Multicenter evaluation of proposed standardized procedure for antifungal susceptibility testing of filamentous fungi

Espinel-Ingroff, Ana; Bartlett, Marilyn S.; Bowden, Raleigh A.; Chin, Nai-Xun; Cooper, Chester R.; Fothergill, A.; McGinnis, Michael R.; Menezes, Paulo H.; Messer, S. A.; Nelson, Page W.; Odds, Frank C.; Pasarell, L.; Peter, Joanne; Pfaller, Michael A.; Rex, John H.; Rinaldi, Michael G.; Shankland, G. S.; Walsh, Thomas J.; Weitzman, Irene

doi:10.1128/jcm.35.1.139-143.1997

Cited by 203 publications

(93 citation statements)

References 17 publications

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“…82 A study of 11 centers with 30 organisms of Aspergillus sp, Fusarium sp, Rhizopus sp, and Pseudallescheria boydii was conducted to evaluate proposed guidelines for reference broth microdilution testing. 83 This study evaluated conventional broth microdilution and colorimetry with Alamar Blue for testing amphotericin B and itraconazole. Optimal conditions for the drugs were determined to be a starting inoculum of 1 x 10 4 cfu/ml, RPMI 1640 broth medium, and an incubation time of 48-72 hours at 35°C.…”

Section: Cryptococcus Neoformansmentioning

confidence: 99%

In Vitro Antifungal Susceptibility Testing

Hoffman

Pfaller

2001

Pharmacotherapy

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With the rising frequency of fungal infections, as well as increasing reports of resistance to antifungal agents, it is imperative that clinically applicable antifungal susceptibility testing be available. In 1997 the National Committee for Clinical Laboratory Standards published standard guidelines for antifungal susceptibility testing of Candida sp and Cryptococcus neoformans with amphotericin B, flucytosine, fluconazole, itraconazole, and ketoconazole. Although the methods are standard, they are time consuming, can be difficult to interpret, and are approved only for testing limited organisms and drugs. Modifications to the methods and alternative approaches have been proposed to make these tests more convenient and efficient, applicable to a greater number of species, and appropriate for performing in the clinical laboratory.

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Section: Cryptococcus Neoformansmentioning

confidence: 99%

In Vitro Antifungal Susceptibility Testing

Hoffman

Pfaller

2001

Pharmacotherapy

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“…NCCLS microdilution assay -The in vitro effectiveness of PACT therapy was tested in a modified version of the NCCLS microdilution assay, as described previously by Espinel-Ingroff et al 9 In summary, 10.6 9 10 5 CFU/100 lL of T. rubrum were incubated with 100 lL of TBO, final concentration 50 lg/mL, 50 lL of TBO, final concentration 25 lg/mL, or PBS, respectively, for 1 min. Then, the 5 mm thick CFU-suspension layer was irradiated with the PACT LED, 635 nm wavelength and 500 mW, 200 J/cm 2 for 0, 5, 10 and 20 min.…”

Section: Methodsmentioning

confidence: 99%

Efficacy of antifungal PACT in an in vitro model of onychomycosis

Mehra

Schaller

Walker

et al. 2014

Acad Dermatol Venereol

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“…Antifungal agents used here were selected according to previous reports on the control of Aspergillus spp. by Espinel-Ingroff et al (1997) and Provine and Hadley (2000). Amphotericin B was initially dissolved in dimethyl sulfoxide, while itraconazole was dissolved in 0AE5 ml of chloroform.…”

Section: Antifungal Agentsmentioning

confidence: 99%

Modulation of water activity on fungicide effect on Aspergillus niger growth in Sabouraud dextrose agar medium*

Streett

2005

Lett Appl Microbiol

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X . N I A N D D . A . S T R E E T T . 2005.Aims: To examine whether water activity (a w ) in combination with low concentration of fungicides can be used to effectively control Aspergillus niger van Tieghem growth in cultural medium, the Sabouraud dextrose agar (SDA). The data would be used as baseline information for reducing A. niger contamination in insect artificial diets. Methods and Results: Aspergillus niger was isolated from an insect artificial diet. Four concentration levels (i.e. 0, 1, 10 and 20 lmol) of two fungicides (i.e. amphotericin B and itraconazole) were tested against A. niger under four a w levels (i.e. 0AE994, 0AE961, 0AE921 and 0AE859) adjusted by including 0, 12AE5, 25 and 38% of glycerol in the medium mixture. Aspergillus niger growth was significantly reduced at low fungicide concentration (1 lmol), and at reduced a w . The spore germination was prevented with either higher fungicide concentration (>10 lmol), or low a w in the medium (a w < 0AE921). The two ecological determinants (fungicides and a w ) showed a significant impact on A. niger survival in the medium (P < 0AE0001). Itraconazole is more effective than amphotericin B in controlling A. niger contamination in the agar medium. Conclusion: Adjustment of a w (with 12AE5% of glycerol) in combination with 1 lmol of itraconazole can effectively prevent A. niger growth in the SDA cultural medium. Significance and Impact of the Study: Aspergillus niger contaminations have frequently affected the quality of insects produced from mass rearing facilities. Low a w in combination with low fungicide concentration has the potential to become one of the most cost-effective management strategies to prevent A. niger contamination in insect artificial diets. The effect of fungicides and low a w in artificial diets on insect biology needs to be further examined.

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Multicenter evaluation of proposed standardized procedure for antifungal susceptibility testing of filamentous fungi

Cited by 203 publications

References 17 publications

In Vitro Antifungal Susceptibility Testing

In Vitro Antifungal Susceptibility Testing

Efficacy of antifungal PACT in an in vitro model of onychomycosis

Modulation of water activity on fungicide effect on Aspergillus niger growth in Sabouraud dextrose agar medium*

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