A practical long term structural health monitoring program must be based on reasonable field capabilities, the needs of the bridge owner, and the anticipated structural behavior unique to the structure. Each sensor installed as part of the monitoring program should provide information directly in response to operational needs related to the structure's short term and long term performance. A thoughtfully considered instrumentation plan developed in cooperation with the bridge designer, bridge manager, bridge maintenance operator and academic researchers will provide data to enhance both the state of the practice and state of the art for the bridge structural design, management and maintenance. Monitoring a bridge's structural response has the potential to (a) detect the presence of structural changes for condition assessment, (b) inform the bridge manger to assist in daily operational decision-making and (c) validate the structural design assumptions and (d) refine a structural model of the bridge to be used for performance prediction. The excitation for these responses typically comes from traffic or environmental demands. Vertical lift bridges provide a unique opportunity for structural health monitoring based on the dynamic response due to the frequent and repeated impact imparted on the structure each time the lift span opens and closes. In this paper, a structural health monitoring system designed to provide valuable information for design verification, structural model calibration, fatigue monitoring, and operational decision-making support for the reconstructed Memorial Bridge carrying US Route 1 between Portsmouth, New Hampshire and Kittery, Maine. This paper will detail the development of the sensor layout including input from stakeholders, accessibility issues and complementary and contradicting objectives. A set of structural models with varying degrees on complexity were created based on the structural performance objectives. The data collected during a pseudo-static truck load test was used to calibrate the structural models of the bridge and to select the appropriate model for each post-processing and decision-making tools related to structural performance.