Multi-Resistant Carbapenem-Insensitive Gramnegative Bacteria in Patients With Solid Tumors

Abstract

Infections caused by multiresistant gram-negative bacteria are one of the major problems in the treatment of cancer patients. Strains with mechanisms of resistance mediated by carbapenemases represent a particular threat since they spread rapidly and are characterized by high frequency of occurrence of multiresistance to antimicrobial agents. Here we show that 14 out of 399 gram-negative bacteria (3,5 %), isolated from clinical specimens of 11 patients with solid tumors (n=581) in a hospital of federal level in January 2015-April 2016 were carbapenem-insusceptible. Among them 3 isolates of Klebsiella pneumonia, 2 Enterobacter cloacae, 2 Pseudomonas aeruginosa and 7 Acinetobacter baumannii. All 14 strains were resistant to a wide range of antimicrobial agents including beta-lactams, aminoglycosides, monobactams and fluoroquinolones. The only antimicrobial agent to which all but one Exloacae strain remained susceptible was colistin. This strain was insusceptible to all 10 antimicrobial agents tested in the study, including tigecycline. We observed two cases of infection of a single patient by 2-3 distinct species of multidrug-resistant gram-negative bacteria. In 79 % of the strains the genes encoding carbapenemases of OXA40/24, KPC, VIM and NDM types were detected. Despite the fact that multidrug-resistant car-bapenem-insusceptible strains of gram-negative bacteria were isolated from a relatively small number of cancer patients, the majority of these strains represent a particular epidemiological and clinical threat.

Isolation of cultures of microorganisms from pathological material from cows

“…В результате многочисленных исследований отмечается, что причина большинства инфекционных заболеваний среди животных и людей это грамотрицательные бактерии, доминируют среди которых Enterobacteriaceae, Acinetobacter baumannii и Pseudomonas aeruginosa [4].…”

“…Live vaccines were used in the work: "FUCHSORAL" (Germany) and domestic ones -"SINRAB" (FGBU "ARRIAH, Vladimir) and its analogue -the vaccine "ORALRABIVAK" produced by OJSC "PZB" (Pokrov).In the Leningrad region and the Republic of Karelia in 2003, the FUCHSORAL vaccine (Germany) was used once, and in subsequent years, double annual vaccination was carried out with domestic vaccines. In Finland, people were constantly vaccinated with the oral rabies vaccine "FUCHSORAL" (Germany), from the "SAD B19" strain [4] We carried out annual 2-fold vaccination (in spring and autumn) of wild carnivores, in accordance with the instructions for use of vaccines, at air temperatures from +2 to +8 C in a zone 50-80 km wide from the border for 6 years. The number of blood serum samples containing a level of rabies virus-neutralizing antibodies sufficient to protect against rabies in the Republic of Karelia increased from 0% to 80%, and in the Leningrad region.…”