Multi-stage laccase extraction and separation using aqueous two-phase systems: Experiment and model

Partitioning of cerrena unicolor laccase activity in an aqueous two-phase system

“…It depended on the amount of phase forming components in the mixture. Concentrations of phosphate and PEG in the respective phases were determined with the use of a mathematical model (Prinz et al, 2014), the accuracy of which was tested with ion chromatography experiments.…”

Laccase concentration by foam fractionation of Cerrena unicolor and Pleurotus sapidus culture supernatants

“…Therefore, a lot of research is focused on finding and developing novel, cost-effective, efficient methods of bioproduct separation and recovery with satisfying selectivity towards the desired product and granting its stability in the process. Various downstream processing methods of laccase have been investigated, including chromatographic methods (Scherer and Fischer, 1998) and aqueous two-phase extraction (Blatkiewicz et al, 2016;Prinz et al, 2014). One of the promising, currently investigated methods is foam fractionation (FF).…”

Hyperbranched polymers as phase forming components in aqueous two-phase extraction

“…He recognized the large potential of ATPS for the separation and purification of biomolecules and used aqueous two-phase extraction for the separation of cell organelles and proteins [5]. The industrial application of ATPS consisting of a hydrophilic salt as a phosphate and a hydrophilic polymer as polyethylene glycol has been heretofore considered for the extraction of biomolecules [6][7][8]. However, salt ions can have a salting-out effect on less polar protein molecules resulting in the lowered protein solubility in ATPS containing salts and in protein aggregation [9].…”