Multiclass screening method based on solvent extraction and liquid chromatography–tandem mass spectrometry for the determination of antimicrobials and mycotoxins in egg

Capriotti, Anna Laura; Cavaliere, Chiara; Piovesana, Susy; Samperi, Roberto; Laganà, Aldo

doi:10.1016/j.chroma.2012.10.040

Cited by 80 publications

(45 citation statements)

References 28 publications

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“…Mycotoxins are secondary toxic metabolites produced by several filamentous and microscopic fungi, mainly Aspergillus, Penicillium, Fusarium, and Alternaria, growing on various food and feed under a wide range of climatic conditions in the field and during storage (Pfohl-Leszkowicz and Manderville 2007;Desmarchelier et al 2010;Capriotti et al 2012;Paiga et al 2012). The main groups of moulds and mycotoxins of world-wide concern, the most common and of greatest agro-economic importance are the Aspergillus spp.…”

Section: Introductionmentioning

confidence: 98%

High-Throughput Analytical Strategy Based on Modified QuEChERS Extraction and Dispersive Solid-Phase Extraction Clean-up Followed by Liquid Chromatography-Triple-Quadrupole Tandem Mass Spectrometry for Quantification of Multiclass Mycotoxins in Cereals

et al. 2014

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A simple, reliable, efficient, selective and sensitive QuEChERS-based (quick, easy, cheap, effective, rugged and safe) sample preparation strategy, involving an initial partitioning step using acidified acetonitrile (ACN), MgSO 4 , NaCl and citrate buffer salts, combined with dispersive solidphase extraction (d-SPE) clean-up, is proposed for the simultaneous multiclass mycotoxins quantification, including aflatoxins, ochratoxins, fumonisins, trichothecenes and zearalenone, in cereals. The final clear extracts were concentrated under vacuum to near dryness and taken-up with the initial mobile phase (MeOH:H 2 O;70:30, v/v) previous to reversed-phase liquid chromatography-electrospray ionization tandem mass spectrometry (LC-ESI-MS/MS) analysis. Careful optimization of the LC-ESI-MS/MS parameters was achieved in order to attain a fast separation and increased sensitivity. The detection was carried out on a triplequadrupole tandem mass spectrometry (MS/MS) by electrospray ionization in positive ion mode (ESI + ) with multiple reaction monitoring (MRM). Tandem MS conditions were optimised in order to increase selectivity, selecting the best transitions (parent ion to quantifier and qualifier ions) for quantification and identification. The performance of the method was assessed and compared to European Commission (EC) Regulations, by studying the selectivity, specificity, limits of detection (LOD) and quantification (LOQ), linear dynamic range (LDR), matrix effect, accuracy, precision, and uncertainty. Good linearity (r 2 >0.9713) was achieved for all mycotoxins investigated, and LODs (S/N=3) and LOQs (S/ N=10) were below the tolerance levels of mycotoxins set by EC. Recoveries of the extraction process, obtained with different spiked concentrations, ranged from 72.9 to 120.6 %, with relative standard deviations (RSD) lower than 23.0 %. Only in 6 % of all combinations did the RSD values exceed 15 %. Matrix effects were observed by comparing the slope of matrix-matched standard calibration with that of the solvent. The developed method was applied to evaluate the cooccurrence of multiclass mycotoxins in cereals collected at the importation points and consumer habitations at Madeira Island. Samples collected at importation points (15 wheat samples, 4 maize samples and 2 rice samples) showed the presence of DON in three wheat samples, and FB1 and HT-2 in one wheat sample. Three maize samples were detected with FB1 (two samples) and AFG 2 (one sample) whereas one rice sample was detected with ZEN. The results revealed the absence of target mycotoxins on the rice samples collected at consumer habitations. None of the studied cereal samples exceeded the maximum permissible limits or indicative levels set by the EC which means that the particular Madeira Island Electronic supplementary material The online version of this article (

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Section: Introductionmentioning

confidence: 98%

High-Throughput Analytical Strategy Based on Modified QuEChERS Extraction and Dispersive Solid-Phase Extraction Clean-up Followed by Liquid Chromatography-Triple-Quadrupole Tandem Mass Spectrometry for Quantification of Multiclass Mycotoxins in Cereals

et al. 2014

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“…MS) which is in agreement with reference data of the analyte and meets acceptable criteria for the purpose of the analysis. [27][28][29]. As regulators and laboratories strive to increase the scope of the methods to improve consumer safety, this multiclass-multi-analyte approach is expected to become more widely used in routine monitoring.…”

Section: Introductionmentioning

confidence: 99%

Identification in residue analysis based on liquid chromatography with tandem mass spectrometry: Experimental evidence to update performance criteria

Mol

Zomer

López

et al. 2015

Analytica Chimica Acta

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“…Although this procedure required less organic solvent, it has not been used regularly. Most papers focused on the detection in cereal (based) samples, but few papers reported method development for biological samples like hen eggs and pig plasma [24,28,29]. Sample preparation time and detection levels significantly improved from 1-50 mg.kg -1 to trace analysis at low μg.kg -1 levels by switching from HPLC with UV or DAD detection to UPLC with (tandem) MS detection [26,30].…”

Section: Introductionmentioning

confidence: 99%

Development and validation of ultra-high-performance liquid chromatography–tandem mass spectrometry methods for the simultaneous determination of beauvericin, enniatins (A, A1, B, B1) and cereulide in maize, wheat, pasta and rice

Decleer

Rajković

Sas

et al. 2016

Journal of Chromatography A

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Multiclass screening method based on solvent extraction and liquid chromatography–tandem mass spectrometry for the determination of antimicrobials and mycotoxins in egg

Cited by 80 publications

References 28 publications

High-Throughput Analytical Strategy Based on Modified QuEChERS Extraction and Dispersive Solid-Phase Extraction Clean-up Followed by Liquid Chromatography-Triple-Quadrupole Tandem Mass Spectrometry for Quantification of Multiclass Mycotoxins in Cereals

High-Throughput Analytical Strategy Based on Modified QuEChERS Extraction and Dispersive Solid-Phase Extraction Clean-up Followed by Liquid Chromatography-Triple-Quadrupole Tandem Mass Spectrometry for Quantification of Multiclass Mycotoxins in Cereals

Identification in residue analysis based on liquid chromatography with tandem mass spectrometry: Experimental evidence to update performance criteria

Development and validation of ultra-high-performance liquid chromatography–tandem mass spectrometry methods for the simultaneous determination of beauvericin, enniatins (A, A1, B, B1) and cereulide in maize, wheat, pasta and rice

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