Multidrug-Resistant Escherichia albertii: Co-occurrence of β-Lactamase and MCR-1 Encoding Genes

Li, Qun; Wang, Hong; Xu, Yun; Bai, Xiangning; Wang, Jianping; Zhang, Zhengdong; Liu, Xiang; Miao, Yimao; Zhang, Ling; Li, Xinqiong; Zou, Nianli; Yan, Guodong; Chen, Xi; Zhang, Jie; Fu, Songzhe; Fan, Ruyue; Xu, Jianguo; Li, Juan; Xiong, Yanwen

doi:10.3389/fmicb.2018.00258

Cited by 25 publications

(26 citation statements)

References 44 publications

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“…We found that MPE isolates carried other antimicrobial resistance genes, especially ESBLs, ampC, and carbapenemase genes; particularly in the IncHI2A-type mcr-1 -harboring plasmids, multiple resistance genes were identified. This was in agreement with previous studies ( Mediavilla et al, 2016 ; Bi et al, 2017 ; Rhouma and Letellier, 2017 ; Li Q. et al, 2018 ; Zheng et al, 2019 ). A study in China investigated the coexistence of ESBLs and mcr-1 genes in E. coli isolated from chickens between 2008 and 2014 and concluded that mcr-1 -positive strains carry at least one ESBL ( Wu et al, 2018 ).…”

Section: Discussionsupporting

confidence: 94%

Antimicrobial Susceptibility and Virulence of mcr-1-Positive Enterobacteriaceae in China, a Multicenter Longitudinal Epidemiological Study

Jiang

Jia

et al. 2020

Front. Microbiol.

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This study was to investigate the prevalence of mcr-1 -positive Enterobacteriaceae (MPE) in intra-abdominal infections (IAIs), urinary tract infections (UTIs), and lower respiratory tract infections (LRTIs) in China. A total of 6,401 Enterobacteriaceae isolates were collected consecutively from IAI, UTI, and LRTI patients in 19 hospitals across mainland China during 2014–2016. MPE isolates were screened by PCR detection for the mcr gene. The resistance profiles were tested by antimicrobial susceptibility test. All MPE isolates were characterized by pulsed-field gel electrophoresis (PFGE), multi-locus-sequence typing, O and H serotyping, and whole-genome sequencing. Among the 6,401 Enterobacteriaceae isolates, 17 Escherichia coli strains (0.27%) were positive for the mcr-1 gene. The MPE prevalence rates in IAI, UTI, and LRTI patients were 0.34% (12/3502), 0.23% (5/2154), and 0% (0/745), respectively. The minimum inhibition concentrations (MICs) of colistin against 3 isolates were of 0.5–2 mg/L, and 4–8 mg/L against other 14 isolates. All the 17 isolates were susceptible to meropenem, imipenem, tigecycline, and ceftazidime/avibactam. The 17 MPE isolates belonged to 14 different ST types, and those that belonged to the same STs were not clonal by PFGE. The mcr-1 -harboring plasmid of ten MPE isolates could transfer to the recipients by conjugation and the colistin MICs of the transconjugants ranged from 0.5 to 8 mg/L. M cr-1 -carrying plasmids from the 17 MPE isolates could be grouped into four clusters, including 8 IncX4 type, 4 IncI2 type, 4 IncHI2A type, and 1 p0111 type. Multiple-drug resistance genes and virulence genes were detected. In conclusion, the prevalence of MPE in IAI, UTI, and LRTI were low in China, and no clonal transmission was identified in our study. Most MPE isolates exhibited low-level colistin resistance. However, our study indicated that MPE isolates always carried a variety of drug resistance and virulence genes, which should be paid more attention.

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Section: Discussionsupporting

confidence: 94%

Antimicrobial Susceptibility and Virulence of mcr-1-Positive Enterobacteriaceae in China, a Multicenter Longitudinal Epidemiological Study

Jiang

Jia

et al. 2020

Front. Microbiol.

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“…In countries such as Japan and Norway, the number of infections caused by E. albertii is increasing, which is a warning sign that the bacterium is causing problems around the world (24). E. albertii is important not only for its pathogenicity and its role in gastroenteritis in the world but also for its resistance to certain antibiotics (6,7). Therefore, it is important to continuously examine patients with diarrhea and urinary tract infections to detect E. albertii.…”

Section: Discussionmentioning

confidence: 99%

“…Its misdiagnosis as Enteropathogenic Escherichia Coli (EPEC), Enterohemorrhagic Escherichia coli (EHEC), or other pathogenic bacteria has continued from the past to the present. Recently, its pathogenic potential has been strengthened by identifying strains with multiple antibiotic resistance (5)(6)(7).…”

Section: Introductionmentioning

confidence: 99%

Detection of Escherichia albertii in urinary and gastrointestinal infections in Kermanshah, Iran

Namdari¹,

Rahimian-Zarif²,

Foroughi³

2020

Preprint

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Background Escherichia albertii (E. albertii) is a Gram-negative and facultative anaerobe bacterium. In recent years, the bacterium has been isolated from the feces of people with gastroenteritis as a pathogen that causes diarrhea. Due to insufficient information on the phenotypic and biochemical characteristics of E. albertii, it is difficult to distinguish it from other species of the Enterobacteriaceae family. This is especially prevalent in the pathotypes of Escherichia coli (E. coli). Moreover, in clinical laboratories, it is mistakenly identified as E. coli or even Hafnia alvei (H. alvei). This study was performed for the first time in Iran to identify E. albertii by PCR method from a sample of urinary and gastrointestinal infections obtained from clinical laboratories in Kermanshah, which were distinguished as E. coli. Methods In this study, 60 urinary samples and 40 fecal samples that were identified as E. coli by phenotypic and biochemical methods in clinical laboratories. The samples were re-evaluated in the first step in terms of specific phenotypic and biochemical characteristics of E. coli. Subsequently, DNA was extracted from the isolates by the phenol method. Then, two lysP and mdh genes were detected for E. albertii and the uidA gene for E. coli by PCR using specific primers pairs. Results The results obtained from phenotypic and biochemical tests indicated that all samples were consistent with E. coli characteristics. However, findings from PCR showed that out of a total of 100 samples, specific genes of E. coli were identified in 6 samples (6%) and uidA gene in 94 remaining samples (94%). Of these 6 samples, 5 samples were urinary tract infections, and only one was a gastrointestinal infection. Conclusion The findings of this study show that E. albertii can be considered as one of the causes of urinary and gastrointestinal infections that are mistakenly identified in clinical laboratories as E. coli.Therefore, the use of molecular methods for accurate and definitive diagnosis of bacteria can be useful.

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“…Furthermore, the recently discovered plasmid‐born mcr‐1 already is found on extended‐spectrum β‐lactamase multidrug resistance plasmids (e.g. Li et al , ), while plasmid‐mediated multidrug efflux cassettes are also linked to multidrug‐resistant infections in the hospital setting (Kim et al , ). While it is shown that originally these resistance elements have been selected due to a direct effect of polymyxin and olaquindox to promote animal growth their enrichment in the animal gut in response to florfenicol, a non‐cognate class of antibiotic was alarming.…”

Section: Discussionmentioning

confidence: 99%

Preventive antibiotic treatment of calves: emergence of dysbiosis causing propagation of obese state‐associated and mobile multidrug resistance‐carrying bacteria

Dobrzanska

Lamaudière

Rollason

et al. 2019

Microbial Biotechnology

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Summary In agriculture, antibiotics are used for the treatment and prevention of livestock disease. Antibiotics perturb the bacterial gut composition but the extent of these changes and potential consequences for animal and human health is still debated. Six calves were housed in a controlled environment. Three animals received an injection of the antibiotic florfenicol (Nuflor), and three received no treatment. Faecal samples were collected at 0, 3 and 7 days, and bacterial communities were profiled to assess the impact of a therapy on the gut microbiota. Phylogenetic analysis (16S‐rDNA) established that at day 7, antibiotic‐treated microbiota showed a 10‐fold increase in facultative anaerobic Escherichia spp, a signature of imbalanced microbiota, dysbiosis. The antibiotic resistome showed a high background of antibiotic resistance genes, which did not significantly change in response to florfenicol. However, the maintenance of Escherichia coli plasmid‐encoded quinolone, oqxB and propagation of mcr‐2, and colistin resistance genes were observed and confirmed by Sanger sequencing. The microbiota of treated animals was enriched with energy harvesting bacteria, common to obese microbial communities. We propose that antibiotic treatment of healthy animals leads to unbalanced, disease‐ and obese‐related microbiota that promotes growth of E. coli carrying resistance genes on mobile elements, potentially increasing the risk of transmission of antibiotic resistant bacteria to humans.

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Multidrug-Resistant Escherichia albertii: Co-occurrence of β-Lactamase and MCR-1 Encoding Genes

Cited by 25 publications

References 44 publications

Antimicrobial Susceptibility and Virulence of mcr-1-Positive Enterobacteriaceae in China, a Multicenter Longitudinal Epidemiological Study

Antimicrobial Susceptibility and Virulence of mcr-1-Positive Enterobacteriaceae in China, a Multicenter Longitudinal Epidemiological Study

Detection of Escherichia albertii in urinary and gastrointestinal infections in Kermanshah, Iran

Preventive antibiotic treatment of calves: emergence of dysbiosis causing propagation of obese state‐associated and mobile multidrug resistance‐carrying bacteria

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