Multidrug-Resistant <b><i>Escherichia coli</i></b> Strains Isolated from Patients Are Associated with Class 1 and 2 Integrons

Abstract

Background:Escherichia coli isolates displaying multidrug-resistance (MDR) are a major health care problem that results in mortality and morbidity. Integrons are DNA elements in E.coli that are related to antibiotic resistance. The aim of this study was to determine class 1 and 2 integrons and MDR in E. coli isolates obtained from patients in two Sanandaj hospitals, located in Iran. Materials and Methods: 120 isolates of E. coli were obtained from clinical specimens (from November 2013 to April 2014), and the susceptibility of E. coli antimicrobial agents was determined using the Kirby-Bauer disk diffusion method according to the CLSI. PCR were applied for detection of class 1 and 2 integronsinE. coliisolates. SPSS software v16 and the χ² test were used for statistical analysis in order to calculate the association between antibiotic resistance and the presence of integrons (p < 0.05). Results: In a total of 120 E. coli isolates, 42.5% had MDR. Integrons were found in 50.9% of the MDR isolates, and included 47.05% class 1 and 3.92% class 2 integrons. The strains did not have both classes of integrons simultaneously. An association between resistance to antibiotics and integrons was found. Conclusion: Our results showed that int1 and int2 genes present in E. coli isolates obtained from patients cause MDR in this isolates. Since such bacteria are a reservoir for the transmission of MDR bacteria, appropriate programs are necessary to reduce this problem.

Resistance genes to sulphonamide in commensal Escherichia coli isolated from stool of patients in Mansoura University Children Hospital

“…This study assessed the presence of int1 gene among the studied isolates, and detected the gene in 28 (62%) of them. These results approximates the finding of Infante et al (2005), who found int1 gene in 9 (45%) of their studied 20 isolates and Lavakhamseh et al (2016) who recorded the presence of the same gene in 47% of their studied isolates. On the other hand, higher percentage of int1 gene (95%) was recorded by Frank et al (2007).…”

High Prevalence of Antimicrobial Resistance and Integron Gene Cassettes in Multi-Drug-Resistant Klebsiella pneumoniae Isolates From Captive Giant Pandas (Ailuropoda melanoleuca)

“…The intI2 and intI3 genes were not detected in our study. Many studies revealed that the class 1 integron was more prevalent than the class 2 or 3 in gram-negative bacteria (Lavakhamseh et al, 2016;Chamosa et al, 2017;Rehman et al, 2017;Sidhu et al, 2017). Moreover, class 3 integrons are reported to be rare in animals and clinical samples.…”

Extended-Spectrum Beta-Lactamases Producing Pseudomonas aeruginosa Isolated From Patients With Ventilator Associated Nosocomial Infection

“…This is an important phenomenon in the creation and distribution of new resistance cassettes. Importance of association of multidrug resistance and presence of integron plays an important role in the development of multiple resistance (15)(16)(17). A shift in the distribution of ESBL-producing strains has recently occurred with reports from North America, Europe, South America, Africa, and Asia, with a dramatic increase of CTX, TEM, and SHV variants (18-21).…”