Multi-Sensor Chip for Monitoring Key Parameters in Bioprocesses

Abstract

This paper presents a feasibility study on the integration of multiple biosensors based on amperometric, potentiometric, and impedimetric detection techniques, as well as physical sensors on a single chip. The sensor chip comprises six sensors for in-situ monitoring of important process parameters in a bioprocess: glucose, lactate, pH, cell density, dissolved oxygen concentration, and temperature. The waferlevel parallel fabrication process of multiple microsensors on a single substrate involves four relatively simple processes. The fabricated enzyme sensors were tested with a linear working range up to a concentration of 10 mM for the glucose and lactate sensors and show a fast response time of 20 s. The integrated pH sensor was governed by a near-Nernstian equation with a sensitivity of 60.8 ± 2.4 mV/pH. Cell density, dissolved oxygen, and temperature sensor were also electrically tested. Results obtained from experimental measurements can be considered a promising step towards the realization of a real-time and parallel in-situ measurement platform for bioprocesses.

Intelligent approaches for sustainable management and valorisation of food waste

“…The employment of the spectroscopy technique, however, does suffer from poor discernment, chemometrics skill, and multivariate data processing ability. Consequently, there is still a desire for durable sensor systems, low in cost, small in size, and constantly running with greater selectivity (Rusli et al, 2021). To monitor these key characteristics, many analytical approaches have been used.…”