Multidrug-Resistant Enteropathogenic Escherichia coli Isolated from Diarrhoeic Calves, Milk, and Workers in Dairy Farms: A Potential Public Health Risk

Eldesoukey, Ibrahim E.; Elmonir, Walid; Alouffi, Abdulaziz; Beleta, Eman I. M.; Kelany, Mohamed A.; Elnahriry, Shimaa S.; Alghonaim, Mohammed I.; Alzeyadi, Zeyad Abdullah; Elaadli, Haitham

doi:10.3390/antibiotics11080999

Cited by 9 publications

(7 citation statements)

References 35 publications

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“…No. K001-1KT; HiMedia, Maharashtra, India) ( 9 , 27 ). Biochemical analysis confirmed the presence of pure E. coli colonies by means of triple sugar iron agar (TSIA) (cat.…”

Section: Methodsmentioning

confidence: 99%

“…The spread and transfer of E. coli with ESBLs can take place through food supply chains, contaminated faeces, contaminated water, and hazardous waste. Clinical symptoms or disorders caused by E. coli can appear as urinary tract infections, septic shock, and diarrhoeal illnesses ( 9 , 18 ). Mastitis is a condition that can affect lactating animals and has been linked to E. coli infections ( 3 , 12 ).…”

Section: Introductionmentioning

confidence: 99%

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Molecular identification of blaTEM and blaCTX-M genes in multidrug-resistant Escherichia coli found in milk samples from dairy cattle farms in Tulungagung, Indonesia

Widodo,

Lamid,

Effendi

et al. 2023

Journal of Veterinary Research

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Introduction Escherichia coli is an opportunistic bacteria that can grow easily, produce toxins, and resist antibiotics. The phenomenon of E. coli developing multidrug resistance is currently the subject of extensive research. The objective of this study was to molecularly identify blaTEM and blaCTX-M genes in multidrug-resistant E. coli found in milk samples from dairy cattle farms in Tulungagung, Indonesia. Material and Methods One hundred and ten milk samples were collected from 45 dairy cattle farms in Tulungagung, Indonesia. Indole, methyl red, Voges–Proskauer and in citrate tests and triple iron sugar agar tests were used to identify E. coli. Multidrug resistance was determined in isolates through antibiotic sensitivity tests using tetracycline, streptomycin, trimethoprim, chloramphenicol and aztreonam. Extended-spectrum beta lactamase enzyme production was confirmed by double-disc synergy test (DDST). Molecular identification was performed to confirm the blaTEM and blaCTX-M genes. Results One hundred and one (91.82%) E. coli strains were isolated from the samples. The antibiotic sensitivity test showed four (3.96%) multidrug-resistant (MDR) and one (0.99%) ESBL-positive E. coli by DDST confirmation. There were three (77.78%) blaTEM genes and one (0.99%) blaCTX-M gene discovered in the MDR E. coli isolates using PCR for molecular identification. Conclusion The findings of the blaTEM and blaCTX-M genes encoding ESBL E. coli in dairy cattle milk in Tulungagung, Indonesia is concerning and argues for prompt action to stop the emergence of antibiotic resistance which has an impact on public health.

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“…No. K001-1KT; HiMedia, Maharashtra, India) ( 9 , 27 ). Biochemical analysis confirmed the presence of pure E. coli colonies by means of triple sugar iron agar (TSIA) (cat.…”

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Molecular identification of blaTEM and blaCTX-M genes in multidrug-resistant Escherichia coli found in milk samples from dairy cattle farms in Tulungagung, Indonesia

Widodo,

Lamid,

Effendi

et al. 2023

Journal of Veterinary Research

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“…In Egypt, a study of Enteropathogenic E. coli (EPEC) either isolated from diarrhoeic calves (17/75), milk (8/150), or workers (3/15) in dairy farms has reported that all EPEC (100%) isolates were MDR, with high resistance rates observed in ampicillin (100%), tetracycline (89.3%), cefazolin (71%), and ciprofloxacin (64.3%) [25].…”

Section: Current Situation Of E Coli Antibiotic Resistance In Food-pr...mentioning

confidence: 99%

Current Situation of Escherichia coli Antibiotic Resistance in Food-producing Animals, Wild Animals, Companion Animals, and Birds: One Health Perspectives

Ishag¹,

Abdelwahab²,

Hammadi³

et al. 2022

Escherichia Coli Infections - An Update [Working Title]

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Escherichia coli (E. coli) has shown antimicrobial resistance (AMR) to a range of antibiotics, including the last resort antibiotics, which represent a global burden. Thus, it is essential to consider the AMR from a One Health perspective due to the ability of antimicrobial resistance to be transmitted between animals and humans share the same environment. As a result, and to minimize the emergence and spread of the AMR requires coordination in the multi-sectorial effort. However, in most cases, animals and birds have been ignored by public health authorities while antimicrobial resistance is being reported. This gap poses a serious public health burden due to the close contact between food-producing animals, companion animals, or companion birds, humans, and their environment. Therefore, this chapter aims to highlight the current situation of E. coli AMR in food-producing animals, wild animals, companion animals, and birds from One Health perspective. In conclusion, the chapter shows that E. coli exerted resistance to a range of antibiosis, including the last resort of antibiotics in livestock and birds worldwide which calls for joint efforts under one health umbrella to address the challenge of E. coli AMR in animals and birds.

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“…Manifestations of disease in animals caused by these bacteria result in mastitis (Aslam et al 2021). Other diseases are digestive diseases in animals and humans which are often caused by E. coli (EPEC) strains (Eldesoukey et al 2022), urinary traTable 1ct diseases and septic shock in humans (Mueller and Tainer 2022).…”

Section: Introductionmentioning

confidence: 99%

Antimicrobial resistance characteristics of multidrug resistance and extended-spectrum beta-lactamase producing Escherichia coli from several dairy farms in Probolinggo, Indonesia

et al. 2023

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Abstract. Widodo A, Lamid M, Effendi MH, Khailrullah AR, Kurniawan SC, Silaen OSM, Riwu KHP, Yustinasari LR, Afnani DA, Dameanti FNAEP, Ramandinianto SC. 2022. Antimicrobial resistance characteristics of multidrug resistance and extended spectrum beta-lactamase producing Escherichia coli from several dairy farms in Probolinggo, Indonesia. Biodiversitas 23: 215-221. Escherichia coli bacteria initially reside in the digestive tract of humans and animals but are able to adapt to new environments that are different from their initial habitat. The pathogenicity of E. coli can occur when these bacteria grow more than normal limits, produce toxins, and are resistant to certain types of antibiotics. The purpose of this study was to investigate the antimicrobial resistance characteristics of MDR and ESBL-producing E. coli from several dairy farms in the Probolinggo district of East Java province, Indonesia. A total of 150 samples consisting of 109 milk and 41 environmental samples from 41 dairy farms were used for isolation. TSIA and IMViC biochemical tests were used to identify E. coli bacteria. Escherichia coli resistance profile was obtained through disc diffusion test on several antibiotics, namely tetracycline, streptomycin, trimethoprim, chloramphenicol and aztreonam. Escherichia coli that was resistant to 3 or more antibiotics was defined as MDR. The results of isolation and identification obtained 124 (82.6%) isolates characterizing E. coli bacteria. The antimicrobial susceptibility test of E. coli showed 9 (7.26%) MDR isolates and 2 (22.22%) ESBL isolates by double-disc synergy test (DDST). MDR E. coli was dominated by the pattern of antimicrobial drug resistance TE-S-W (tetracycline, streptomycin, trimethoprim) with a total of 8 (38.10%) isolates, followed by antimicrobial drug resistance pattern TE-S-W-ATM (tetracycline, streptomycin, trimethoprim, aztreonam) with one (4.76%) E. coli isolates. The pattern of antimicrobial drugs of ESBL E. coli showed in one (11.11%) sample of ESBL E. coli from a milk sample with the pattern of TE-S-W-ATM (tetracycline, streptomycin, trimethoprim, aztreonam) and one (11.11%) sample ESBL E. coli (AL 30) from the environmental sample with a pattern of TE-S-W pattern (tetracycline, streptomycin, trimethoprim). The discovery of MDR E. coli isolates and ESBL E. coli from milk and environmental samples at several dairy farms in Probolinggo district, East Java, Indonesia is a matter of concern and requires real action to reduce antibiotic resistance.

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Multidrug-Resistant Enteropathogenic Escherichia coli Isolated from Diarrhoeic Calves, Milk, and Workers in Dairy Farms: A Potential Public Health Risk

Cited by 9 publications

References 35 publications

Molecular identification of blaTEM and blaCTX-M genes in multidrug-resistant Escherichia coli found in milk samples from dairy cattle farms in Tulungagung, Indonesia

Molecular identification of blaTEM and blaCTX-M genes in multidrug-resistant Escherichia coli found in milk samples from dairy cattle farms in Tulungagung, Indonesia

Current Situation of Escherichia coli Antibiotic Resistance in Food-producing Animals, Wild Animals, Companion Animals, and Birds: One Health Perspectives

Antimicrobial resistance characteristics of multidrug resistance and extended-spectrum beta-lactamase producing Escherichia coli from several dairy farms in Probolinggo, Indonesia

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