Multi-scale magnetic nanoparticle based optomagnetic bioassay for sensitive DNA and bacteria detection

Tian, Bo; Torre, Teresa Zardán Gómez de la; Donolato, Marco; Hansen, Mikkel Fougt; Svedlindh, Peter; Strömberg, Mattias

doi:10.1039/c6ay00721j

Cited by 21 publications

(16 citation statements)

References 31 publications

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“…RCA coils produced from Vibrio cholera target DNA were investigated in the DynoMag system using 100 nm fMNPs, and the estimated theoretical limit of detection (LOD) in 200 μ l sample volume was 55 fM (femtomole/l) (Ahrentorp et al , 2016). In the opto-magnetic sensor, a LOD of 780 fM RCA coils produced from the Salmonella DNA target was achieved in a sample volume of 65 μ l (Tian et al , 2016).…”

Section: Introductionmentioning

confidence: 99%

Volume-amplified magnetic bioassay integrated with microfluidic sample handling and high-Tc SQUID magnetic readout

et al. 2017

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A bioassay based on a high- T c superconducting quantum interference device (SQUID) reading out functionalized magnetic nanoparticles (fMNPs) in a prototype microfluidic platform is presented. The target molecule recognition is based on volume amplification using padlock-probe-ligation followed by rolling circle amplification (RCA). The MNPs are functionalized with single-stranded oligonucleotides, which give a specific binding of the MNPs to the large RCA coil product, resulting in a large change in the amplitude of the imaginary part of the ac magnetic susceptibility. The RCA products from amplification of synthetic Vibrio cholera target DNA were investigated using our SQUID ac susceptibility system in microfluidic channel with an equivalent sample volume of 3 μ l. From extrapolation of the linear dependence of the SQUID signal versus concentration of the RCA coils, it is found that the projected limit of detection for our system is about 1.0 × 10 5 RCA coils (0.2 × 10 −18 mol), which is equivalent to 66 fM in the 3 μ l sample volume. This ultra-high magnetic sensitivity and integration with microfluidic sample handling are critical steps towards magnetic bioassays for rapid detection of DNA and RNA targets at the point of care.

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Section: Introductionmentioning

confidence: 99%

Volume-amplified magnetic bioassay integrated with microfluidic sample handling and high-Tc SQUID magnetic readout

et al. 2017

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“…In order to describe the trajectory of the nanoparticle in this case, one should substitute expression (17) into Eqs. (8). Using the reduced values of frequency Ω = Ω/Ω cr , we obtain…”

Section: Circularly Polarized Magnetic Fieldmentioning

confidence: 99%

“…In general case, the response of a ferrofluid to these fields is determined by the dynamics of each ferromagnetic nanoparticle included in the ferrofluid. The individual motion of the nanoparticle defines the performance of modern therapy techniques, such as targeted drug delivery [3,4] and magnetic fluid hyperthermia [3,5,6], as well as biotechnology techniques, such as biosensors, macro-molecules, and virus separation [3,7,8].…”

Section: Introductionmentioning

confidence: 99%

Dynamics and energy dissipation of a rigid dipole driven by the RF-field in a viscous fluid: Deterministic approach

Lyutyy

2018

Eur. Phys. J. E

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The deterministic rotation of a ferromagnetic nanoparticle in a fluid is considered. The heating arising from viscous friction of a nanoparticle driven by circularly and linearly polarized alternating magnetic fields is investigated. Since the power loss of such fields depends on the character of the induced motion of a nanoparticle, all types of particle trajectories are described in detail. The dependencies of the power loss on the alternating field parameters are determined. The optimal conditions for obtaining the maximum heating efficiency are discussed. The effect of heating enhancement by a static field is analyzed. The results obtained are actual for the description of heating in the magnetic fluid hyperthermia cancer treatment, when the size of the particles used is a few tens of nanometers.

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“…Ferrofluid [1,2] applications in biotechnologies and medicine are the key of stable interest to these media in recent years. Here, one should note targeted drug delivery [3,4], biosensors, macro-molecule and virus separation [3,5,6], magnetic fluid hyperthermia [3,7,8]. The latter is a promising method with principal advantages over conventional chemotherapy.…”

Section: Introductionmentioning

confidence: 99%

Uniform and nonuniform precession of a nanoparticle with finite anisotropy in a liquid: Opportunities and limitations for magnetic fluid hyperthermia

Lyutyy

Hryshko

Yakovenko

2019

Journal of Magnetism and Magnetic Materials

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We focus on an in-depth study of the forced dynamics of a ferromagnetic single-domain uniaxial nanoparticle placed in a viscous fluid and driven by an external rotating magnetic field. The process of conversion of magnetic and mechanical energies into heat is a physical basis for magnetic fluid hyperthermia that is very promising for cancer treatment. The dynamical approximation allows us to establish the limits of the heating rate and understand the logic of selection of the system parameters to optimize the therapy. Based on the developed analytical and numerical tools, we analyze from a single viewpoint the synchronous and asynchronous rotation of the nanoparticle or/and its magnetization in the following three cases. For the beginning, we actualize the features of the internal magnetic dynamics, when the nanoparticle body is supposed to be fixed. Then, we study the rotation of the whole nanoparticle, when its magnetization is supposed to be locked to the crystal lattice. And, finally, we realize the analysis of the coupled motion, when the internal magnetic dynamics is performed in the rotated nanoparticle body. In all these cases, we describe analytically the uniform mode, or synchronous rotation along with an external field, while the nonuniform mode, or asynchronous rotation, is investigated numerically.

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Multi-scale magnetic nanoparticle based optomagnetic bioassay for sensitive DNA and bacteria detection

Abstract: An optomagnetic bioassay is optimized by means of extending the linear range in the presence of larger nanoparticles.

Cited by 21 publications

References 31 publications

Volume-amplified magnetic bioassay integrated with microfluidic sample handling and high-Tc SQUID magnetic readout

Volume-amplified magnetic bioassay integrated with microfluidic sample handling and high-Tc SQUID magnetic readout

Dynamics and energy dissipation of a rigid dipole driven by the RF-field in a viscous fluid: Deterministic approach

Uniform and nonuniform precession of a nanoparticle with finite anisotropy in a liquid: Opportunities and limitations for magnetic fluid hyperthermia

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