Multidrug-resistant Enterobacterales responsible for septicaemia in a neonatal intensive care unit in Morocco

Pérez‐Palacios, Patricia; Girlich, Delphine; Soraa, Nabila; Hanchi, Asmae Lamrani; Maoulainine, Fadl Mrabih Rabo; Bennaoui, Fatiha; Amri, Hasna; Idrissi, Nadia Slitine El; Bouskraoui, M.; Birer, Alain; Jousset, Agnès B; Oueslati, Saoussen; Raymond, Josette; Naas, Thierry

doi:10.1016/j.jgar.2023.02.011

Cited by 13 publications

(11 citation statements)

References 37 publications

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Section: Discussionmentioning

confidence: 89%

“…Based on the observed results, the significance of hygiene measures in NICUs is highlighted in our study [ 30 ]. These measures are essential for preventing the emergence and transmission of MDR bacteria [ 30 , 31 ]. It is crucial to establish systematic surveillance systems and employ effective methods for detecting antimicrobial susceptibility as a future prospective.…”

Section: Discussionmentioning

confidence: 89%

“…The treatment of infections in neonates caused by MDR Enterobacterales harboring ESBLs is challenging due to the limited availability of antibiotics. Colistin, fluoroquinolones, and tigecycline are not commonly used in this population due to their side effects [ 30 ]. The emergence of antibiotic-resistant pathogens is closely associated with the inappropriate and excessive use of broad-spectrum antibiotics.…”

Section: Discussionmentioning

confidence: 99%

“…It is crucial to establish systematic surveillance systems and employ effective methods for detecting antimicrobial susceptibility as a future prospective. This will enhance antibiotic stewardship and ensure adherence to infection control measures, such as hand hygiene and surface cleaning [ 30 , 31 ].…”

Section: Discussionmentioning

confidence: 99%

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High prevalence of multidrug-resistant Enterobacterales carrying extended-spectrum beta-lactamase and AmpC genes isolated from neonatal sepsis in Ahvaz, Iran

Mansouri,

Savari,

Malakian

et al. 2024

BMC Microbiol

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Objectives In the recent years, multidrug resistant (MDR) neonatal septicemia-causing Enterobacterales has been dramatically increased due to the extended-spectrum beta-lactamases (ESBLs) and AmpC enzymes. This study aimed to assess the antibiotic resistance pattern, prevalence of ESBLs/AmpC beta-lactamase genes, and Enterobacterial Repetitive Intergenic Consensus Polymerase Chain Reaction (ERIC-PCR) fingerprints in Enterobacterales isolated from neonatal sepsis. Results In total, 59 Enterobacterales isolates including 41 (69.5%) Enterobacter species, 15 (25.4%) Klebsiella pneumoniae and 3 (5.1%) Escherichia coli were isolated respectively. Resistance to ceftazidime and cefotaxime was seen in all of isolates. Furthermore, all of them were multidrug-resistant (resistant to three different antibiotic categories). The phenotypic tests showed that 100% of isolates were ESBL-positive. Moreover, AmpC production was observed in 84.7% (n = 50/59) of isolates. Among 59 ESBL-positive isolates, the highest percentage belonged to blaCTX−M−15 gene (66.1%) followed by blaCTX−M (45.8%), blaCTX−M−14 (30.5%), blaSHV (28.8%), and blaTEM (13.6%). The frequency of blaDHA, blaEBC, blaMOX and blaCIT genes were 24%, 24%, 4%, and 2% respectively. ERIC-PCR analysis revealed that Enterobacterales isolates were genetically diverse. The remarkable prevalence of MDR Enterobacterales isolates carrying ESBL and AmpC beta-lactamase genes emphasizes that efficient surveillance measures are essential to avoid the more expansion of drug resistance amongst isolates.

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Section: Discussionmentioning

confidence: 89%

Section: Discussionmentioning

confidence: 89%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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High prevalence of multidrug-resistant Enterobacterales carrying extended-spectrum beta-lactamase and AmpC genes isolated from neonatal sepsis in Ahvaz, Iran

Mansouri,

Savari,

Malakian

et al. 2024

BMC Microbiol

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“…The minimum inhibitory concentrations (MICs) of beta-Lactams, ciprofloxacin, levofloxacin, and tobramycin were determined and interpreted using the EUCAST guidelines. The detection of a carbapenem-hydrolysis was carried out using the homemade Carba NP, and the presence of one of the 5 main carbapenemases was confirmed by the NG-Test CARBA 5 Lateral Flow ImmunoAssay (NG Biotech, Guipry, France) as previously described [ 16 ].…”

Section: Methodsmentioning

confidence: 99%

Antibiotic Susceptibility Profiles of Bacterial Isolates Recovered from Abscesses in Cattle and Sheep at a Slaughterhouse in Algeria

Yousfi,

Oueslati,

Daaboul

et al. 2024

Microorganisms

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Abscesses represent the most prominent emerging problem in the red meat industry, leading to great economic constraints and public health hazards. Data on etiological agents present in these purulent lesions in Algeria are very scarce. The aim of this study was to identify the bacteria responsible for these abscesses and to determine their antibiotic susceptibility profiles. A total of 123 samples of abscesses from 100 slaughtered sheep and 23 slaughtered cattle were cultured in several media. A total of 114 bacterial isolates were cultured from 103 abscesses. Bacteria were identified using MALDI–TOF, and antibiotic susceptibility was determined by the disk diffusion method on Mueller–Hinton agar. A total of 73.6% (n = 84) corresponded to Enterobacterales, of which four were multidrug-resistant (MDR). These isolates, together with Staphylococcus aureus, coagulase negative Staphylococci, and seven randomly chosen susceptible Escherichia coli isolates, were further characterized using WGS. Resistome analysis of the four MDR Enterobacterales isolates revealed the presence of OXA-48 carbapenemase in two Klebsiella pneumoniae ST985 and one E. coli ST10 isolates and a CTX-M-15 ESBL in one E. coli isolate ST1706. Two coagulase-negative Staphylococci isolates were found to carry the mecA gene. WGS showed the presence of different resistance genes and virulence genes. Our study revealed 5% of MDR Enterobacterales (including ESBLs and carbapenemases) identified from abscesses, thus urging the need for abscess monitoring in slaughterhouses.

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Challenges and Trends in Gram-Negative Bacterial Infections in Critically Neonates: A Seven-and-a-Half-Year Observational Study

da Silveira Ferreira,

de Brito Machado,

de Paula Menezes

et al. 2024

American Journal of Infection Control

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Multidrug-resistant Enterobacterales responsible for septicaemia in a neonatal intensive care unit in Morocco

Cited by 13 publications

References 37 publications

High prevalence of multidrug-resistant Enterobacterales carrying extended-spectrum beta-lactamase and AmpC genes isolated from neonatal sepsis in Ahvaz, Iran

High prevalence of multidrug-resistant Enterobacterales carrying extended-spectrum beta-lactamase and AmpC genes isolated from neonatal sepsis in Ahvaz, Iran

Antibiotic Susceptibility Profiles of Bacterial Isolates Recovered from Abscesses in Cattle and Sheep at a Slaughterhouse in Algeria

Challenges and Trends in Gram-Negative Bacterial Infections in Critically Neonates: A Seven-and-a-Half-Year Observational Study

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