Multi-Residue Method for the Screening of Benzimidazole and Metabolite Residues in the Muscle and Liver of Sheep and Cattle Using HPLC/PDAD with DVB-NVP-SO3Na for Sample Treatment

Xiong, Lin; Huang, Lele; Shimo, Shimo Peter; Li, Weihong; Yang, Xiaolin; Yan, Ping

doi:10.1007/s10337-016-3144-7

Cited by 3 publications

(3 citation statements)

References 14 publications

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“…In this work, divinylbenzene-N-vinyl-2-pyrrolidinone polymer Poly(DVB-co-NVP) microspheres were prepared by suspension polymerization. [36] The structure, morphology and element composition of Poly(DVB-co-NVP) microspheres were analyzed by advanced testing tools such as fourier transform infrared spectrometer (FT-IR), X-ray photoelectron spectrometer (XPS), scanning electron microscope (SEM) and N 2 adsorption/desorption. The Poly(DVB-co-NVP) microspheres were made into solid-phase extraction cartridges to extract 4 steroid hormones in different water samples.…”

Section: Introductionmentioning

confidence: 99%

Preparation of Amphiphilic Solid Phase Extraction Material and Its Application in Aqueous Steroid Hormones Extraction

et al. 2023

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The steroid hormones have attracted increasing attentions in aquatic environment because of their endocrine disrupting effects at trace concentrations. In this work, amphiphilic solid phase extraction material of divinylbenzene‐N‐vinyl‐2‐pyrrolidinone polymer microsphere was synthesized by free radical polymerization. The adsorption of 4 steroid hormones in aqueous solution by prepared amphiphilic microsphere was determined by high‐performance liquid chromatography‐tandem mass spectrometry method. When methyl cellulose and sodium dodecyl sulfonate were used as dispersants, 2,2’‐azobis(2‐methylpropionitrile) was used as initiator, the amphiphilic microsphere was successfully prepared at 80 °C for 6 h. The amphiphilic microsphere has high specific surface area of 774 m2 g−1 with uniform particle size, which is comparable to the commercial solid phase extraction fillers. The amphiphilic microsphere has been successfully applied to the enrichment and detection of 4 steroid hormones (17α‐hydroxyprogesterone, metandienone, norethindrone and boldenone) in natural water. The recoveries of 4 steroid hormones ranged from 83.7 % to 114 %, and the limit of detection were in the range of 0.70–13.5 ng L−1.

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Section: Introductionmentioning

confidence: 99%

Preparation of Amphiphilic Solid Phase Extraction Material and Its Application in Aqueous Steroid Hormones Extraction

et al. 2023

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“…Conventional drugs will have side effects or toxic reactions when they are used in the treatment of certain diseases and the substance produced by the organism is the antibody itself, which does not need to worry too much about the problem of toxicity, so it is widely used in medicine. However, the traditional single antibody has the problems of large volume, complex preparation process and poor stability [11] , which makes it inconvenient to use. Nano antibody belongs to one kind of antibody, which can bind antigen well.…”

mentioning

confidence: 99%

“…In order to save the life of patients with muscle atrophy and improve their quality of life, bivalent sheep nano antibody is used in this paper, which affects the shape and weight of limb muscles, so as to gradually restore the muscle shape of patients with muscle atrophy and improve their muscle movement ability. Moreover, through the experiment, bivalent sheep nano antibody has high safety and low side effects after long-term use [11] , so it can be used in medicine to treat muscular atrophy symptoms and improve the movement ability of muscles.…”

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confidence: 99%

Effect of Sheep Nano Antibody on Muscle Movement of Human Tissue Flap

Yu¹

2021

ijps

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In this study, the effect of sheep nano antibody on the movement of human tissue flap muscle is studied. Firstly, the non-toxic effect of bivalent sheep nano antibody on mouse muscle cells is investigated and then the effect of the antibody on the movement of mouse tissue flap muscle is analyzed. In the repeated experiment of injecting bivalent sheep myostatin nano antibody into mice, 30 healthy Kunming mice of 1 mo old and weighing 15 g without specific pathogen are selected. When using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide method to detect the myoblast toxicity of mouse immortalized mouse myoblast cell line C2C12, phosphate buffered saline control group, blank control group and the observation group with concentration of 10, 20, 30 and 60 μg/ml of bivalent nano antibody membrane proteome array are set respectively. In the repeated experiment of bivalent sheep myostatin nano antibody on mice, it is divided into phosphate buffered saline control group, monovalent group and bivalent group. Each animal in phosphate buffered saline group is injected with 30 μl phosphate buffered saline, each animal in monovalent group is injected with 30 μl (60 μg) monovalent sheep myostatin nano antibody and each animal in bivalent group is injected with 30 μl (60 μg) bivalent sheep myostatin nano antibody. The weight of the single hind limb of the bivalent sheep myostatin nano antibody group is higher than that of the monovalent sheep myostatin nano antibody group and the control group, and the muscle morphology of the hind limb of the bivalent sheep myostatin nano antibody group is significantly higher than that of the monovalent sheep myostatin nano antibody group and the control group, which is more plump. The results show that sheep nano antibody is non-toxic to human tissue flap muscle, which can promote the growth of human tissue flap muscle and enhance muscle movement ability.

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Development of immunoassays for multi-residue detection of small molecule compounds

Cui

Jin

et al. 2018

Food and Agricultural Immunology

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Multi-Residue Method for the Screening of Benzimidazole and Metabolite Residues in the Muscle and Liver of Sheep and Cattle Using HPLC/PDAD with DVB-NVP-SO3Na for Sample Treatment

Cited by 3 publications

References 14 publications

Preparation of Amphiphilic Solid Phase Extraction Material and Its Application in Aqueous Steroid Hormones Extraction

Preparation of Amphiphilic Solid Phase Extraction Material and Its Application in Aqueous Steroid Hormones Extraction

Effect of Sheep Nano Antibody on Muscle Movement of Human Tissue Flap

Development of immunoassays for multi-residue detection of small molecule compounds

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