid Chromatography-Tandem Mass Spectrometry

Woźniak

Witek

Matraszek-Żuchowska

et al. 2014

Bulletin of the Veterinary Institute in Pulawy

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“…Pinel et al were able to isolate seven TS residues from various biological matrices, including urine, muscle, liver, thyroid, animal feed, faeces and hair [186]. Solid samples were freeze-dried, extracted with MeOH and evaporated.…”

Section: Thyreostatsmentioning

confidence: 99%

Current trends in sample preparation for growth promoter and veterinary drug residue analysis

Kinsella

O’Mahony

Malone

et al. 2009

Journal of Chromatography A

181

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A comprehensive review is presented on the current trends in sample preparation for isolation of veterinary drugs and growth promotors from foods. The objective of the review is to firstly give an overview of the sample preparation techniques that are applied in field. The review will focus on new techniques and technologies, which improve efficiency and coverage of residues. The underlying theme to the paper is the developments that have been made in multi-residue methods and particularly multi-class methods for residues of licensed animal health products, which have been developed in the last couple of years. The role of multi-class methods is discussed and how they can be accommodated in future residue surveillance.

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“…The examinations were performed using mainly a high performance thin layer chromatography, with detection limits at 50 µg L -1 (6). A recent great progress in the efficiency of analytical methods for thyreostatic drugs determination has lead to a significant reduction of detection limits (12,13,15). As a result, in some European countries, including Poland, one of thyreostatics -thiouracil (TU) is detected in urine samples of slaughtered animals (7).…”

mentioning

confidence: 99%

Natural Occurrence of Thiouracil in Urine of Livestock in Poland

Woźniak

Witek

Żmudzki

et al. 2012

Bulletin of the Veterinary Institute in Pulawy

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Natural occurrence of thiouracil in bovine and swine urine in Poland was investigated. Under the national residue control programme, 537 urine samples were tested. In 77 samples (14.3%) thiouracil was detected above decision limit CCα (0.91 µg L -1 ), including eight samples over the recommended concentration of 10 µg L -1 . Of the bovine urine samples, 95 and 99 percentiles have thiouracil concentration below 4.50 and 14.85 μg L -1 ,and of porcine samples below 2.35 and 6.80 µg L -1 , respectively.

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Multi-residue method for the determination of thyreostats in urine samples using liquid chromatography coupled to tandem mass spectrometry after derivatisation with 3-iodobenzylbromide

Cited by 50 publications

References 12 publications

Determination of the Thyreostats in Animal Feeding Stuffs Using Liquid Chromatography-Tandem Mass Spectrometry

Determination of the Thyreostats in Animal Feeding Stuffs Using Liquid Chromatography-Tandem Mass Spectrometry

Current trends in sample preparation for growth promoter and veterinary drug residue analysis

Natural Occurrence of Thiouracil in Urine of Livestock in Poland

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