A novel harvesting interface for multiple piezoelectric transducers (PZTs) is proposed for high-voltage energy harvesting. Pre-biasing a PZT prior to its mechanical deformation increases its damping force, resulting in higher energy extraction. Unlike the conventional harvesters where a PZT-generated output is assumed to be continuous sinusoidal and output polarity is assumed to be alternating every cycle, PZT-generated output from human motion is expected to be random. Therefore, in the proposed approach, energy is invested to the PZT only when PZT deformation is detected. Upon the motion detection, energy stored at a storage capacitor (CSTOR) from earlier energy harvesting cycle is invested to pre-bias PZT, enhancing energy extraction. The harvested energy is transferred to back CSTOR for energy investment on the next cycle and then excess energy is transferred to the battery. In addition, partial electric charge extraction (PECE) is adapted to extract a partial amount of charges from the PZT every time its voltage approaches the process limit of 40 V. Simulations with 0.35 µm BCD process show 7.61× (with PECE only) and 8.38× (with PECE and energy investment) improvement compared to the conventional rectifier-based harvesting scheme Proposed harvesting interface successfully harvests energy from excitations with open-circuit voltages up to 100 V.