Multicolor Tunable Polymeric Nanoparticle from the Tetraphenylethylene Cage for Temperature Sensing in Living Cells

Abstract

It is meaningful but challenging to develop a fluorescent probe for temperature sensing in living cells because it should possess the features of good cytocompatibility, easy read out, and high resolution. Herein, we successfully synthesized emissive star-like cage-based organic temperature-sensitive polymers that can assemble into nanoparticles in aqueous solution. The obtained nanoparticle can be easily tuned to full-color emission (including white light emission) with a temperature resolution of at least 0.5 °C by encapsulating different doses of guest dyes ((4-dimethylamino-2′-butoxychalcone (DMBC) and Nile Red (NR)) through a cascade Förster resonance energy transfer (FRET) effect. Moreover, the white light emission polymeric hybrid nanoparticles exhibit reversible stimuli response toward temperature and can be used as probes for temperature sensing in live cells through their fluorescent color variation between white and orange emission with good cytocompatibility.

Emerging microfluidic chips on medical application

“…于⽣物标志物的检测诊断，实际应⽤如图 4(a)所示，Mohamed 等⼈ [40] 开发并验证了⼀种超灵敏的单细胞蛋 焦和胶体颗粒合成成为现实 [45] 。近年来出现了许多基于温度信号的⽣化检测的报道，如 Zhen Wang 等⼈ [46] 设计并合成了⼀种具有温敏性的杂化纳⽶粒⼦， 这种⽣物相容的热敏性材料可作为活细胞的温度探针。 然⽽，…”

In Situ Control of Multicolor Luminescence over the Entire Visible Spectral Region in a Single Chromophore by Sol–Gel Transformation and Dynamic Metal–Ligand Coordination

“…[1][2][3][4][5] In recent years, these smart materials have drawn significant interest due to their wide range of applications in sophisticated technologies and daily life. [6][7][8] In particular, research on multicolor emitting chromophores is of fundamental importance, [9][10][11] as they can serve as key precursors in achieving advanced functions of signal generation, [12][13][14][15] information storage and processing, [16][17][18] displays, [19][20][21][22] optical imaging, [23][24][25] and anticounterfeiting. [26][27][28][29][30][31] Control of molecular switches has been demonstrated as an effective way to modulate the luminescence conversion processes of chromophores.…”

A Dual‐Excitation Decoding Strategy Based on NIR Hybrid Nanocomposites for High‐Accuracy Thermal Sensing

“…In recent years, in vivo thermal sensing has shown great promise for physiological studies, medical diagnosis, and especially hyperthermia treatment. [ 1 ] The contactless optical thermometry with both absorption and emission bands lying within the near infrared (NIR) biological window (750–1700 nm) enables deep‐tissue and noninvasive real‐time monitoring of temperature. [ 2 ] Among them, ratiometric (or self‐referenced) thermometry is well‐established, wherein the intensity ratio of two nonoverlapping emissions with distinct thermal responses is conventionally defined as the thermosensitive parameter.…”