Multi-Spectral and Fluorescence Imaging in Prevention of Overdose of Herbicides: The Case of Maize

Abstract

Evaluation of the impact of herbicides on maize was done through multispectral and multi-modal imaging and multi-spectral fluorescence imaging combined with statistical methods. Spectra containing 13 wavelengths ranging from 375 nm to 940 nm were derived from multi-spectral images in transmission, reflection and scattering mode and fluorescence images obtained using high-pass filters (F450 nm, F500 nm, F550 nm, F600 nm, F650 nm) on control maize samples and maize samples treated with Herbextra herbicide were used. The appearance of the spectra allowed us to characterize the effect of the herbicide on the maize pigment concentration. The fluorescence images allowed us to track the fate of absorbed energy and through PLS-DA and SVM-DA to discriminate the two leaf categories with very low error rates for the test, i.e. 4.9% and 2% respectively. The results of this technique can be used in the context of precision agriculture.

Development of a multispectral microscopy platform using laser diode illumination for effective and automatic label-free Plasmodium falciparum detection

“…This system was utilised as an alternative tool for staining-free malaria diagnosis. [12][13][14] The same instrument was also used to study, analyse, predict and build statistical models of infected tropical plant leaves [15][16][17] to enhance agricultural production. The potential of this optical microscope to spectrally differentiate and classify the samples' content also made it a valuable tool for biological diagnostic approaches.…”