Multidrug-resistant coagulase-negative Staphylococci in food animals

Abstract

Aims To study the antimicrobial resistance of coagulase‐negative staphylococci (CoNS) in animals. Methods and Results In the present study, a total of 87 CoNS recovered from food animals were characterized by antimicrobial susceptibility testing, resistance gene identification and conjugation. Of the seven species studied, Staphylococcus lentus, Staphylococcus sciuri, Staphylococcus xylosus and Staphylococcus haemolyticus accounted for over 96% of the isolates. In addition to β‐lactam resistance (100%), high percentages of CoNS were resistant to tetracycline (67·8%), erythromycin (36·7%), clindamycin (27·5%) and quinopristin/dalfopristin (14·9%). Importantly, 47 (54%) isolates were resistant to at least three antimicrobial classes, including six CoNS resistant to six antimicrobial classes. The common genes for the above‐mentioned resistance phenotypes were mec(A), tet(M), erm(A) and vga(A)LC, which were identified from 68·7%, 61%, 56·2% and 69·2% of the isolates, respectively. tet(M) was conjugatively transferable from 10 tetracycline‐resistant CoNS to a Enterococcus strain, underlining the potential of antimicrobial resistance transfer from Staphylococcus to the commensal bacteria in human. Conclusions Multidrug resistance and resistance to non‐β‐lactam antimicrobials are common in CoNS in animals. Significance and Impact of the Study The data improve our understanding on the extent to which CoNS contribute to the dissemination of antimicrobial resistance in the food production environment.

Antimicrobial resistance and population structure of Staphylococcus epidermidis recovered from pig farms in Belgium

“…In other published studies, MRSE has been infrequently or not detected in farmed pigs (Zhang et al, 2009;Bhargava and Zhang, 2012). Similarly, in poultry (Zhang et al, 2009;Bhargava and Zhang, 2012) and cattle relatively few MRSE isolates have been identified in healthy populations.…”

“…In other published studies, MRSE has been infrequently or not detected in farmed pigs (Zhang et al, 2009;Bhargava and Zhang, 2012). Similarly, in poultry (Zhang et al, 2009;Bhargava and Zhang, 2012) and cattle relatively few MRSE isolates have been identified in healthy populations. Conversely, S. epidermidis is one of the main CoNS causing ruminant intramammary infections (Vanderhaeghen et al, 2014), with similar methicillin-resistance rates as reported in the current study (Sampimon et al, 2011;Frey et al, 2013).…”

Retail Ready-to-Eat Food as a Potential Vehicle for Staphylococcus spp. Harboring Antibiotic Resistance Genes

“…Most of the previous studies on resistance have concentrated on staphylococci isolated from clinical samples (26,31). Recently, some researchers have suggest ed that food could be an appropriate environment for resistant and multiresistant strains and that the food chain could play a key role in the transmission of resistance between the environment and humans (3,12,20,22). This study gives a better understanding of the multiple antimicrobial resistance profiles observed in staphylococcal isolates from RTE food in Poland.…”

Diversity of antimicrobial resistance and virulence genes in methicillin-resistant non-Staphylococcus aureus staphylococci from veal calves

“…As problems with breakpoints for NAS have been noted before (Nemeghaire et al, 2014b(Nemeghaire et al, , 2014c, it may be necessary to evaluate the breakpoints at staphylococcal species level. Most genes have already been described among NAS of animal origin (Bhargava and Zhang, 2012;Chah et al, 2014;Frey et al, 2013;Kern and Perreten, 2013;Nemeghaire et al, 2014bNemeghaire et al, , 2014cWeiß et al, 2014;Wendlandt et al, 2013). The presence of other genes or combination of genes was particularly relevant in certain species.…”