Multi-walled carbon nanotubes injure the plasma membrane of macrophages

Hirano, Seishiro; Kanno, Sanae; Furuyama, Akiko

doi:10.1016/j.taap.2008.06.016

Cited by 197 publications

(139 citation statements)

References 30 publications

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“…MARCO has been shown to be a critical AM receptor for nonopsonized particles, in both rodent and human AM studies. 38,39 Using J774.1 murine macrophages, Hirano et al 40 reported that MWCNTs (of unspecified length or functionalization; 67 nm in diameter) may elicit cytotoxic effects by reacting with MARCO on the plasma membrane and rupturing the plasma membrane. Wang et al 41 showed that apoptosis in RAW 264.7 murine macrophages, induced by MWCNTs (~0.5 μm in length, 10 nm diameter; acid-treated and taurine-functionalized), could be abated by pre-treating the cells with a scavenger receptor Class A (which includes MARCO) specific neutralizing antibody.…”

Section: Discussionmentioning

confidence: 99%

Functional consequences for primary human alveolar macrophages following treatment with long, but not short, multiwalled carbon nanotubes

Sweeney¹,

Grandolfo²,

Ruenraroengsak³

et al. 2015

IJN

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Purpose: Multiwalled carbon nanotubes (MWCNTs) are a potential human health hazard, primarily via inhalation. In the lung, alveolar macrophages (AMs) provide the first line of immune cellular defense against inhaled materials. We hypothesized that, 1 and 5 days after treating AMs with short (0.6 μm in length; MWCNT-0.6 μm) and long (20 μm in length; MWCNT-20 μm) MWCNTs for 24 hours, AMs would exhibit increased markers of adverse bioreactivity (cytokine release and reactive oxygen species generation) while also having a modified functional ability (phagocytosis and migration). Methods: Primary human AMs were treated with short and long MWCNTs for 24 hours, 1 and 5 days after which toxicity end points, including cell death, reactive oxygen species generation, and inflammatory mediator release, were measured. AM functional end points involving phagocytic ability and migratory capacity were also measured. Results: AM viability was significantly decreased at 1 and 5 days after treatment with MWCNT-20 μm, while superoxide levels and inflammatory mediator release were significantly increased. At the same time, there was reduced phagocytosis and migratory capacity alongside increased expression of MARCO; this coincided with frustrated phagocytosis observed by scanning electron microscopy. In contrast, the adverse bioreactivity of the shorter MWCNT-0.6 μm with AMs (and any resulting reduction in AM functional ability) was substantially less marked or absent altogether. Conclusion:This study shows that after 24-hour treatment with long, but not short, MWCNTs, AM function is severely affected up to 5 days after the initial exposure. This has potentially significant pathophysiological consequences for individuals who may be intentionally (via therapeutic applications) or unintentionally exposed to these nanomaterials.

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Section: Discussionmentioning

confidence: 99%

Functional consequences for primary human alveolar macrophages following treatment with long, but not short, multiwalled carbon nanotubes

Sweeney¹,

Grandolfo²,

Ruenraroengsak³

et al. 2015

IJN

View full text Add to dashboard Cite

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“…These experiments found that CNTs can induce inflammation, fibrosis, and granulomas in the lungs and that CNTs have a carcinogenic effect in the abdominal cavity. In vitro experiments evaluated the effects of CNTs on lung epithelial cells, keratinocytes, and immune cells such as macrophages -5 - (Monteiro-Riviere et al, 2005;Kagan et al, 2006;Sarkar et al, 2007;Hirano et al, 2008;Jacobsen et al, 2008;Herzog et al, 2009;Yang et al, 2009). These studies found that CNTs might be cytotoxic and induce cytokine production or oxidative stress.…”

Section: Introductionmentioning

confidence: 99%

Proteomics-based safety evaluation of multi-walled carbon nanotubes

Haniu

Matsuda

Takeuchi

et al. 2010

Toxicology and Applied Pharmacology

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“…These mechanisms of MWCNTs in endothelial cells were different from the mechanisms of MWCNTs in macrophages. MWCNTs probably trigger cytotoxic effects in phagocytic cells by reacting with macrophage receptor with collagenous structure (MARCO) on the plasma membrane and rupturing the plasma membrane 40) . Taken together, the present study showed using an SD rat model that exposure to MWCNTs resulted in acceleration of development of AS.…”

Section: Discussionmentioning

confidence: 99%

Intravenous Administration of Multi‐walled Carbon Nanotubes Affects the Formation of Atherosclerosis in Sprague‐Dawley Rats

Yang

Shen

et al. 2012

Journal of Occupational Health

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Intravenous Administration of Multiwalled Carbon Nanotubes Affects the Formation of Atherosclerosis in Sprague‐Dawley Rats: Yu‐Ying XU, et al. Zhejiang University School of Medicine, China— Background Carbon nanotubes (CNTs) have many potential applications, including as delivery systems for a variety of diagnostic or therapeutic agents. However, it has been suggested that exposure to carbon nano‐materials may be a risk for the development of vascular diseases due to its impact on the vascular endothelium. Materials and Methods Male Sprague‐Dawley rats (180−200 g) were used to generate an atherosclerosis (AS) model, and the effect of intravenous administration of multi‐walled carbon nanotubes (MWCNTs) on AS was studied. To further understand the underlying mechanisms, the effects of exposure of human umbilical vascular endothelial cells (HUVECs) to MWCNTs were examined. Results Exposure to 200 μg/kg MWCNTs aggravated AS in this model. In addition, exposure to 50, 100 and 200 μg/kg MWCNTs increased the calcification of the aorta in the model. Short‐term exposure also revealed that 200 μg/kg MWCNTs injured the endothelium in the aorta. MWCNTs disrupted the endothelial tight junction and induced endothelial cell death. Conclusion The results demonstrated that MWCNTs could induce structural and functional changes in the endothelium, probably through vascular endotheliocyte injury, which eventually affected the development of AS in SD rats.

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Multi-walled carbon nanotubes injure the plasma membrane of macrophages

Cited by 197 publications

References 30 publications

Functional consequences for primary human alveolar macrophages following treatment with long, but not short, multiwalled carbon nanotubes

Functional consequences for primary human alveolar macrophages following treatment with long, but not short, multiwalled carbon nanotubes

Proteomics-based safety evaluation of multi-walled carbon nanotubes

Intravenous Administration of Multi‐walled Carbon Nanotubes Affects the Formation of Atherosclerosis in Sprague‐Dawley Rats

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