Multicenter evaluation of new enzyme-linked immunoassays of follitropin and lutropin in serum or plasma

Abstract

Results from a multicenter evaluation of two new enzyme-linked immunosorbent assays [Enzymun-Test for follitropin (FSH) and lutropin (LH)] are presented and compared with results from 11 other commercial immunoassays, radioactive as well as nonradioactive. Enzymun-Test FSH and LH assays are suitable for automated systems and manual applications. The tests were reproducible (CV less than 5%), highly specific, and sensitive enough (less than 0.5 int. unit/L) to measure the hormones directly in almost all patients' samples, except for LH measurements in prepubertal children. We did not find interference by heterophilic antibodies or other factors. A comparison of assays for FSH found very good agreement among all modern two-site assays; competitive immunoassays almost invariably yielded systematically lower results for FSH, probably because of the heterogeneity of the International Reference Preparation (2nd IRP FSH, 78/549). For LH also we found good agreement, with no systematic differences among the various reagents. Guidelines for reference values with the new reagents are given.

Morphometric and hormonal changes during the Chimpanzee menstrual cycle

“…Samples were run in duplicate and in one run. The specific antibodycharacteristics and cross-sera used have been validated for humans [26,31,50,59,63]. PrecinormÒ IM and PrecipathÒ IM (Boehringer Mannheim) were used as quality control sera.…”

Role of transient hyperprolactinemia in the late follicular phase of the gonadotropin‐stimulated cycle

“…The serum PRL concentration was determined by using an enzyme-linked immunosorbent assay (ELISA; ES700 system, Enzymun-Test; Boehringer-Mannheim Co., Tokyo, Japan; conversion factor to ng/mL in immunoradiometric assay (IRMA) = 1/45). 22 The serum E 2 concentration was determined by using an enzyme immunoassay (EIA; AIA-PACK E2 system; TOSOH Co., Tokyo, Japan), 23 and the E 2 concentration in the incubation medium was determined by using a RIA. 24…”