1995
DOI: 10.1006/plas.1995.1034
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Multidrug Resistance Plasmid pSK108 from Coagulase-Negative Staphylococci; Relationships to Staphylococcus aureus qacC Plasmids

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Cited by 45 publications
(53 citation statements)
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“…This cassette feature appears to be quite widespread and could easily be overlooked, since common smr primers do not necessarily amplify the slightly larger additional PCR product in sufficient quantities. The MIC results in our study support previous findings that the partial smr sequence duplication is unlikely to mediate any significant effect on the resistance to QACs (13).…”
Section: Discussionsupporting
confidence: 91%
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“…This cassette feature appears to be quite widespread and could easily be overlooked, since common smr primers do not necessarily amplify the slightly larger additional PCR product in sufficient quantities. The MIC results in our study support previous findings that the partial smr sequence duplication is unlikely to mediate any significant effect on the resistance to QACs (13).…”
Section: Discussionsupporting
confidence: 91%
“…PCR detected four different QAC resistance genes ( Table 1). The gene smr was found in 21 bovine and six caprine isolates, and the qacCЈ sequence duplication was detected in 12 bovine and 2 caprine isolates; the presence of qacCЈ sequence was confirmed by partial plasmid sequencing and showed 100% identity to the corresponding sequence in pSK108 (13). Singlestrand primer walking sequencing of five selected plasmids resulted in complete plasmid sequences and identification of the two previously sequenced smr-and qacJ-containing plasmids pNVH99 (6) in S. aureus and pNVH01 (7) in S. hominis and S. delphini based on 99 to 100% sequence identity.…”
Section: Staphylococcal Isolatesmentioning
confidence: 83%
“…Sequence analysis of pNVH99 revealed 2,239 bp encoding two different open reading frames (ORFs). The 107-codon ORF (nucleotides [nt] 139 to 1682) is 99% similar at the nucleotide level with the previously sequenced smr genes in plasmids such as pST827, pSK41, pKH8, pSK89, pTZ22, and pSK108 (1,11,14,15,16,28). The smr gene in pNVH99 is characterized by one single nucleotide alteration (nt 1439, A replaced by G), without involving an amino acid change.…”
Section: Resultsmentioning
confidence: 63%
“…Due to the small size of the multidrug transporters of the SMR family, it has been proposed that they may function as homooligomeric complexes (187,189). The first gene encoding a multidrug transporter of the SMR family was detected on both conjugative and nonconjugative plasmids from clinical isolates of S. aureus and other staphylococci (120,121,132). This gene, first described as qacC and also known as qacD (132) and ebr (221), has been renamed smr (71).…”
Section: Small Multidrug Resistance Familymentioning
confidence: 99%