Multicolor flow cytometry for evaluation of platelet surface antigens and activation markers

Velzen, Jeroen F. van; Gorkom, Britta A P Laros-van; Pop, Gheorghe; Heerde, Waander L. van

doi:10.1016/j.thromres.2012.02.041

Cited by 87 publications

(53 citation statements)

References 34 publications

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“…Different probes or markers can simultaneously be analysed by using multicolor flow cytometry. [17] The function of "gating" or isolating particular cell clusters in flow cytometry enables classification and investigation of platelets in the mixed populations of cells like found in whole blood. [3] Two parameters are employed to facilitate platelet gating namely 1) forward light scatter (FSC) which arranges cells according to size, and 2) the use of a platelet-specific antibody like CD41 or CD42b.…”

Section: Gating Strategiesmentioning

confidence: 99%

“…This technique also decreases variation since fewer sample preparation is required and is an effortless method to study platelet population. [17] The reliability of the population of platelets in a whole blood sample is determined by the gating strategy employed. [11,19,20] Van Velzen and colleagues have recently investigated the effect of different platelet gating strategies.…”

Section: Gating Strategiesmentioning

confidence: 99%

“…By using a platelet specific marker not only eliminate coincidence but also excludes complexes platelets form with monocytes and decreases possible contamination. [17] This research of van Velzen et al was done for a fixated whole blood sample. In this investigation similar results were found for an unfixed whole blood sample.…”

Section: Gating Strategiesmentioning

confidence: 99%

“…They incorporated all 4 the mentioned antibodies; however, the conjugated fluorochromes differed from the probes used in this study and the samples were fixated before analysis. [17] …”

Section: Blood Preparation For Flow Cytometrymentioning

confidence: 99%

“…[2] Van Velzen et al also found the expression of CD62P and CD63 to be increased when platelets were exposed to thrombin activation in a fixated sample. [17] Period between sampling and analysis CD41 is a calcium-dependent compound of GPIIb/IIIa. The interactions between cells and the cells with the matrix are mediated by GPIIb/IIIa.…”

Section: Activation Of Plateletsmentioning

confidence: 99%

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Flow cytometric comparison of platelets from a whole blood and finger-prick sample: impact of 24 hours storage

2013

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In this study, we investigate the validity and laboratory utility of flow cytometry when analyzing platelet activation by studying CD41, CD42b, CD62P and CD63. We compare flow cytometry results from citrated whole blood and finger-prick samples directly after collection and also after storing both a finger-prick and whole blood sample for 24 hours. Citrated whole blood and finger-prick samples were taken from three healthy individuals on two occasions, and a total of 60 000 cells were analyzed for each of the 4 phycoerythrin-labelled monoclonal antibodies. Half of each sample was analyzed immediately after sampling while the other half was kept in the fridge at 6°C for 24 hours before analysis. No significant difference was found between the sampling methods or the period of time before analysis. Results therefore suggest that an appropriately prepared finger-prick sample can be used for platelet function analysis, and samples can be stored for 24 hours in the fridge at 6°C before analysis.

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Section: Gating Strategiesmentioning

confidence: 99%

Section: Gating Strategiesmentioning

confidence: 99%

Section: Gating Strategiesmentioning

confidence: 99%

“…They incorporated all 4 the mentioned antibodies; however, the conjugated fluorochromes differed from the probes used in this study and the samples were fixated before analysis. [17] …”

Section: Blood Preparation For Flow Cytometrymentioning

confidence: 99%

Section: Activation Of Plateletsmentioning

confidence: 99%

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Flow cytometric comparison of platelets from a whole blood and finger-prick sample: impact of 24 hours storage

2013

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Antibacterial Sericin Cryogels Promote Hemostasis by Facilitating the Activation of Coagulation Pathway and Platelets

Zhu

Liu

Qin

et al. 2022

Adv Healthcare Materials

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Cryogels, with high water/blood absorption, have great potential for rapid hemostasis. In this study, a hemostatic and antibacterial sericin‐methacryloyl/Ag cryogel (SMC@Ag) based on freeze polymerization of methacryloyl‐modified sericin and in situ reduction of silver ions is developed. The combination of interconnected micropores and Ag NPs endows the cryogel with high water/blood absorption, and outstanding hemostatic and antibacterial performance. SMC@Ag shows much better hemostatic performance than the commercial gelatin sponge in rat liver injury, tail amputation, and femoral artery injury models. Furthermore, the excellent hemostatic activity of SMC@Ag is due to facilitating the coagulation pathway activation and enhancing the platelets adhesion during coagulation process. Overall, SMC@Ag cryogel with excellent hemostatic and antibacterial performance is a suitable candidate for traumatic hemorrhage and wound healing.

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Platelet activation and arterial peripheral serotonin turnover in cardiac remodeling associated to aortic stenosis

et al. 2014

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Peripheral serotonin (5-HT) has been involved in adverse cardiac remodeling and valve fibrosis. The peripheral levels of 5-HT mainly depend on its release from activated platelets and degradation by monoamine oxidase A (MAO-A). The SERAOPI study investigated the relationship between arterial serotoninergic system, degree of platelet activation and cardiac remodeling, in patients with aortic valve stenosis (AS). Thirty patients with severe AS and 15 control subjects underwent transthoracic echocardiography, radial, and aortic arterial blood sampling. Measurements of 5-HT and its MAO-Adependent degradation product, 5-HIAA, were performed by HPLC. Arterial platelet activation was assessed by flow cytometry analysis of platelet surface expression of P-selectin and activated integrin GPIIb/IIIa. Activated platelets and arterial plasma 5-HT increased in AS patients as compared to control subjects (P-selectin 1.08 6 0.2MFI vs. 0.49 6 0.1MFI, P 5 0.04; GPIIb/IIIa 0.71 6 0.1MFI vs. 0.35 6 0.1MFI; P 5 0.0015 and arterial plasma 5-HT 11.55 6 1.6 nM vs. 6.18 6 0.7 nM, P 5 0.028, respectively). Moreover, 5-HT was strongly correlated to left ventricular hypertrophy assessed by echocardiography. The correlation was independent of cardiovascular risk comorbidities and others echocardiographic AS parameters. Finally, plasma 5-HIAA increased in AS patients (74.64 6 9.7 nM vs. 37.16 6 4.1 nM; P 5 0.0002) indicating a higher 5-HT degradation rate by MAO-A. Platelet activation, arterial circulating serotonin, and serotonin degradation increased in patients with AS. These observations suggest that the serotoninergic system may contribute to the pathogenesis of AS including valve fibrosis and adverse ventricular remodeling.

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Multicolor flow cytometry for evaluation of platelet surface antigens and activation markers

Cited by 87 publications

References 34 publications

Flow cytometric comparison of platelets from a whole blood and finger-prick sample: impact of 24 hours storage

Flow cytometric comparison of platelets from a whole blood and finger-prick sample: impact of 24 hours storage

Antibacterial Sericin Cryogels Promote Hemostasis by Facilitating the Activation of Coagulation Pathway and Platelets

Platelet activation and arterial peripheral serotonin turnover in cardiac remodeling associated to aortic stenosis

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