Multidisciplinary approaches for enzyme biocatalysis in pharmaceuticals: protein engineering, computational biology, and nanoarchitectonics

Kim, Suhyeon; Ga, Seongmin; Bae, Hayeon; Sluyter, Ronald; Konstantinov, Konstantin; Shrestha, Lok Kumar; Kim, Yong Ho; Kim, Jung Ho; Ariga, Katsuhiko

doi:10.1039/d3ey00239j

Cited by 7 publications

(1 citation statement)

References 226 publications

(347 reference statements)

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“…They used lipozyme TL IM ® absorbed on silica particles and obtained high yields for the synthesis of 6-O-palmitoylglucose (95%) in only 30 min [148]. This field has a strong potential for improvement in the coming years, especially if we combine all the possible advances, such as combining several enzymes or considering nanoarchitectonics, which enables the use of innovative strategies for integrating enzymes into continuous flow systems [149].…”

Section: Interest Of Flow Chemistrymentioning

confidence: 99%

Latest Trends in Lipase-Catalyzed Synthesis of Ester Carbohydrate Surfactants: From Key Parameters to Opportunities and Future Development

Spalletta,

Joly,

Martin

2024

IJMS

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Carbohydrate-based surfactants are amphiphilic compounds containing hydrophilic moieties linked to hydrophobic aglycones. More specifically, carbohydrate esters are biosourced and biocompatible surfactants derived from inexpensive renewable raw materials (sugars and fatty acids). Their unique properties allow them to be used in various areas, such as the cosmetic, food, and medicine industries. These multi-applications have created a worldwide market for biobased surfactants and consequently expectations for their production. Biobased surfactants can be obtained from various processes, such as chemical synthesis or microorganism culture and surfactant purification. In accordance with the need for more sustainable and greener processes, the synthesis of these molecules by enzymatic pathways is an opportunity. This work presents a state-of-the-art lipase action mode, with a focus on the active sites of these proteins, and then on four essential parameters for optimizing the reaction: type of lipase, reaction medium, temperature, and ratio of substrates. Finally, this review discusses the latest trends and recent developments, showing the unlimited potential for optimization of such enzymatic syntheses.

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Section: Interest Of Flow Chemistrymentioning

confidence: 99%

Latest Trends in Lipase-Catalyzed Synthesis of Ester Carbohydrate Surfactants: From Key Parameters to Opportunities and Future Development

Spalletta,

Joly,

Martin

2024

IJMS

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Immobilized enzymes and their applications in environmental sustainability of food production and biodegradation

Morya,

Skiba,

Dinesh

et al. 2024

Enzyme Biotechnology for Environmental Sustainability

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Engineering Bacterial Chitinases for Industrial Application: From Protein Engineering to Bacterial Strains Mutation! A Comprehensive Review of Physical, Molecular, and Computational Approaches

Han,

Ullah,

Andoh

et al. 2024

J. Agric. Food Chem.

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Bacterial chitinases are integral in breaking down chitin, the natural polymer in crustacean and insect exoskeletons. Their increasing utilization across various sectors such as agriculture, waste management, biotechnology, food processing, and pharmaceutical industries highlights their significance as biocatalysts. The current review investigates various scientific strategies to maximize the efficiency and production of bacterial chitinases for industrial use. Our goal is to optimize the heterologous production process using physical, molecular, and computational tools. Physical methods focus on isolating, purifying, and characterizing chitinases from various sources to ensure optimal conditions for maximum enzyme activity. Molecular techniques involve gene cloning, site-directed mutation, and CRISPR-Cas9 gene editing as an approach for creating chitinases with improved catalytic activity, substrate specificity, and stability. Computational approaches use molecular modeling, docking, and simulation techniques to accurately predict enzyme–substrate interactions and enhance chitinase variants’ design. Integrating multidisciplinary strategies enables the development of highly efficient chitinases tailored for specific industrial applications. This review summarizes current knowledge and advances in chitinase engineering to serve as an indispensable guideline for researchers and industrialists seeking to optimize chitinase production for various uses.

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Multidisciplinary approaches for enzyme biocatalysis in pharmaceuticals: protein engineering, computational biology, and nanoarchitectonics

Abstract: Enzyme biocatalysis is reshaping pharmaceutical synthesis, offering sustainable and efficient pathways for drug discovery and production. This paradigm shift towards eco-friendly methodologies addresses concerns inherent in traditional chemical synthesis. Enzymes,...

Cited by 7 publications

References 226 publications

Latest Trends in Lipase-Catalyzed Synthesis of Ester Carbohydrate Surfactants: From Key Parameters to Opportunities and Future Development

Latest Trends in Lipase-Catalyzed Synthesis of Ester Carbohydrate Surfactants: From Key Parameters to Opportunities and Future Development

Immobilized enzymes and their applications in environmental sustainability of food production and biodegradation

Engineering Bacterial Chitinases for Industrial Application: From Protein Engineering to Bacterial Strains Mutation! A Comprehensive Review of Physical, Molecular, and Computational Approaches

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