Multicolor Visual Detection of Deoxynivalenol in Grain Based on Magnetic Immunoassay and Enzymatic Etching of Plasmonic Gold Nanobipyramids

Abstract

In this study, a multicolor visual method based on a magnetic immunoassay and enzyme-induced gold nanobipyramids (Au NBPs) etching was developed for deoxynivalenol (DON) detection. The magnetic beads modified with high affinity DON monoclonal antibodies were used as a carrier for target enrichment and signal transformation and the Au NBPs with excellent plasmonic optical properties were served as enzymatic etching substrates. The oxidation state TMB, which was generated through catalysis of horseradish peroxidase (HRP), induced the etching of plasmonic Au NBPs, resulting in the longitudinal peak blue-shift of local surface plasmon resonance (LSPR). Correspondingly, Au NBPs with various aspect ratios displayed a variety of individual colors which were visualized by the naked eye. The LSPR peak shift was linearly related to the DON concentration in the range of 0~2000 ng/mL and the detection limit was 57.93 ng/mL. The recovery for naturally contaminated wheat and maize at different concentrations ranged from 93.7% to 105.7% with a good relative standard deviation below 11.8%. Through observing the color change in Au NBPs, samples with overproof DON could be screened preliminarily by the naked eye. The proposed method has the potential to be applied in on-site rapid screening of mycotoxins in grain. In addition, the current multicolor visual method only used for the simultaneous detection of multiple mycotoxins is in urgent need of a breakthrough to overcome the limitation of single mycotoxin detection.

Zeolitic Imidazolate Framework-8 Composite-Based Enzyme-Linked Aptamer Assay for the Sensitive Detection of Deoxynivalenol

“…Table S1: Sequence information of the oligonucleotides used in this work; Table S2: Comparison of different methods for DON detection; Table S3: Detection of DON in actual samples by the proposed ELAA method; and Table S4: Comparison of the detection limit and cost between ELISA and the proposed ELAA method. References [ 39 , 40 , 41 , 42 , 43 , 44 , 45 ] are cited in Supplementary Materials.…”