Multicompartmental traumatic injury induces sex-specific alterations in the gut microbiome

Munley, Jennifer A.; Kelly, Lauren; Park, Gwoncheol; Gillies, Gwendolyn S; Pons, Erick E; Kannan, Kolenkode B.; Whitley, Elizabeth M.; Bible, Letitia; Efron, Philip A.; Nagpal, Ravinder; Mohr, Alicia M.

doi:10.1097/ta.0000000000003939

Cited by 12 publications

(24 citation statements)

References 47 publications

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“…Gut bacterial and fungal microbiome profiles were measured and analyzed as per our previously described methods. 96 The fecal samples were preserved at −80°C until microbial DNA extraction. The QIAmp PowerFecal Pro DNA Kit (Qiagen) was used to extract genomic DNA from a 200 mg fecal specimen, following the manufacturer’s guidelines.…”

Section: Methodsmentioning

confidence: 99%

A modified Mediterranean-style diet enhances brain function via specific gut-microbiome-brain mechanisms

Park,

Kadyan,

Hochuli

et al. 2024

Gut Microbes

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Alzheimer’s disease (AD) is a debilitating brain disorder with rapidly mounting prevalence worldwide, yet no proven AD cure has been discovered. Using a multi-omics approach in a transgenic AD mouse model, the current study demonstrated the efficacy of a modified Mediterranean-ketogenic diet (MkD) on AD-related neurocognitive pathophysiology and underlying mechanisms related to the gut-microbiome-brain axis. The findings revealed that MkD induces profound shifts in the gut microbiome community and microbial metabolites. Most notably, MkD promoted growth of the Lactobacillus population, resulting in increased bacteria-derived lactate production. We discovered elevated levels of microbiome- and diet-derived metabolites in the serum as well, signaling their influence on the brain. Importantly, these changes in serum metabolites upregulated specific receptors that have neuroprotective effects and induced alternations in neuroinflammatory-associated pathway profiles in hippocampus. Additionally, these metabolites displayed strong favorable co-regulation relationship with gut-brain integrity and inflammatory markers, as well as neurobehavioral outcomes. The findings underscore the ameliorative effects of MkD on AD-related neurological function and the underlying gut-brain communication via modulation of the gut microbiome-metabolome arrays.

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Section: Methodsmentioning

confidence: 99%

A modified Mediterranean-style diet enhances brain function via specific gut-microbiome-brain mechanisms

Park,

Kadyan,

Hochuli

et al. 2024

Gut Microbes

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“…This study also demonstrated shifts in beta-diversity in both PT and PT/CS at days three and seven, with unique microbial composition differences between these groups at each time point [ 82 ]. A shorter-term study of the same polytrauma model in both males and females demonstrated that at baseline pre-injury, females had a higher Shannon index (alpha-diversity) than males and after injury, females and males had similar alpha-diversity [ 83 ]. This study also showed that males and females subjected to polytrauma demonstrated differences in beta-diversity 2 days postinjury, in addition to unique microbiome compositions [ 83 ].…”

Section: Traumamentioning

confidence: 99%

“…A shorter-term study of the same polytrauma model in both males and females demonstrated that at baseline pre-injury, females had a higher Shannon index (alpha-diversity) than males and after injury, females and males had similar alpha-diversity [ 83 ]. This study also showed that males and females subjected to polytrauma demonstrated differences in beta-diversity 2 days postinjury, in addition to unique microbiome compositions [ 83 ]. The evidence in these studies shows the impact of polytrauma on the intestinal microbiome and also underscores the need for more studies investigating the role of sex in the development of the pathobiome and the need to uncover the underlying mechanisms behind postinjury dysbiosis.…”

Section: Traumamentioning

confidence: 99%

“…This study also highlighted an increased descending colonic injury in both groups of multiple injuries with or without chronic stress characterized by lamina propria edema and the infiltration of inflammatory cells seven days postinjury [ 82 ]. Finally, a study of the same model of polytrauma in both male and female rats showed increased plasma occludin and lipopolysaccharide-binding proteins in both PT and PT/CS groups of males and females, suggestive of increased intestinal permeability, only two days after injury [ 83 ]. This same study also showed that males subjected to PT had higher levels of plasma occludin and males subjected to PT/CS had higher plasma LBP than their female counterparts 48 h postinjury [ 83 ].…”

Section: Traumamentioning

confidence: 99%

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The Intestinal Microbiome after Traumatic Injury

et al. 2023

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The intestinal microbiome plays a critical role in host immune function and homeostasis. Patients suffering from—as well as models representing—multiple traumatic injuries, isolated organ system trauma, and various severities of traumatic injury have been studied as an area of interest in the dysregulation of immune function and systemic inflammation which occur after trauma. These studies also demonstrate changes in gut microbiome diversity and even microbial composition, with a transition to a pathobiome state. In addition, sex has been identified as a biological variable influencing alterations in the microbiome after trauma. Therapeutics such as fecal transplantation have been utilized to ameliorate not only these microbiome changes but may also play a role in recovery postinjury. This review summarizes the alterations in the gut microbiome that occur postinjury, either in isolated injury or multiple injuries, along with proposed mechanisms for these changes and future directions for the field.

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“…Trauma, of whatever cause, either a clean surgical wound or a severe traumatic injury, leads to an alteration in the beta diversity of resident microbiota due to the oxidative stress caused by both tissue injury and anesthesia. The alteration of the microbiota involves both the intestinal microbiota-being the most studied and definitely considered as a generalized reaction-and the local microbiota, in this case, that of the skin [1][2][3][4].…”

Section: Introductionmentioning

confidence: 99%

Looking for the Ideal Probiotic Healing Regime

et al. 2023

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Wound healing is a multi-factorial response to tissue injury, aiming to restore tissue continuity. Numerous recent experimental and clinical studies clearly indicate that probiotics are applied topically to promote the wound-healing process. However, the precise mechanism by which they contribute to healing is not yet clear. Each strain appears to exert a distinctive, even multi-factorial action on different phases of the healing process. Given that a multi-probiotic formula exerts better results than a single strain, the pharmaceutical industry has embarked on a race for the production of a formulation containing a combination of probiotics capable of playing a role in all the phases of the healing process. Hence, the object of this review is to describe what is known to date of the distinctive mechanisms of each of the most studied probiotic strains in order to further facilitate research toward the development of combinations of strains and doses, covering the whole spectrum of healing. Eleven probiotic species have been analyzed, the only criterion of inclusion being a minimum of two published research articles.

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Multicompartmental traumatic injury induces sex-specific alterations in the gut microbiome

Abstract: Severe traumatic injury in a rodent model alters the intestinal microbiome in a sex-specific manner. Females have increased diversity and unique microbial signatures compared to males. These sex-specific gut dysbiosis arrays may influence outcomes after multicompartmental trauma.

Cited by 12 publications

References 47 publications

A modified Mediterranean-style diet enhances brain function via specific gut-microbiome-brain mechanisms

A modified Mediterranean-style diet enhances brain function via specific gut-microbiome-brain mechanisms

The Intestinal Microbiome after Traumatic Injury

Looking for the Ideal Probiotic Healing Regime

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