Multidimensional controllable fabrication of tumor spheroids based on a microfluidic device

Abstract

Multicellular tumor spheroids (MCTSs) are in vitro solid tumor models with physiologic relevance. To achieve robust process control, an MCTS fabrication method that combines cell membrane engineering and droplet microfluidic...

Advanced sequencing-based high-throughput and long-read single-cell transcriptome analysis

“…Currently, microfluidic-based platforms dominate the field of high-throughput scRNA-Seq due to their remarkable advantages in terms of miniaturization, integration, and parallel operations. [24][25][26] However, cell utilization was still limited by the Poisson distribution restriction. To improve cell utilization, various strategies have been developed.…”

Integrating microfluidics and synthetic biology: advancements and diverse applications across organisms

“…116 An organoid is a miniature structure composed of ex vivo cells that mimics the structure and function of a real organ. 117 Growing in a three-dimensional configuration, the cells can form a complex system to act as an organ like structure simulating the human body. 116 Culturing patient primary cells on this platform creates a more accurate representation of each patients unique phenotypic behavior to various drug treatments and genetic modifications.…”

Microfluidic-based platforms for cell-to-cell communication studies

“…Recently, there is a growing interest in exploring cell membrane engineering methods for co-culturing heterogeneous cells. Cell membrane engineering involves the use of chemical or genetic methods to introduce specific target molecules into cell membranes, thereby actively regulating and supporting cellular function [113]. Compared to the SB method, this approach effectively decreases the probability of toxicity and immunogenicity caused by introducing foreign scaffold materials, which is feasible to achieve controllable cell aggregation and encapsulation within a hydrogel shell on a microfluidic device.…”