Multidrug-Resistant Avian Pathogenic<i>Escherichia coli</i>Strains and Association of Their Virulence Genes in Bangladesh

Saha, Otun; Hoque, M. Nazmul; Islam, Ovinu Kibria; Sultana, Munawar; Hossain, Mohammad Anwar

doi:10.1101/2020.06.30.180257

Cited by 17 publications

(31 citation statements)

References 76 publications

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“…Presumptive Micrococcus and Staphylococcus isolates were genotyped by Random Amplified Polymorphic DNA (RAPD) method using arbitrary primer 1283 (5′- GCGATCCCCA-3′) [ 24 ]. 16S rRNA genes of the representative isolates from each RAPD group were amplified by PCR using universal primers 27F (5′-AGAGTTTGATCCTGGCTCAG-3′) and 1492R (5′-CTACGGCTACCTTGTTACGA-3′) and subjected to sequencing [ 4 ]. DNA sequencing was carried out using Applied Biosystems highest capacity-based genetic analyzer (ABI PRISM® 377 DNA Sequencer) platforms with the BigDye® Terminator v3.1 cycle sequencing kit chemistry.…”

Section: Methodsmentioning

confidence: 99%

“…So, ensuring biosecurity for poultry is mandatory for economic prosperity and public health. But unfortunately, the manpower comprised of more than 3 million people associated directly with this sector [ 4 ] is mostly unskilled [ 5 ] and lacking proper knowledge of personal hygiene and sanitation strategies, thus risking this promising sector. Moreover, the lack of sufficient knowledge and training on the sanitation program is making the zoonotic disease transmission difficult to control/prevent leading to high health risks for both animals and farmers [ 6 ].…”

Section: Introductionmentioning

confidence: 99%

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Evaluation of Commercial Disinfectants against Staphylococcus lentus and Micrococcus spp. of Poultry Origin

Saha

Rakhi

Istiaq

et al. 2020

Veterinary Medicine International

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Introduction. Effective sanitation strategies for poultry farms require an appropriate selection of the disinfectant based on the contaminants present and their sensitivity to the disinfectants. Aim. The current study investigated the prevalence of streptococci/micrococci in poultry farms of Bangladesh and the efficacy of commercial disinfectants (Savlon, Lysol, Quatovet, Virkon S, and Virocid) along with alcohol against these pathogens to adopt appropriate strategies. Materials and Methods. Conventional approaches and the 16S rRNA gene sequencing were performed to confirm the isolates at the species level along with microtiter biofilm assay to determine their biofilm-forming ability. Efficacy of the disinfectants was tested against those isolates using agar well diffusion and minimum inhibitory concentration (MIC) test by broth dilution method using different dilutions of the disinfectants. Results. Staphylococcus lentus (n = 32), Micrococcus luteus (n = 7), and Micrococcus aloeverae (n = 4) were confirmed among 102 presumptively screened streptococci/micrococci isolates from 43 samples. No single disinfectant showed equally high efficacy against all three bacterial species in agar well diffusion test, although Virocid showed the lowest MIC against all three of them. Lysol was least effective among the commercial disinfectants by both MIC and diffusion method, although each commercial disinfectant was more effective than alcohol. Considering both the average diameter of the inhibition zones and the MIC values, efficacy can be interpreted as Virocid > Quatovet > Savlon > Virkon S > Lysol. Although the efficacy decreased with decreasing concentration, the disinfectants retained a satisfactory level of efficacy at 50% concentration. Among test pathogens, M. aloeverae was the most sensitive to the disinfectants and the weakest biofilm producers, whereas 4/14 S. lentus and 1/5 M. luteus were strong biofilm producers, which may cause more reduction in the efficacy in environmental conditions. Conclusion. As no ideal disinfectant was found in the study, the efficacy of the disinfectants should be routinely evaluated and validated to ensure the sanitation standards in the poultry sector.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Evaluation of Commercial Disinfectants against Staphylococcus lentus and Micrococcus spp. of Poultry Origin

Saha

Rakhi

Istiaq

et al. 2020

Veterinary Medicine International

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“…Numerous recent studies on serogroups, phylogenetic groups, and antimicrobial resistance in APEC isolates from chickens have been conducted in many countries [ 24 , 25 , 26 , 27 ], but such surveys are rarely performed on APEC isolates from ducks [ 28 , 29 ]. In Korea, the characteristics of APEC isolated from ducks have rarely been investigated as well [ 30 ], although consumption of duck meat has recently increased.…”

Section: Introductionmentioning

confidence: 99%

Comparative Characteristics and Zoonotic Potential of Avian Pathogenic Escherichia coli (APEC) Isolates from Chicken and Duck in South Korea

et al. 2021

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Avian pathogenic Escherichia coli (APEC) causes colibacillosis, which is an economically important disease in the poultry industry worldwide. The present study investigated O-serogroups, phylogenetic groups, antimicrobial resistance, and the existence of virulence-associated genes (VAGs) and antimicrobial resistance genes in 125 APEC isolates between 2018 and 2019 in Korea. The phylogenetic group B2 isolates were confirmed for human-related sequence types (STs) through multi-locus sequence typing (MLST). O-serogroups O2 (12.5%) and O78 (10.3%) and phylogenetic group B1 (36.5%) and A (34.5%) were predominant in chicken and duck isolates, respectively. Out of 14 VAGs, iucD, iroN, hlyF, and iss were found significantly more in chicken isolates than duck isolates (p < 0.05). The resistance to ampicillin, ceftiofur, ceftriaxone, and gentamicin was higher in chicken isolates than duck isolates (p < 0.05). The multidrug resistance (MDR) rates of chicken and duck isolates were 77.1% and 65.5%, respectively. One isolate resistant to colistin (MIC 16 μg/mL) carried mcr-1. The B2-ST95 APEC isolates possessed more than 9 VAGs, and most of them were MDR (82.4%). This report is the first to compare the characteristics of APEC isolates from chickens and ducks in Korea and to demonstrate that B2-ST95 isolates circulating in Korea have zoonotic potential and pose a public health risk.

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“…Immune response in poultry against APEC is directly related to "O" antigens (Nolan et al, 2013). Several virulence factors have been associated with the pathogenicty of APEC including haemolysin, adhesin (fim), toxins, invasins, capsule as well as lipopolysaccharide complex (Ewers et al, 2003;Saha et al, 2020). However, several studies have demonstrated that all virulence factors are rarely present in same isolate.…”

Section: Introductionmentioning

confidence: 99%

Studies of Virulence Factors of Avian Pathogenic Escherichia coli in Avian Colibacillosis by In vitro Method and PCR

Singh¹,

Chhabra²,

Gangil³

et al. 2021

IJAR

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Background: Avian colibacillosis is considered as major cause of morbidity and mortality in poultry. It is a common bacterial disease of poultry and many virulence factors of E. coli are associated with the disease. The current study was aimed to investigate the presence of some virulence factors of E. coli isolated from the cases of colibacillosis.Methods: In present study, total 150 samples (liver, heart, lungs, air sacs and feaces) of chicken exhibiting pathological conditions of colibacillosis were collected from various poultry farms (organized and backyard) situated in and around Mhow and Indore cities. E.coli was isolated and identified from the samples on the basis of cultural characteristics and biochemical test. All E. coli isolates were further subjected to evaluate the presence of virulence factors such as biofilm production, haemolysis, invasiveness and molecular detection of fimH and stx1 gene.Result: Out of these 51.33% of incidence of E. coli was recorded. E. coli O84 and O149 serotypes were found most prevalent. Out of 77 isolates, 46 (59.7%) and 45 (58.4%) were positive for biofilm formation by tube method and modified CRA method, respectively. All E. coli isolates were showing invasiveness in congo red binding assay while none of the isolates was found haemolytic. Molecular detection revealed the presence of fimH (508bp) gene in 33.3% of tested samples while stx1 gene could not be detected in any isolates.

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Multidrug-Resistant Avian PathogenicEscherichia coliStrains and Association of Their Virulence Genes in Bangladesh

Cited by 17 publications

References 76 publications

Evaluation of Commercial Disinfectants against Staphylococcus lentus and Micrococcus spp. of Poultry Origin

Evaluation of Commercial Disinfectants against Staphylococcus lentus and Micrococcus spp. of Poultry Origin

Comparative Characteristics and Zoonotic Potential of Avian Pathogenic Escherichia coli (APEC) Isolates from Chicken and Duck in South Korea

Studies of Virulence Factors of Avian Pathogenic Escherichia coli in Avian Colibacillosis by In vitro Method and PCR

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