2006
DOI: 10.1128/jcm.44.3.688-692.2006
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Multicenter Laboratory Validation of the BACTEC MGIT 960 Technique for Testing Susceptibilities of Mycobacterium tuberculosis to Classical Second-Line Drugs and Newer Antimicrobials

Abstract: The BACTEC MGIT 960 system, a fully automated, nonradiometric, noninvasive system for detection and drug susceptibility testing of mycobacteria, was evaluated for the ability to test susceptibilities to second-line drugs. In this study, which was carried out in three phases (phase I, mostly susceptible strains; phase II, mostly resistant strains; phase III, final testing of the optimal drug concentrations found in phases I and II), we established the critical concentrations for seven drugs to be tested in the … Show more

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Cited by 189 publications
(144 citation statements)
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“…The drug susceptibility of every successful isolate of M. tuberculosis was then investigated at the National TB Reference Laboratory by using BACTEC MGIT 960 supplemented with isoniazid (0.1 μg/ml), rifampicin (1.0 μg/ml), ethambutol (5.0 μg/ml) or streptomycin (1.0 μg/ml). The isolates found to be multidrug-resistant were then tested for resistance to second-line anti-TB drugs 10,11 with BACTEC MGIT 960 supplemented with kanamycin (2.5 μg/ ml), amikacin (1.0 μg/ml), capreomycin (2.5 μg/ml) or ofloxacin (2.0 μg/ml). An MDR-TB isolate that showed resistance to ofloxacin and at least one of the injectable drugs used for second-line treatment (i.e.…”
Section: Laboratory Methodsmentioning
confidence: 99%
“…The drug susceptibility of every successful isolate of M. tuberculosis was then investigated at the National TB Reference Laboratory by using BACTEC MGIT 960 supplemented with isoniazid (0.1 μg/ml), rifampicin (1.0 μg/ml), ethambutol (5.0 μg/ml) or streptomycin (1.0 μg/ml). The isolates found to be multidrug-resistant were then tested for resistance to second-line anti-TB drugs 10,11 with BACTEC MGIT 960 supplemented with kanamycin (2.5 μg/ ml), amikacin (1.0 μg/ml), capreomycin (2.5 μg/ml) or ofloxacin (2.0 μg/ml). An MDR-TB isolate that showed resistance to ofloxacin and at least one of the injectable drugs used for second-line treatment (i.e.…”
Section: Laboratory Methodsmentioning
confidence: 99%
“…Among the commercially available methods described above, the MGIT system, both in its manual and automated versions, have shown high sensitivity and specificity in detecting resistance to RIF and INH, the two most important drugs in the treatment of TB. DST to other firstand second-line drugs have also shown good accuracy, although additional studies are needed for second-line drugs to determine the optimal critical concentrations to be tested (Rusch-Gerdes et al, 2006). More studies are also needed to assess the performance of MGIT for DST of PZA and for performing DST directly on sputum samples.…”
Section: Future Perspectivesmentioning
confidence: 99%
“…A recent meta-analysis of the published studies found high accuracy and high predictive values associated with the use of BACTEC MGIT960 (Piersimoni et al, 2006). Also, a multicentre validation has been performed to test susceptibilities of M. tuberculosis to classical second-line drugs giving accurate results as compared to the BACTEC TB-460 radiometric method (Rusch-Gerdes et al, 2006). The BACTEC MGIT960 system is now practically replacing the radiometric BACTEC TB-460 as a rapid and reliable method for diagnosis and DST of M. tuberculosis.…”
Section: The Bactec Mgit960 Automated Systemmentioning
confidence: 99%
“…Resistance to the four primary drugs-INH, SM, EMB and RIF-makes tuberculosis difficult to treat. The emergence of MDR-TB within the last decade has posed an exceptionally severe threat and underscores the significance of rapid antimicrobial susceptibility testing of MDR-TB strains to effectively treat patients [4][5][6].…”
Section: Introductionmentioning
confidence: 99%
“…Recently, the nonradiometric, fully automated Bactec MGIT 960 system (MGIT) (Becton Dickinson, Sparks, MD) technology has provided a new step forward, as it allows continuous monitoring of positive fluorescence, which is based on bacterial growth. This MGIT system has been proposed as a method for determining susceptibility of M. tuberculosis strains to the first-line antituberculosis drugs: INH, RIF, EMB and SM [4][5][6][8][9][10].…”
Section: Introductionmentioning
confidence: 99%