Multianalyte Pin-Printed Biosensor Arrays Based on Protein-Doped Xerogels

Abstract

We report the first biosensor arrays based on pin printing protein-doped xerogels. The individual biosensor elements are on the order of 100 microm in diameter. Arrays are formed (1) onto a planar substrate that is excited by an external source (laser) or (2) directly on the face of a light-emitting diode. We illustrate the potential of our approach by fabricating, testing, and characterizing four types of pin-printed biosensor arrays (PPBSA) for the simultaneous detection of glucose and O2. The analytically reliable operating ranges for the PPBSAs are 0.1-10 mM for glucose and 0.1-100% for O2. The PPBSAs exhibit short- and long-term reproducibilities of no worse than 4 and 8%, respectively. The overall array-to-array response reproducibilities are < or = 12%. These results demonstrate for the first time the combination sol-gel processing and pin printing methods as a way to rapidly form ensembles of integrated, reusable, and stable biosensor arrays for simultaneous multianalyte detection.

Multianalyte sensor array based on an organic light emitting diode platform

“…Multianalyte detection in a single sample using sensor arrays of various designs and sizes has been studied extensively with a range of transduction mechanisms, including electrochemical [1][2][3], piezoelectric [4], electrical resistance [5,6], and optical [7][8][9][10][11][12][13][14][15][16][17][18][19]. Such studies are driven by the need for high throughput, inexpensive, and efficient analyses of complex samples in a broad range of applications, such as medical, biological (including biodefense), environmental, and industrial (e.g., the food and beverage industry).…”

Influence of the precursor nature and deposition mode on the oxygen sensing properties of Ru(II) complex immobilized in a SiO2-based matrix

“…2(b) and (c)) and dissolved ( Fig. 4) O 2 measurements are rather good fits to the Stern-Volmer equation, as opposed to, for example, the data reported by Bright et al [24,25] which follow the Demas two-site model [21].…”

CMOS-Based Phase Fluorometric Oxygen Sensor System

“…Overall, the plot shows good reproducibility. Finally, our previous analysis of these xerogel based sensors elements indicate that they are stable (reproducibility of better than 8%) for at least a year [10], [12], [37].…”