Multi-walled carbon nanotubes conjugated to tumor protein enhance the uptake of tumor antigens by human dendritic cells in vitro

Sun, Zhao; Wang, Wei; Meng, Jie; Chen, Shuchang; Xu, Haiyan; Yang, Xiao

doi:10.1038/cr.2010.133

Cited by 23 publications

(13 citation statements)

References 10 publications

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“…Healthy volunteers' blood were collected as we previously described [21]. Peripheral blood mononuclear cells (PBMC) were obtained by Ficoll-Paque density gradient centrifugation.…”

Section: Methodsmentioning

confidence: 99%

“…Our previous study revealed that CNT conjugated tumor protein could enhance the uptake of tumor antigen by human dendritic cell (DC) and the capability of DC to induce anticancer response in vitro. The results suggest that CNT-based nanotechnology may have a prospective role in the development of more efficacious DC-based anticancer immunotherapy [21]. With the expansion of the scope of prospective CNT applications, the toxicity of CNT to mammalian cells becomes an issue of great concern.…”

Section: Introductionmentioning

confidence: 99%

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Carbon Nanotubes Enhance Cytotoxicity Mediated by Human Lymphocytes In Vitro

et al. 2011

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With the expansion of the potential applications of carbon nanotubes (CNT) in biomedical fields, the toxicity and biocompatibility of CNT have become issues of growing concern. Since the immune system often mediates tissue damage during pathogenesis, it is important to explore whether CNT can trigger cytotoxicity through affecting the immune functions. In the current study, we evaluated the influence of CNT on the cytotoxicity mediated by human lymphocytes in vitro. The results showed that while CNT at low concentrations (0.001 to 0.1 µg/ml) did not cause obvious cell death or apoptosis directly, it enhanced lymphocyte-mediated cytotoxicity against multiple human cell lines. In addition, CNT increased the secretion of IFN-γ and TNF-α by the lymphocytes. CNT also upregulated the NF-κB expression in lymphocytes, and the blockage of the NF-κB pathway reduced the lymphocyte-mediated cytotoxicity triggered by CNT. These results suggest that CNT at lower concentrations may prospectively initiate an indirect cytotoxicity through affecting the function of lymphocytes.

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“…Healthy volunteers' blood were collected as we previously described [21]. Peripheral blood mononuclear cells (PBMC) were obtained by Ficoll-Paque density gradient centrifugation.…”

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Carbon Nanotubes Enhance Cytotoxicity Mediated by Human Lymphocytes In Vitro

et al. 2011

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“…The in vitro evaluation of DC-mediated anti-tumor response was performed as previously described [28]. Briefly, GO-, Ag-, or GO-Ag-pulsed DCs (1 × 10 5 /well) were co-cultured with syngeneic PBMCs (2 × 10 6 /well) in 24-well plates for 5 days in the presence of TNF-alpha (20 ng/mL, BD Pharmingen, San Jose, CA, USA).…”

Section: Methodsmentioning

confidence: 99%

In vitro enhancement of dendritic cell-mediated anti-glioma immune response by graphene oxide

Wang

Duan

et al. 2014

Nanoscale Res Lett

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Malignant glioma has extremely poor prognosis despite combination treatments with surgery, radiation, and chemotherapy. Dendritic cell (DC)-based immunotherapy may potentially serve as an adjuvant treatment of glioma, but its efficacy generally needs further improvement. Here we explored whether graphene oxide (GO) nanosheets could modulate the DC-mediated anti-glioma immune response in vitro, using the T98G human glioma cell line as the study model. Pulsing DCs with a glioma peptide antigen (Ag) generated a limited anti-glioma response compared to un-pulsed DCs. Pulsing DCs with GO alone failed to produce obvious immune modulation effects. However, stimulating DCs with a mixture of GO and Ag (GO-Ag) significantly enhanced the anti-glioma immune reaction (p < 0.05). The secretion of interferon gamma (IFN-γ) by the lymphocytes was also markedly boosted by GO-Ag. Additionally, the anti-glioma immune response induced by GO-Ag appeared to be target-specific. Furthermore, at the concentration used in this study, GO exhibited a negligible effect on the viability of the DCs. These results suggested that GO might have potential utility for boosting a DC-mediated anti-glioma immune response.

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“…Their small size (10-100 nm) is expected to not only facilitate cellular uptake [30][31][32], but also enable them to penetrate deep into tumors via enhanced permeability and retention (EPR), and even possibly induce endothelial leakiness or "nanoEL" effects [2,[33][34][35]. The nanomedicine forms of drugs have enhanced their cytotoxic effect on various types of disease, including tough-to-treat triple negative breast cancer (TNBC) [36][37][38][39]. However, the delivery of multiple drugs, attacking different facets of a cancer, in a single nanocarrier remains unexplored for TNBC.…”

Section: Introductionmentioning

confidence: 99%

Anti-migratory and increased cytotoxic effects of novel dual drug-loaded complex hybrid micelles in triple negative breast cancer cells

et al. 2015

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A polymer-based nanocarrier was developed for the co-delivery of epigenetic and chemotherapeutic drugs. The sterically stabilized hybrid micelle system uses micelles composed of D-α-tocopheryl polyethylene glycol 1000 succinate (vitamin E TPGS or TPGS) and 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[methoxy(polyethylene glycol)-2000] (DSPE-PEG2000). In this study, suberoylanilide hydroxamic acid (SAHA) and paclitaxel were used as model drugs for combination chemotherapy to enhance therapeutic efficiency in targeting mesenchyme-like triple negative breast cancer (TNBC) cells. Combination therapy of paclitaxel and SAHA in a dual drug micelle system, (P + S) mic , exhibited an IC50 value of 0.52 μg/mL, which is about 5.91-fold more cytotoxic than the mere combination of free drugs (P + S). Furthermore, the (P + S) mic formulation was far more effective at inhibiting cell migration by more than 3.4-fold than the control. Thus, our findings show that the co-delivery of these drugs using the micelle system greatly enhances their therapeutic effect at a lower dosage, thereby minimizing toxicity. In addition, this formulation is proved to be remarkably effective in preventing cell migration at low dosage.

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Multi-walled carbon nanotubes conjugated to tumor protein enhance the uptake of tumor antigens by human dendritic cells in vitro

Cited by 23 publications

References 10 publications

Carbon Nanotubes Enhance Cytotoxicity Mediated by Human Lymphocytes In Vitro

Carbon Nanotubes Enhance Cytotoxicity Mediated by Human Lymphocytes In Vitro

In vitro enhancement of dendritic cell-mediated anti-glioma immune response by graphene oxide

Anti-migratory and increased cytotoxic effects of novel dual drug-loaded complex hybrid micelles in triple negative breast cancer cells

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