As high-end equipment requires a higher and higher sealing effect, sealing forms such as packing seals expose problems such as considerable wear and high heat generated by friction. Based on the theoretical analysis of the instability at the liquid-liquid interface and the pressure resistance of the seal structure, considering the rotating speed of the rotating shaft, this paper reconstructs the formula for calculating the pressure resistance value of the seal structure. On this basis, a magnetic fluid seal structure is designed for a large horizontal shaft diameter in liquid. Based on the magnetic field analysis software Maxwell, the magnetic field of the magnetic fluid seal is analyzed, and the mapping relationship between the different tooth widths of the pole teeth, the size of the permanent magnet, and the difference in the magnetic induction intensity of the seal gap is obtained. Built in the ring seal test system, the pressure test of the horizontal large shaft diameter seal structure under different rotating speeds was carried out to verify the effectiveness of the seal structure. The results have specific theoretical research significance and engineering application value for designing and applying magnetic fluid seal structures.