Multidrug-resistant Gram-negative bacterial colonization in patients, carriage by healthcare workers and contamination of hospital environments in Ghana

Abstract

Purpose Patients already colonized with multidrug-resistant (MDR) Gram-negative bacteria (GNB) on admission to critical care units may be an important source of their transmission in hospitals. We sought to determine the prevalence of MDR GNB colonization in patients, staff, and the ward environment and to assess the risk factors for colonization of patients. Methods The study was conducted from April 2021 to July 2021 in a teaching hospital in Ghana. MDR GNB were isolated from rectal and hand swabs of patients on admission and after 48 hours. Swabs from HCW’s hands and the ward environment were also taken. Risk factors for colonization with MDR GNB were assessed using univariate and multivariate analysis. Results MDR GNB rectal colonization rate among patients was 50.62% on admission and 44.44% after 48 hours. MDR GNB were isolated from 6 (5.26%) and 24 (11.54%) of HCW’s hand swabs and environmental swabs, respectively. Escherichia coli and Klebsiella pneumoniae were the most frequently isolated MDR. Previous hospitalization (p-value = 0.021, OR,95% CI = 7.170 (1.345–38.214) was significantly associated with colonization by MDR GNB after 48 hours of admission. Age (21–30 years) (p-value = 0.022, OR, 95% CI = 0.103(0.015–0.716) was significantly identified as a protective factor associated with a reduced risk of rectal MDR GNB colonization. Conclusions The high colonization of MDR GNB in patients and carriage of MDR GNB on HCW’s hands and contamination of environments, highlights the need for patient screening, and stringent infection prevention and control practices to prevent the spread of MDR GNB in hospitals.