Multicore Magnetic Nanoparticles (MMNPs) Doped with Cs and FITC for the Determination of Biomarker in Serum using ICP-MS

Abstract

Multicore magnetic nanoparticles (MMNPs) doped with Cs and FITC (Cs/FITC-doped MMNPs) were synthesized for the extraction and determination of biomarkers using inductively coupled plasma-mass spectrometry (ICP-MS). For demonstration, the MMNPs were used for magnetic separation to extract CA19-9 in serum nonspecifically, and the doped Cs was used as an internal standard for the ratiometric measurement of the tagged particle. This ratiometric method compensated for the particle loss in a magnetic separation and suppressed the signal fluctuation which increased the calibration linearity significantly. The obtained detection limit was 0.02 units/mL of CA19-9, which is more than 300 times lower than that reported by the ICP-MS with element tagging and about 500-fold improved compared to ELISA.

Atomic spectrometry update: review of advances in atomic spectrometry and related techniques

“…The method was used to determine glycoproteins in real human serum with recoveries of over 97%. 35,36 1.1.2 Nebulisation. By taking advantage of the amplifying effect of the Au-NP the LOD was 86 cells, which is comparable with electrochemical detection but faster.…”

Lanthanide-Doped Upconversion-Linked Immunosorbent Assay for the Sensitive Detection of Carbohydrate Antigen 19-9

“…The UCNPs-SiO -NH 2 were conjugated to a secondary antibody via standard EDC/sulfo-chemistry (Ko and Lim, 2014). In a typical synthesis, the presynthesized UCNPs@SiO 2 -NH 2 were dispersed in 10 ml of PBS solution at room temperature under slight magnetic stirring for 2 h. Then, 1-ethyl-3-(dimethylaminopropyl) carbodiimide (EDC) and N-hydroxysuccinimide (NHS) mixture (1:1) were added and mixed for 1 h. The secondary antibody (Ab 2 , Abcam) was introduced into the system and kept going for another 2 h under regular shaking.…”

Zero-Field and Field-Induced Interactions between Multicore Magnetic Nanoparticles

“…Recently, MCMNPs have attracted a lot of research attention due to their exceptionally wide range of potential biomedical and biotechnological applications [3]. They are considered to be prominent in such areas as magnetic bioseparation and immunoassays [4,5,6], targeted drug delivery [7,8], magnetic resonance imaging [9,10,11], magnetic particle imaging [12,13,14], and magnetic hyperthermia [15,16,17,18].…”