Multicolor fluorescence imaging for early detection of the hypersensitive reaction to tobacco mosaic virus

Chaerle, Laury; Lenk, Sándor; Hagenbeek, Dik; Buschmann, Claus; Straeten, Dominique Van Der

doi:10.1016/j.jplph.2006.01.011

Cited by 86 publications

(66 citation statements)

References 32 publications

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“…Earlier, several studies reported already the suppression of photosynthesis in different plant/virus interactions. Lowered efficiency of energy flow was caused by structural/functional disturbances in membrane or directly by diminished chlorophyll content (Alm asi et al, 2001;Chaerle et al, 2007). In our experiments, chlorophyll a imaging results confirmed the substantial decrease of PSII efficiency in ObPV-inoculated leaves detected by PEA analysis.…”

Section: Discussionsupporting

confidence: 83%

“…The infected plants also showed a reduction in the efficiency of excitation capture in PSII by photoprotective thermal dissipation (Rahoutei et al, 2000). In recent years non-invasive chlorophyll fluorescence imaging techniques have been developed to continuously follow-up the physiological status and performance of plants, as well as to detect stress-induced deviations presymptomatically (Chaerle et al, 2007;Rolfe and Scholes, 2010). Modulation of photosynthetic activity can be revealed by measurements of chlorophyll a fluorescence emission.…”

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confidence: 99%

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Comparison of a compatible and an incompatible pepper-tobamovirus interaction by biochemical and non-invasive techniques: Chlorophyll a fluorescence, isothermal calorimetry and FT-Raman spectroscopy

Rys

Juhász

Surówka

et al. 2014

Plant Physiology and Biochemistry

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imaging technique. PMMoV inoculation also led to a significant rise of ion leakage and heat emission, to the up-regulation of the pepper CCD gene as well as to decreased PSII efficiency, but these responses were much weaker than in the case of ObPV inoculation. Chlorophyll b and total carotenoid contents as measured by spectrophotometric methods were not significantly influenced by any virus inoculations when these pigment contents were calculated on leaf surface basis. On the other hand, near-infrared FTRaman spectroscopy showed an increase of carotenoid content in ObPV-inoculated leaves suggesting that the two techniques detect different sets of compounds.

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Section: Discussionsupporting

confidence: 83%

mentioning

confidence: 99%

Comparison of a compatible and an incompatible pepper-tobamovirus interaction by biochemical and non-invasive techniques: Chlorophyll a fluorescence, isothermal calorimetry and FT-Raman spectroscopy

Rys

Juhász

Surówka

et al. 2014

Plant Physiology and Biochemistry

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show abstract

“…8C). UV illumination also indicated that the induction of HR was associated with the accumulation of blue autofluorescing compounds, as is commonly seen upon virus-induced HR in Nicotiana species (Costet et al, 2002;Chaerle et al, 2007). Consistent with the enhanced HR in THF1-silenced tissues, blue autofluorescing compounds were much more abundant in pTV:THF1 plants following activation of N9 (Fig.…”

Section: Thf1 Suppresses N9-mediated Hr Following Recognition Of the supporting

confidence: 67%

The Chloroplastic Protein THF1 Interacts with the Coiled-Coil Domain of the Disease Resistance Protein N′ and Regulates Light-Dependent Cell Death

et al. 2016

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One branch of plant immunity is mediated through nucleotide-binding/Leu-rich repeat (NB-LRR) family proteins that recognize specific effectors encoded by pathogens. Members of the I2-like family constitute a well-conserved subgroup of NB-LRRs from Solanaceae possessing a coiled-coil (CC) domain at their N termini. We show here that the CC domains of several I2-like proteins are able to induce a hypersensitive response (HR), a form of programmed cell death associated with disease resistance. Using yeast two-hybrid screens, we identified the chloroplastic protein Thylakoid Formation1 (THF1) as an interacting partner for several I2-like CC domains. Co-immunoprecipitations and bimolecular fluorescence complementation assays confirmed that THF1 and I2-like CC domains interact in planta and that these interactions take place in the cytosol. Several HR-inducing I2-like CC domains have a negative effect on the accumulation of THF1, suggesting that the latter is destabilized by active CC domains. To confirm this model, we investigated N9, which recognizes the coat protein of most Tobamoviruses, as a prototypical member of the I2-like family. Transient expression and gene silencing data indicated that THF1 functions as a negative regulator of cell death and that activation of full-length N9 results in the destabilization of THF1. Consistent with the known function of THF1 in maintaining chloroplast homeostasis, we show that the HR induced by N9 is light-dependent. Together, our results define, to our knowledge, novel molecular mechanisms linking light and chloroplasts to the induction of cell death by a subgroup of NB-LRR proteins.

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“…Phenolic compounds, secondary metabolism molecular components, including precursors for antifungal compounds, cytoderm reinforcement, and SA, help to control plant diseases. Under UV excitation, tobacco cells can present fluorescence attributable to the accumulation and oxidation of phenolic compounds, such as scopoletin, scopolin, and ferulic acid (50,51). After 108 h of incubation with PeBL1, phenolic compounds accumulated in the tobacco cells and were observed in blue under fluorescence microscopy (Fig.…”

Section: Expression and Purification Of Recombinant Proteinmentioning

confidence: 98%

PeBL1, a Novel Protein Elicitor from Brevibacillus laterosporus Strain A60, Activates Defense Responses and Systemic Resistance in Nicotiana benthamiana

Wang

Yang

Guo

et al. 2015

Appl Environ Microbiol

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We report the identification, characterization, and gene cloning of a novel protein elicitor (PeBL1) secreted from Brevibacillus laterosporus strain A60. Through a purification process consisting of ion-exchange chromatography and high-performance liquid chromatography (HPLC), we isolated a protein that was identified by electrospray ionization quadrupole time of flight tandem mass spectrometry ( , and PAL were upregulated to varying degrees by PeBL1. This research not only provides insights into the mechanism by which beneficial bacteria activate plant systemic resistance, but also sheds new light on a novel strategy for biocontrol using strain A60.

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Multicolor fluorescence imaging for early detection of the hypersensitive reaction to tobacco mosaic virus

Cited by 86 publications

References 32 publications

Comparison of a compatible and an incompatible pepper-tobamovirus interaction by biochemical and non-invasive techniques: Chlorophyll a fluorescence, isothermal calorimetry and FT-Raman spectroscopy

Comparison of a compatible and an incompatible pepper-tobamovirus interaction by biochemical and non-invasive techniques: Chlorophyll a fluorescence, isothermal calorimetry and FT-Raman spectroscopy

The Chloroplastic Protein THF1 Interacts with the Coiled-Coil Domain of the Disease Resistance Protein N′ and Regulates Light-Dependent Cell Death

PeBL1, a Novel Protein Elicitor from Brevibacillus laterosporus Strain A60, Activates Defense Responses and Systemic Resistance in Nicotiana benthamiana

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