Multidrug resistance in cancer (review)

Srivastava, Rakesh K.; Srivastava, Aparna R.; Ys, Cho-Chung

doi:10.3892/ijo.9.5.879

Cited by 8 publications

(9 citation statements)

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“…The drugs that are transported are large hydrophobic molecules and generally weak bases. The MDR of tumor cells has largely been attributed to overexpression of these``MDR'' proteins (see review by Srivastava et al 1996). The corresponding genes have been cloned, sequenced, and shown to be members of thè`A BC'' (ATP-binding cassette) or``trac ATPase'' superfamily.…”

Section: Introductionmentioning

confidence: 99%

Flow cytometry analysis of drug transport mechanisms in Haemonchus contortus susceptible or resistant to anthelmintics

Kerbœuf¹,

Chambrier²,

Vern³

et al. 1999

Parasitology Research

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The role of membrane drug-transport mechanisms in resistance to anthelmintics was examined using a flow cytometry method. This method was adapted from assays developed for the study of similar mechanisms in tumor cells. Rhodamine 123, a P-glycoprotein transport probe, associated with the reversal agent verapamil gave a significantly higher level of green fluorescence in Haemonchus contortus-resistant eggs as compared with that of susceptible eggs. In the same way, verapamilbodipy, a new fluorescent probe for the detection of multidrug resistance in cells, showed a significantly higher degree of binding to resistant eggs. The results confirm those obtained with biological drug assays using both anthelmintics and verapamil and provide a quantitative and effective methodology for the functional study of multidrug resistance in nematodes.

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Section: Introductionmentioning

confidence: 99%

Flow cytometry analysis of drug transport mechanisms in Haemonchus contortus susceptible or resistant to anthelmintics

Kerbœuf¹,

Chambrier²,

Vern³

et al. 1999

Parasitology Research

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“…Resistance of nematodes to such drugs has mainly been attributed to mechanisms associated with the binding of these molecules to speci®c intracellular targets (Martin et al 1997). However, in vertebrates, development of altered metabolism of xenobiotics greatly in¯uences the ecacy of a number of drugs and, in the case of antitumor agents, contributes to the development of resistance (see review by Srivastava et al 1996). It thus appears that metabolic reactions are also worthy of exploration in parasitic nematodes.…”

Section: Introductionmentioning

confidence: 97%

Unexpected increased thiabendazole tolerance in Haemonchus contortus resistant to anthelmintics by modulation of glutathione activity

Kerbœuf¹,

Aycardi²

1999

Parasitology Research

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The effect of several modulators of the synthesis or activity of glutathione (GSH) on the susceptibility of Haemonchus contortus eggs susceptible or resistant to anthelmintics was investigated using in vitro egg-hatch assays. Diethylmaleate, D,L-buthionine-[S,R]-sulfoximine, and patulin induced an unexpected decrease in the susceptibility of resistant eggs to thiabendazole, which was chosen as a reference for resistance to benzimidazole compounds. The results demonstrate that the level of GSH or SH analog plays an important role in the toxicity of thiabendazole to nematode eggs. Comparison with changes observed in the cytotoxicity of antitumor or antiprotozoal drugs after GSH modulation suggests that in H. contortus eggs this increased thiabendazole tolerance might depend on different factors, whether associated or not, including the ability of thiabendazole to conjugate with parasitic GSH or analog, the potential toxicity of such conjugates, their cellular distribution, and their role in the expression of glutathione S-transferase activities, and, perhaps, in the regulation of apoptosis.

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“…There are several cellular processes that contribute to both intrinsic and acquired chemoresistance (6). One of such processes is active extrusion of drugs from cells by ATP-binding cassette transporters (membrane proteins) (7). This process has low drug specificity and is termed multi-drug resistance (MDR).…”

Section: Introductionmentioning

confidence: 99%

Multi-Drug-Resistance in Drug-Naive and Drug-Exposed Ovarian Cancer Cell Lines Responds Differently to Cell Culture Dimensionality

Koshkin

Oliveira

Peng

et al. 2020

Preprint

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Does cell clustering in ovarian cancer influence intrinsic and acquired multi-drug resistance (MDR) differently? To address this question, we studied cultured monolayers (representing individual cells) and cultured spheroids (representing clusters) formed by drug-naive (intrinsic MDR) and drug-exposed (acquired MDR) lines of ovarian cancer A2780 cells by cytometry of reaction rate constant (CRRC). MDR efflux was characterized by accurate and robust "cell number vs. MDR efflux rate constant (kMDR)" histograms. Both drug-naive and drug-exposed monolayer cells presented unimodal histograms; the histogram of drug-exposed cells was shifted towards higher kMDR value suggesting greater MDR activity. Spheroids of drug-naive cells presented a bimodal histogram indicating the presence of two subpopulations with different MDR activity. In contrast, spheroids of drug-exposed cells presented a unimodal histogram qualitatively similar to that of the monolayers of drug-exposed cells but with a moderate shift towards greater MDR activity.

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Multidrug resistance in cancer (review)

Cited by 8 publications

References 0 publications

Flow cytometry analysis of drug transport mechanisms in Haemonchus contortus susceptible or resistant to anthelmintics

Flow cytometry analysis of drug transport mechanisms in Haemonchus contortus susceptible or resistant to anthelmintics

Unexpected increased thiabendazole tolerance in Haemonchus contortus resistant to anthelmintics by modulation of glutathione activity

Multi-Drug-Resistance in Drug-Naive and Drug-Exposed Ovarian Cancer Cell Lines Responds Differently to Cell Culture Dimensionality

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