Multicenter Evaluation of the Vitek MS Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry System for Identification of Gram-Positive Aerobic Bacteria

Rychert, Jenna; Burnham, Carey‐Ann D.; Bythrow, Maureen; Garner, Omai B.; Ginocchio, Christine C.; Jennemann, Rebecca; Lewinski, Michael A.; Manji, Ryhana; Mochon, A. Brian; Procop, Gary W.; Richter, Sandra; Sercia, Linda; Westblade, Lars F.; Ferraro, Mary Jane; Branda, John A.

doi:10.1128/jcm.00682-13

Cited by 121 publications

(67 citation statements)

References 42 publications

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“…Few laboratories routinely include anaerobic media or culture conditions as part of the workup for CSF specimens in light of the fact that anaerobes are not the organisms classically associated with acute bacterial meningitis. Our patient's P. acnes isolate was initially cultured anaerobically on brucella blood agar, and its identification was later confirmed using two matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) mass spectrometry (MS) platforms, the Bruker Biotyper (software version 3.0; Bruker Daltonics, Billerica, MA) and Vitek MS (database version 2.0; bioMérieux) (23,24). Although details of culture methods are not described in all previous case reports, broth me- dium, such as thioglycolate or Robertson's chopped meat broth, and cysteine Trypticase semisolid agar were used to isolate P. acnes from CSF specimens (25).…”

Section: Case Reportmentioning

confidence: 99%

De Novo Meningitis Caused by Propionibacterium acnes in a Patient with Metastatic Melanoma

et al. 2014

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Propionibacterium acnes is a known cause of postneurosurgical meningitis; however, it is rarely implicated in de novo meningitis. Herein we report a case of a 49-year-old male with de novo meningitis caused by P. acnes with metastatic melanoma as the only identified risk factor for his infection. CASE REPORTA 49-year-old Caucasian male presented to his primary care physician with an 11-week history of bifrontal headaches associated with nausea and emesis but without other symptoms of meningismus. Symptomatic relief was provided with over-thecounter pain medications and antiemetics. Five weeks prior to arrival at our institution, the patient was admitted to an outside hospital for intractable vomiting and headaches. A computed tomography (CT) scan of the head and abdomen were performed, both of which were normal. Two weeks prior to admission, his headache and nausea worsened with the onset of subjective fevers, low back pain, lethargy, and confusion.Roughly 2 years prior to admission, the patient was diagnosed with stage IIIC melanoma of the right lower extremity, for which he underwent resection and lymphadenectomy. In the intervening period, he was thought to be in remission. Eight weeks prior to admission, a left-lower-extremity skin biopsy specimen demonstrated a melanocytic nevus.At the time of admission, his vital signs were as follows: temperature, 36.7°C; blood pressure, 168/92 mm Hg; heart rate, 87 beats per minute; respiratory rate, 20 breaths per minute; oxygen saturation of 98% on room air; height, 188 cm (74 in.); and weight, 142 kg (312.4 lb). The physical exam showed a well-developed male in no acute distress, alert, and oriented to place, year, self, and situation. Notable findings included right-lower-extremity swelling, a healed scar at the site of his prior melanoma, and ecchymosis surrounding a left-lower-extremity biopsy site. He did not have signs of meningismus.Laboratory findings on admission included a hemoglobin level of 14.1 g/dl, hematocrit of 40.3%, white blood cell count of 10,300/mm 3 , mildly elevated absolute neutrophil count of 7,600/ mm 3 (range, 1,800 to 6,600/mm 3 ), normal chemistry panel, an alanine transaminase level of 89 U/liter (range, 7 to 53 U/liter) but otherwise normal hepatic function panel, and a normal urinalysis.A repeat CT scan of the head was obtained on hospital day 2, 5 weeks after the prior scan, which revealed a communicating hydrocephalus. CT myelography was also performed on day 2, which showed clumping of the nerve roots within the lumbar spine and nodular thickening surrounding L2-L3 nerve roots consistent with arachnoiditis versus metastatic disease.A fluoroscopically guided lumbar puncture (LP) was performed on hospital day 2, which yielded clear, yellow cerebrospinal fluid (CSF) with a glucose concentration of 26 mg/dl (serum glucose, 96 mg/dl), protein concentration of 518 mg/dl, 145 nucleated cells/l (1% neutrophils, 32% macrophages, 10% monocytes, 6% lymphocytes, and 51% unclassified cells due to poor cell viability), 576 red blood cells/l, and an...

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Section: Case Reportmentioning

confidence: 99%

De Novo Meningitis Caused by Propionibacterium acnes in a Patient with Metastatic Melanoma

et al. 2014

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“…The VITEK MS identification system is based on comparison of the characteristics of the obtained spectra with those of the VITEK MS v2.0 database (7,8). All of the statistical analyses were performed with SPSS 19.0.…”

Section: Discussionmentioning

confidence: 99%

“…The system reported the best identification with confidence values from 0 to 99.9%. Escherichia coli ATCC 8739 was used as internal identification control during the whole analysis process (7,8).…”

Section: Maldi-tof Mass Spectrometrymentioning

confidence: 99%

Matrix-assisted laser desorption ionization time-of-flight mass spectrometry: a powerful tool for identification of Corynebacterium species

Bao¹,

Gao²,

Hu³

et al. 2017

J. Thorac. Dis.

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“…Branda et al evaluated the VITEK MS system for identification of 226 isolates of fastidious gram-negative bacteria; 97% and 96% of the fastidious gram-negative bacteria studied were accurately identified to the genus and species levels, respectively (22 ). Rychert et al evaluated the Vitek MS system for identification of 1146 aerobic gram-positive bacteria and showed that 93% were correctly identified to the species level, with an additional 3% identified only to the genus level because of a split identification with multiple matching species within the same genus (including Listeria monocytogenes with matches to L. monocytogenes, Listeria ivanovii, and Listeria welshimeri) (23 ). Karpanoja et al compared the MALDI Biotyper and VITEK MS IVD systems for identification of viridans group streptococci using 54 type strains and 97 blood culture isolates (24 ).…”

Section: Performance Of Maldi-tof Ms For Identification Of Aerobic Bamentioning

confidence: 99%

MALDI-TOF MS for the Diagnosis of Infectious Diseases

2015

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BACKGROUND:First introduced into clinical microbiology laboratories in Europe, MALDI-TOF MS is being rapidly embraced by laboratories around the globe. Although it has multiple applications, its widespread adoption in clinical microbiology relates to its use as an inexpensive, easy, fast, and accurate method for identification of grown bacteria and fungi based on automated analysis of the mass distribution of bacterial proteins.CONTENT: This review provides a historical perspective on this new technology. Modern applications in the clinical microbiology laboratory are reviewed with a focus on the most recent publications in the field. Identification of aerobic and anaerobic bacteria, mycobacteria, and fungi are discussed, as are applications for testing urine and positive blood culture bottles. The strengths and limitations of MALDI-TOF MS applications in clinical microbiology are also addressed.

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Multicenter Evaluation of the Vitek MS Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry System for Identification of Gram-Positive Aerobic Bacteria

Cited by 121 publications

References 42 publications

De Novo Meningitis Caused by Propionibacterium acnes in a Patient with Metastatic Melanoma

De Novo Meningitis Caused by Propionibacterium acnes in a Patient with Metastatic Melanoma

Matrix-assisted laser desorption ionization time-of-flight mass spectrometry: a powerful tool for identification of Corynebacterium species

MALDI-TOF MS for the Diagnosis of Infectious Diseases

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