Multicenter study of clinical non-β-lactam-antibiotic susceptible MRSA strains: Genetic lineages and Panton-Valentine leukocidin (PVL) production

Ceballos, Sara; Aspíroz, Carmen; Ruiz-Ripa, Laura; Azcona-Gutiérrez, Jose Manuel; López-Cerero, Lorena; López-Calleja, Ana Isabel; Álvarez, Ledicia; Gomariz, María José Barahona; Fernández, María Concepción López; Torres, Carmén; Ezpeleta, Carmen; Martin, C. J.; Arribas, Jorge; Navarro, Carmen; Arias, Antonina; Fortuño, B; Pereira, Javier; Milagro, Ana; Torres, Luís; Soria-Blanco, Luis Miguel; Canut, Andrés; Cordón, Ma Luz; Megías, Gregoria; Calvo, Jorge; Rezusta, Antonio

doi:10.1016/j.eimc.2019.01.015

Cited by 8 publications

(4 citation statements)

References 31 publications

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“…This CC is known to be a livestock‐lineage mostly related with ruminants but also found in wild animals, and the environment (García‐Álvarez et al ; Gómez et al ; Gómez et al ). The presence of the mecC gene in clinical isolates in Spain is still unusual, as evidenced by a recent multicentre study conducted in 12 Spanish hospitals among non‐β‐lactam‐susceptible MRSA isolates (common phenotype of mec C strains) (Ceballos et al ). Nevertheless, sporadic detections of clinical mec C MRSA strains have been reported in Spain, corresponding to the lineage t843 and ST130/ST1945 (included in CC130) (Romero‐Gómez et al ; García‐Garrote et al ; Cano‐García et al ; Benito et al ), similar to the mecC ‐MRSA isolates detected in this study.…”

Section: Discussionmentioning

confidence: 99%

Diversity ofStaphylococcus aureusclones in wild mammals in Aragon, Spain, with detection of MRSA ST130‐mecCin wild rabbits

et al. 2019

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Aims To determine the Staphylococcus aureus carriage rate in wild mammals in Aragon, northern Spain, to analyse their antimicrobial resistance phenotype/genotype and to characterize the recovered isolates. Methods and Results Nasal and rectal swabs of 103 mammals were collected in Aragón during the period 2012–2015. Antimicrobial susceptibility, the presence of antimicrobial resistance genes and virulence factors were investigated. Molecular characterization was carried out by spa, MLST, agr and SCCmec. Staphylococcus aureus were recovered from 23 animals (22%). Four of the 23 S. aureus were methicillin‐resistant S. aureus (MRSA). Three MRSA were mecC‐positive and were isolated from European rabbits and were typed as t843 (ascribed to CC130). The remaining MRSA was a mecA‐carrying isolate from European hedgehog, typed as ST1‐t386‐SCCmecIVa‐agrIII and it harboured the blaZ, erm(C), ant(6)‐Ia and aph(3´)‐IIIa resistance genes. A high diversity of spa‐types was detected among the 19 methicillin‐susceptible S. aureus isolates, which showed high susceptibility to the antimicrobials tested. The tst gene and different combinations of staphylococcal enterotoxins were found. Conclusions Staphylococcus aureus were detected in nasal and rectal samples of wild mammals. Wild rabbits could be a reservoir of mecC‐MRSA. Significance and Impact of the Study This work provides information on the presence and characteristics of S. aureus from mammals in a defined geographic region in Spain.

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Section: Discussionmentioning

confidence: 99%

Diversity ofStaphylococcus aureusclones in wild mammals in Aragon, Spain, with detection of MRSA ST130‐mecCin wild rabbits

et al. 2019

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“…While all S. aureus isolates were collected in some studies [ 29 , 38 , 45 ], only MRSA isolates were included in others [ 24 , 26 , 27 , 28 , 32 , 33 , 36 , 41 , 42 ]. Moreover, several studies were more restrictive and only used isolates that showed characteristics suspected of carrying the mecC gene such as spa types associated with mecC -positive clonal lineages previously described, mecA -negative MRSA isolates, isolates with antimicrobial susceptibility suspected to be mecC -positive or pvl -negative MRSA isolates [ 25 , 26 , 37 , 69 ] ( Table S1 ). In any case, the mecC -MRSA human prevalence detected in most of the studies was very low.…”

Section: Detection Of Mecc -Mrsa Isolates In Humentioning

confidence: 99%

“…In any case, the mecC -MRSA human prevalence detected in most of the studies was very low. Several studies did not identify any mecC -positive S. aureus among included human isolates/samples ( Table 2 ) [ 45 , 46 , 47 , 48 , 49 , 50 , 51 , 52 , 53 , 54 , 55 , 56 , 57 , 58 , 59 , 60 , 61 , 62 , 63 , 64 , 65 , 66 , 67 , 68 , 69 , 70 , 71 , 72 , 73 , 74 ]. In studies in which this gene was detected ( Table 1 ), the prevalence identified, considering the total number of isolates/samples included, was < 1% in most of the cases [ 24 , 27 , 28 , 29 , 32 , 33 , 37 , 40 , 41 , 42 , 43 ], similar to that identified in the first study in which mecC was discovered (approximately 0.04%) [ 16 ].…”

Section: Detection Of Mecc -Mrsa Isolates In Humentioning

confidence: 99%

“…This is in accordance with results obtained in mecC -MRSA isolates from other origins [ 21 ]. In one study performed in Spain, isolates using this criterion were selected in order to identify mecC -MRSA or CA-MRSA isolates [ 69 ]. Although mecC -MRSA was not detected, this resistance phenotype was a valuable marker for Panton-Valentine Leukocidin (PVL)-producer isolates ( Table S1 ).…”

Section: Characterization Of Mecc -Mrsa Human Imentioning

confidence: 99%

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Human mecC-Carrying MRSA: Clinical Implications and Risk Factors

et al. 2020

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A new methicillin resistance gene, named mecC, was first described in 2011 in both humans and animals. Since then, this gene has been detected in different production and free-living animals and as an agent causing infections in some humans. The possible impact that these isolates can have in clinical settings remains unknown. The current available information about mecC-carrying methicillin resistant S. aureus (MRSA) isolates obtained from human samples was analyzed in order to establish its possible clinical implications as well as to determine the infection types associated with this resistance mechanism, the characteristics of these mecC-carrying isolates, their possible relation with animals and the presence of other risk factors. Until now, most human mecC-MRSA infections have been reported in Europe and mecC-MRSA isolates have been identified belonging to a small number of clonal complexes. Although the prevalence of mecC-MRSA human infections is very low and isolates usually contain few resistance (except for beta-lactams) and virulence genes, first isolates harboring important virulence genes or that are resistant to non-beta lactams have already been described. Moreover, severe and even fatal human infection cases have been detected. mecC-carrying MRSA should be taken into consideration in hospital, veterinary and food safety laboratories and in prevention strategies in order to avoid possible emerging health problems.

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Development of a porcine model of skin and soft‐tissue infection caused by Staphylococcus aureus, including methicillin‐resistant strains suitable for testing topical antimicrobial agents

Raška,

Lipový,

Kobzová

et al. 2024

Anim Models and Exp Med

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BackgroundIn view of the ever‐increasing representation of Staphylococcus spp. strains resistant to various antibiotics, the development of in vivo models for evaluation of novel antimicrobials is of utmost importance.MethodsIn this article, we describe the development of a fully immunocompetent porcine model of extensive skin and soft tissue damage suitable for testing topical antimicrobial agents that matches the real clinical situation. The model was developed in three consecutive stages with protocols for each stage amended based on the results of the previous one.ResultsIn the final model, 10 excisions of the skin and underlying soft tissue were created in each pig under general anesthesia, with additional incisions to the fascia performed at the base of the defects and immediately inoculated with Staphylococcus aureus suspension. One pig was not inoculated and used as the negative control. Subsequently, the bandages were changed on Days 4, 8, 11, and 15. At these time points, a filter paper imprint technique (FPIT) was made from each wound for semi‐quantitative microbiological evaluation. Tissue samples from the base of the wound together with the adjacent intact tissue of three randomly selected defects of each pig were taken for microbiological, histopathological, and molecular‐biological examination. The infection with the inoculated S. aureus strains was sufficient during the whole experiment as confirmed by both FPIT and from tissue samples. The dynamics of the inflammatory markers and clinical signs of infection are also described.ConclusionsA successfully developed porcine model is suitable for in vivo testing of novel short‐acting topical antimicrobial agents.

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Multicenter study of clinical non-β-lactam-antibiotic susceptible MRSA strains: Genetic lineages and Panton-Valentine leukocidin (PVL) production

Cited by 8 publications

References 31 publications

Diversity ofStaphylococcus aureusclones in wild mammals in Aragon, Spain, with detection of MRSA ST130‐mecCin wild rabbits

Diversity ofStaphylococcus aureusclones in wild mammals in Aragon, Spain, with detection of MRSA ST130‐mecCin wild rabbits

Human mecC-Carrying MRSA: Clinical Implications and Risk Factors

Development of a porcine model of skin and soft‐tissue infection caused by Staphylococcus aureus, including methicillin‐resistant strains suitable for testing topical antimicrobial agents

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