Multidrug resistance-associated ABC transporters – too much of one thing, good for nothing

Procházková, Jiřina; Lánová, Martina Kohutková; Pachernı́k, Jiřı́

doi:10.1515/bmc-2012-0006

Cited by 4 publications

(1 citation statement)

References 101 publications

(105 reference statements)

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“…SDR transporters are specifically used to transport one or a group of closely related drugs. Almost all SDR transporters have the same structural unit of {TMD} 2 -{NBD} 2 (Ross et al, 1990), but MDR transporters often exist as {TMD-NBD} 2 , such as P-gp (Prochazkova et al, 2012), MsbA (Chang, 2003), andLmrA (van Veen et al, 1998). However, there are exceptions.…”

Section: Discussionmentioning

confidence: 99%

A Putative Efflux Transporter of the ABC Family, YbhFSR, in Escherichia coli Functions in Tetracycline Efflux and Na+(Li+)/H+ Transport

et al. 2020

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ATP-binding cassette transporters are ubiquitous in almost all organisms. The Escherichia coli genome is predicted to encode 69 ABC transporters. Eleven of the ABC transporters are presumed to be exporters, of which seven are possible drug export transporters. There has been minimal research on the function of YbhFSR, which is one of the putative drug resistance exporters. In this study, the ybhF gene of this transporter was characterized. Overexpression and knockout strains of ybhF were constructed. The ATPase activity of YbhF was studied using the malachite green assay, and the efflux abilities of knockout strains were demonstrated by using ethidium bromide (EB) as a substrate. The substrates of YbhFSR efflux, examined with the minimum inhibitory concentration (MIC), were determined to be tetracycline, oxytetracycline, chlortetracycline, doxycycline, EB, and Hoechst33342. Furthermore, tetracycline and EB efflux and accumulation experiments confirmed that the substrates of YbhFSR were tetracyclines and EB. The MIC assay and the fluorescence test results showed that tetracyclines are likely to be the major antibiotic substrate of YbhFSR. The existence of the signature NatA motif suggested that YbhFSR may also function as a Na + /H + transporter. Overexpression of YbhF in E. coli KNabc lacking crucial Na + /H + transporters conferred tolerance to NaCl, LiCl, and an alkaline pH. Together, the results showed that YbhFSR exhibited dual functions as a drug efflux pump and a Na + (Li +)/H + antiporter.

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Section: Discussionmentioning

confidence: 99%

A Putative Efflux Transporter of the ABC Family, YbhFSR, in Escherichia coli Functions in Tetracycline Efflux and Na+(Li+)/H+ Transport

et al. 2020

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Vaginal ecosystem modeling of growth patterns of anaerobic bacteria in microaerophilic conditions

et al. 2017

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The human vagina constitutes a complex ecosystem created through relationships established between host mucosa and bacterial communities. In this ecosystem, classically defined strict bacterial aerobes and anaerobes thrive as communities in the microaerophilic environment. Levels of CO2 and O2 present in the vaginal lumen are impacted by both the ecosystem’s physiology and the behavior and health of the human host. Study of such complex relationships requires controlled and reproducible causational approaches that are not possible in the human host that, until recently, was the only place these intact bacterial communities thrived. To address this need we have utilized our ex vivo human vaginal mucosa culture system to support controlled, reproducible colonization by vaginal microbiomes (VMB) collected from healthy and symptomatic donors. Parallel vaginal epithelial cells (VEC)-VMB co-cultures were exposed to increasingly microaerophilic conditions to study the impact of CO2 concentrations upon the anaerobic bacteria associated with dysbiosis and inflammation. Our data suggest that in the context of intact VMBs, increased CO2 concentrations favored specific lactobacilli species defined as aerobes or microaerophiles when grown as monocultures. The observed community changes also led to shifts in host VEC phenotypes with significant changes in the host transcriptome, including altered expression of select molecular transporter genes. These findings support the need for additional study of the environmental changes associated with behavior and health upon the symbiotic and adversarial relationships that are formed in microbial communities present in the human vaginal ecosystem.

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Dual-targeting hybrid peptide-conjugated doxorubicin for drug resistance reversal in breast cancer

Yuan

You

Chen

2016

International Journal of Pharmaceutics

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Multidrug resistance-associated ABC transporters – too much of one thing, good for nothing

Cited by 4 publications

References 101 publications

A Putative Efflux Transporter of the ABC Family, YbhFSR, in Escherichia coli Functions in Tetracycline Efflux and Na+(Li+)/H+ Transport

A Putative Efflux Transporter of the ABC Family, YbhFSR, in Escherichia coli Functions in Tetracycline Efflux and Na+(Li+)/H+ Transport

Vaginal ecosystem modeling of growth patterns of anaerobic bacteria in microaerophilic conditions

Dual-targeting hybrid peptide-conjugated doxorubicin for drug resistance reversal in breast cancer

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