Multi-scale heat and mass transfer modelling of cell and tissue cryopreservation

Xu, Feng; Moon, Sangjun; Zhang, Mengjie; Shao, Lei; Song, Young Seok; Demirci, Utkan

doi:10.1098/rsta.2009.0248

Cited by 51 publications

(44 citation statements)

References 121 publications

(164 reference statements)

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“…This droplet generation and ejection technology has been demonstrated to generate droplets at high-throughput with rates up to 1000 droplets per second [10,49,51,102–104]. Moreover, there are other droplet generation technologies that offer extremely small droplet volumes (<1 nl) based on acoustic ejection [51,105–107], which has the capability to generate smaller droplets than the methods described above.…”

Section: Vitrificationmentioning

confidence: 99%

Emerging Technologies in Medical Applications of Minimum Volume Vitrification

et al. 2011

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Cell/tissue biopreservation has broad public health and socio-economic impact affecting millions of lives. Cryopreservation technologies provide an efficient way to preserve cells and tissues targeting the clinic for applications including reproductive medicine and organ transplantation. Among these technologies, vitrification has displayed significant improvement in post-thaw cell viability and function by eliminating harmful effects of ice crystal formation compared to the traditional slow freezing methods. However, high cryoprotectant agent concentrations are required, which induces toxicity and osmotic stress to cells and tissues. It has been shown that vitrification using small sample volumes (i.e., <1 μl) significantly increases cooling rates and hence reduces the required cryoprotectant agent levels. Recently, emerging nano- and micro-scale technologies have shown potential to manipulate picoliter to nanoliter sample sizes. Therefore, the synergistic integration of nanoscale technologies with cryogenics has the potential to improve biopreservation methods.

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Section: Vitrificationmentioning

confidence: 99%

Emerging Technologies in Medical Applications of Minimum Volume Vitrification

et al. 2011

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“…Direct contact of cells with liquid nitrogen is required in both methods in order to preserve cells effectively. Adapted from Xu et al 98 FIG. 1.…”

Section: Figmentioning

confidence: 97%

“…First, cryopreservation protocols (e.g., types and concentrations of CPAs) should be optimized to decrease the risk of harmful effects on MSCs. For instance, the heat transfer modeling suggested by Xu et al 98 can be applied to optimize the current protocols in order to minimize freeze injury to MSCs due to osmotic shock.…”

Section: Conclusion and Future Perspectivesmentioning

confidence: 99%

Cryopreservation of Human Mesenchymal Stem Cells for Clinical Applications: Current Methods and Challenges

Yong

Zaman

Xufeng

et al. 2015

Biopreservation and Biobanking

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Mesenchymal stem cells (MSCs) hold many advantages over embryonic stem cells (ESCs) and other somatic cells in clinical applications. MSCs are multipotent cells with strong immunosuppressive properties. They can be harvested from various locations in the human body (e.g., bone marrow and adipose tissues). Cryopreservation represents an efficient method for the preservation and pooling of MSCs, to obtain the cell counts required for clinical applications, such as cell-based therapies and regenerative medicine. Upon cryopreservation, it is important to preserve MSCs functional properties including immunomodulatory properties and multilineage differentiation ability. Further, a biosafety evaluation of cryopreserved MSCs is essential prior to their clinical applications. However, the existing cryopreservation methods for MSCs are associated with notable limitations, leading to a need for new or improved methods to be established for a more efficient application of cryopreserved MSCs in stem cell-based therapies. We review the important parameters for cryopreservation of MSCs and the existing cryopreservation methods for MSCs. Further, we also discuss the challenges to be addressed in order to preserve MSCs effectively for clinical applications.

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“…tissue thermomechanics) and whole organ level (human pain sensation). As a demonstration of the progress in multi-scale bioheat and mass transport, Xu et al (2010) offer a review entitled 'Multi-scale heat and mass transfer modelling of cell and tissue cryopreservation', which describes a multi-scale One contribution of 9 to a Theme Issue 'Multi-scale biothermal and biomechanical behaviours of biological materials'.…”

Section: Multi-scale Biothermal and Biomechanical Behaviours Of Biolomentioning

confidence: 99%

Multi-scale biothermal and biomechanical behaviours of biological materials

2010

Phil. Trans. R. Soc. A.

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Multi-scale heat and mass transfer modelling of cell and tissue cryopreservation

Cited by 51 publications

References 121 publications

Emerging Technologies in Medical Applications of Minimum Volume Vitrification

Emerging Technologies in Medical Applications of Minimum Volume Vitrification

Cryopreservation of Human Mesenchymal Stem Cells for Clinical Applications: Current Methods and Challenges

Multi-scale biothermal and biomechanical behaviours of biological materials

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