Multicenter evaluation of an amperometric immunosensor for plasma fatty acid-binding protein:

Key, Göran; Schreiber, Andrea; Feldbrügge, Rainer; McNeil, Calum J.; Jørgensen, Per Schultz; Pelsers, Maurice M.A.L.; Glatz, Jan F. C.; Spener, Friedrich

doi:10.1016/s0009-9120(98)00108-8

Cited by 25 publications

(6 citation statements)

References 8 publications

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“…Although B-FABP and H-FABP appear to be rapidly released into the circulation, early detection of acute brain injury such as in stroke would allow the early initiation of therapy only when rapid detection systems are available. For H-FABP, rapid assays are already available, such as a microparticle-enhanced immunoassay (41 ), an amperometric enzyme immunosensor (42 ), and lateral-flow assays (43 ). In addition, new developments in online measurement of FABP (44 ) could enable rapid detection of brain injury and/or changes in permeability of the bloodbrain barrier attributable to reduced blood flow, for example, during cardiac by-pass surgery (7 ).…”

Section: Discussionmentioning

confidence: 99%

Brain- and Heart-Type Fatty Acid-Binding Proteins in the Brain: Tissue Distribution and Clinical Utility

Pelsers¹,

Hanhoff

Voort³

et al. 2004

Clinical Chemistry

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109

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Section: Discussionmentioning

confidence: 99%

Brain- and Heart-Type Fatty Acid-Binding Proteins in the Brain: Tissue Distribution and Clinical Utility

Pelsers¹,

Hanhoff

Voort³

et al. 2004

Clinical Chemistry

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109

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“…28 With prefabricated, screen-printed electrodes, an assay time of 20 min with a measuring range of 10-350 ng/mL was achieved, covering the pathological range of H-FABP in the circulation and exhibiting good correlation with the reference sandwich ELISA. 29 Here we demonstrate that the competitive assay format with its reduced number of incubations can also be employed to develop fast immunoassays, even in the popular microtiter format.…”

mentioning

confidence: 90%

A Genetically Engineered Fusion Protein with Horseradish Peroxidase as a Marker Enzyme for Use in Competitive Immunoassays

et al. 2001

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Horseradish peroxidase is one of the most widely used marker enzymes in immunoassays. Several disadvantages are encountered upon chemical conjugation of peroxidase with antibodies or antigens, as are low reproducibility and undefined stoichiometry. We here describe for the first time the production of a recombinant fusion of a protein analyte with horseradish peroxidase in Escherichia coli, employing refolding of inclusion bodies and reconstitution with heme. The genetic fusion approach enables preparation of conjugates with 1:1 stoichiometry and defined structure. As a protein analyte, the human heart fatty acid binding protein (H-FABP) was chosen, which is a new and sensitive marker for acute myocardial infarction. The recombinant conjugate was fully active [650 U/mg with 2,2-azino-bis(3-ethyl-thiazoline-6-sulfonate) as substrate] and obtained in a yield of 12 mg/L of E. coli culture, which is better than that for recombinant peroxidase alone. The competitive immunoassay that was developed with the recombinant conjugate requires fewer incubation steps than the traditional sandwich ELISA format. It permitted the detection of H-FABP directly in plasma in the range of 10-1500 ng/mL which is the relevant range for clinical decision-making.

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“…The obtained results demonstrated that the immunosensor could be helpful for fast clinical decisions which could be run on‐site. However, for low concentration ranges (5–30 µg L −1 ) the sensor tended to overestimate 29. The same sandwich configuration was used by O’Regan et al.…”

Section: Electrochemical Biosensors For Cardiovascular Markersmentioning

confidence: 99%

Electrochemical Biosensors for the Determination of Cardiovascular Markers: a Review

2014

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Nowadays there is a growing interest in the development of devices for the fast and reliable detection and quantification of biomarkers associated with cardiovascular diseases (CVDs). The main objective is not only an early diagnosis of these diseases but also predicting the risk that an individual can suffer CVD. Electrochemical biosensors can play a key role in the development of low cost, portable and rapid sensing platforms for this purpose. This review discusses critically the state of the art in the development, applications and potential suitability of electrochemical biosensors for the determination of cardiovascular biomarkers as well as the required characteristics that these sensors should fulfill to be finally accepted in the clinical community.

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Multicenter evaluation of an amperometric immunosensor for plasma fatty acid-binding protein:

Cited by 25 publications

References 8 publications

Brain- and Heart-Type Fatty Acid-Binding Proteins in the Brain: Tissue Distribution and Clinical Utility

Brain- and Heart-Type Fatty Acid-Binding Proteins in the Brain: Tissue Distribution and Clinical Utility

A Genetically Engineered Fusion Protein with Horseradish Peroxidase as a Marker Enzyme for Use in Competitive Immunoassays

Electrochemical Biosensors for the Determination of Cardiovascular Markers: a Review

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