Multicenter study of a new fully automated HBsAg screening assay with enhanced sensitivity for the detection of HBV mutants

Mühlbacher, Annelies; Weber, Bernard; Bürgisser, Philippe; Eiras, A.; Cabrera, Javier; Louisirirotchanakul, Suda; Tiller, Friedrich-Wilhelm; Kim, Hak-Sub; Helden, Josef van; Bossi, V.; Echevarría, José Manuel

doi:10.1007/s00430-007-0059-9

Cited by 57 publications

(47 citation statements)

References 28 publications

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“…Upstream (between 164 and 215) of MHR region of native and or recombinant surfance proteins has different binding capcities to antibodies. [9][10][11] Hence, the classical definition of the 'a' immunodominant region may need to be extended to require adjacent amino acids supporting its conformation. The amino acid sequence of the polymerase between aa163 and aa210 is the region that can confer resistance to some polymerase inhibitors, when mutated.…”

Section: Discussionmentioning

confidence: 99%

A new vaccine escape mutant of hepatitis B virus causes occult infection

Shang

2015

Human Vaccines & Immunotherapeutics

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There is growing public concern regarding assay sensitivity to HBsAg mutants in clinical diagnosis and vaccine escape. The aim of this study is to introduce a new HBsAg mutant strain. The serum samples were those of patient X at the age of 3 months and 3 years respectively, and of her mother immediately before parturition, which were used to amplify the HBsAg-coding DNA fragments by PCR. The HBsAg DNA sequences were translated into their corresponding amino acid sequences and then aligned in pubmed with nucleotide blast. The sequencing data of S coding regions shows that patient X has been infected by a new HBV variant with an A to C substitution at nt431, resulting in an Asp (GAC)to Ala(GCC) substitution at aa144 of major protein; CC to AA substitution at nt359 and nt360, resulting in an Pro (CCC) to Gln(CAA) substitution at aa120 of pre "a" epitope; A to G substitution at nt491, resulting in an Glu(GAG) to Gly (GGG) substitution at aa164 of post "a" epitope. Three new mutations (S171F, S174N and Q181R) at the antigenic epitopes of HBV presented by HLA class I molecules are found. The HBV mutant strain causes vaccine escape and occult infection.

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Section: Discussionmentioning

confidence: 99%

A new vaccine escape mutant of hepatitis B virus causes occult infection

Shang

2015

Human Vaccines & Immunotherapeutics

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“…HBs Ag and HCV Ab+ were detected by NIBSC 00/588; WHO 2nd International Standard for HBsAg, subtype adw2, genotype A; UI/mL with 99.9% specificity and 99.8% sensitivity (Muhlbacher et al, 2008) and hepatitis C antibody (Anti-HCV) by cobas e 411 analyzer, Elecsys® 2010 analyzer: 4.2 -5.2 % with100.0% sensitivity and 99.6% hospitalized patients clinical specificity (Esteban, 2013 ) …”

Section: Laboratory Methodsmentioning

confidence: 99%

The associated factors of HBV and/or HCV with HIV co-infection among men who have sex with men (MSM) in the Northern region of Thailand, 2015

Wongwichit¹,

Singharachai²,

Suwanaporn³

et al. 2017

jmms

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Men who have sex with men (MSM) are at higher risk for co-infection with hepatitis B virus (HBV), hepatitis C virus (HCV) than the general population. The co-infection accelerates disease progressionreciprocally. This study purposed to investigate the prevalence and the associated factors with HBV and/or HCV co-infection with HIV among MSM in Northern region Thailand in 2015. A case-control study design was conducted by a hospital-based in anti-retroviral (ARV) clinic. 31 of 51 hospitals in the Phayao, Chiang Mai and Chiang Rai province were recruited in this study. The result was found 374 total participants who were registered patients in ARV clinic inPhayao84 cases (22.5 %), Chiang Mai 170 (45.5%) and Chiang Rai120 (32.1%). The participants were 30-43 year old (47.1%), followed by 16-29 years old (29.9%), and 44-57 years old (19.8%). The majority of occupational were employee (50.0%), follow by merchant (24.9%) and agriculturist (9.4%).The univariate analysis with a significant α level of 0.05were found six factors had significantly association with HBV and/or HCV with HIV co-infection including (1) age range (2) smoking behavior (3) tongue piercing, (4) Stavudine drug taking, (5) Tenofivir drug taking and (6) Nevirapine drug taking. Moreover, the binary logistic regression at a significant α level of 0.05, after controlling for all possible confounding factors, were found four factors significantly association with co-infection including (1) age between 30-43 year old had the risk of co-infection 2.42 times [odds ratio (OR) = 2.42, 95% confidence interval (CI) = 1.14-5.14] higher than those who had age range 16-29 year old, (2) smoking behavior in the participants had the risk of co-infection 2.05 times (OR = 2.05, 95% CI = 1.12-3.76) higher than no smoking behavior. (3)Tongue piercing in the participants had the risk of co-infection 6.82 times (OR = 6.70, 95% CI = 1.12-40.19) higher than no tongue piercing and (4) Tenofivir drug taking had the risk of co-infection 4.30 (OR = 4.30, 95% CI = 2.37-7.84) more than no taking drug. Therefore, enhancing the surveillance and targeted prevention of viral hepatitis including improved disease prevention, vaccination coverage, sanitation and food safety, safer sex and efficiency healthcare delivery,

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“…Also, HBV detection methods differ in developed and developing countries according to the level of endemic HBV infections. Besides, automated HBsAg detection immunoassay systems have been developed and widely used [6][7][8][9]. However, high specificity enzyme-linked immunosorbent assays (ELISAs) are still commonly used [4,5,10,11].…”

Section: Article; Medical Biotechnology (Mb)mentioning

confidence: 99%

Cloning of anti-HBsAg single-chain variable fragments from hybridoma cells for one-step ELISA

Erdağ

Balcıoğlu

Bahadır

et al. 2017

Biotechnology & Biotechnological Equipment

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Hepatitis B virus (HBV) infection is a worldwide health problem. More than 400 million people are chronic HBV carriers in the world. Infected individuals are at a high risk of developing liver cirrhosis and hepatocellular carcinoma as the main consequences of HBV. The discoveries of fast diagnostic systems and new therapeutic applications are crucial in the fight against viral hepatitis. In this paper we present the generation of a single-chain variable fragment (scFv) from a mouse monoclonal antibody specific to the HBV surface antigen (HBsAg) and demonstrate its expression as a bacterial alkaline phosphatase (AP) fusion protein. In this study, we constructed scFvs from hybridoma cells expressing HBsAg-specific antibody using phage display technology and expressed them in Escherichia coli. The anti-HBsAg scFvs were inserted into pQE-2 vector to produce scFv antibody genetically fused to bacterial AP. Reproducibility of the recombinant HBsAg-scFv fusion protein was tested using Enzyme-linked Immunosorbent Assay (ELISA). Present preliminary findings indicate that the anti-HBsAg-scFv AP conjugate could be further used for the development of one-step ELISA for the detection of HBV.

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Multicenter study of a new fully automated HBsAg screening assay with enhanced sensitivity for the detection of HBV mutants

Cited by 57 publications

References 28 publications

A new vaccine escape mutant of hepatitis B virus causes occult infection

A new vaccine escape mutant of hepatitis B virus causes occult infection

The associated factors of HBV and/or HCV with HIV co-infection among men who have sex with men (MSM) in the Northern region of Thailand, 2015

Cloning of anti-HBsAg single-chain variable fragments from hybridoma cells for one-step ELISA

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