Multidrug resistant pathogenic bacterial biofilm inhibition by Lactobacillus plantarum exopolysaccharide

Pradeepa,; Shetty, Akshay D.; Matthews, K. P.; Hegde, Aswathi R.; Akshatha, B.; Mathias, Alvita Betilla; Mutalik, Srinivas; Vidya, S. M.

doi:10.1016/j.bcdf.2016.06.002

Cited by 21 publications

(4 citation statements)

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“…Some exopolysaccharides can perform functions that inhibit or destabilize the biofilms without exhibiting biocidal or bacteriostatic activities [122,124]. Their antibiofilm activity is likely to be mediated by mechanisms other than growth inhibition [125][126][127][128][129][130][131][132][133][134][135][136][137][138][139]. Most antibiofilm polysaccharides act as surfactant molecules that modify the physical characteristics of bacterial cells and abiotic surfaces [122].…”

Section: Antiadhesive Activity Of Bacterial Exopolysaccharidesmentioning

confidence: 99%

“…Equally, evidence suggests that polysaccharides not only modify abiotic surfaces but also alter the physical properties of both Gram-negative and Gram-positive bacterial cell surfaces [122]. Many authors have highlighted a direct interaction between the EPS and the cell surface, leading to a reduction in surface hydrophobicity, cell interactions, and antibiotic resistance patterns [126,128]. Hence, polysaccharides act not only in initial adhesion by weakening cell-surface contacts but also in biofilm maturation by reducing cell-cell interactions [129].…”

Section: Antiadhesive Activity Of Bacterial Exopolysaccharidesmentioning

confidence: 99%

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Biobased Anti-Adhesive Marine Coatings from Polyhydroxyalkanoates and Polysaccharides

et al. 2023

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Due to environmental regulations, antifouling marine coatings must be gradually replaced by biocide-free coatings. Marine organisms weakly adhere to fouling release coatings, presenting a low surface free energy and a high elasticity, so they can be readily removed by the sheer force of water. Currently, these materials are mainly composed of petrochemical polymers, such as silicone or fluoropolymers, with hydrophilic polymers as additives. However, following the ever-increasing environmental concerns, the research on new, alternative, eco-friendly coatings is oriented towards the use of biobased polymers from renewable resources. Two main families have been studied: polyhydroxyalkanoates (PHAs) and polysaccharides. PHAs are produced by bacteria in stressful conditions, while polysaccharides are extracted from plants, animals, or micro-organisms such as bacteria, in which case they are called exopolysaccharides (EPS). Since the use of these polymers is a non-toxic approach to controlling fouling colonization, this review provides an overview of these biobased polymers for their applications in new anti-adhesive marine coatings.

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Section: Antiadhesive Activity Of Bacterial Exopolysaccharidesmentioning

confidence: 99%

Section: Antiadhesive Activity Of Bacterial Exopolysaccharidesmentioning

confidence: 99%

Biobased Anti-Adhesive Marine Coatings from Polyhydroxyalkanoates and Polysaccharides

et al. 2023

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“…The results revealed that selected strains were able to inhibit biofilm formation of B. cereus ATCC14579 and S. salivaris B468. Pradeepa et al (2016) evaluated the antibiofilm activity of the exopolysaccharide (EPS) isolated from fish-derived L. plantarum. The results of this study indicated that EPS exhibited a good antibiofilm activity against biofilm-forming multi-drug resistant bacteria because direct interaction between EPS and pathogenic cell surface led to a reduction in surface hydrophobicity, cell interaction, and antibiotic resistance patterns.…”

Section: An Analysis Of the Influence Of Lactobacillus Acidophilus -Dmentioning

confidence: 99%

Biosurfactant from Lactobacillus sp. as an antibiofilm agent

Kaur¹,

Kaur²

2019

bta

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In the era of antibiotic resistance, probiotics have emerged as potential therapeutic alternatives to antibiotics.The probiotic potential of Lactobacillus is well known, but it is also a promising source of novel pharmaceutical products and is used in various food applications such as functional foods, antibiofilm agents, and antimicrobials. Moreover, the synthesis of various chemicals, pharmaceuticals, organic acids, biosurfactants, and bacteriocins from these bacteria has been previously reported. Because of the increasing occurrence of antibiotic resistance, biofilm infections are remarkably difficult to treat. Furthermore, recent studies have acknowledged the role of biosurfactants released by Lactobacillus sp. in restricting the development of biofilms owing to their well-known activity as a biosurfactant in biomedical and food processing industries. Biosurfactants produced by Lactobacillus sp. have shown antimicrobial activity by interfering with the biofilm formation; therefore, it is important to understand the role of biosurfactants from Lactobacillus sp. as antibiofilm agents. In this review, we attempt to focus on the problems associated with the production of pathogenic biofilms in the biomedical sciences and food industry. Furthermore, the importance of biosurfactants from probiotic Lactobacillus sp. in inhibiting biofilm production has also been discussed.

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“…EPSs isolated from L. plantarum can also reduce biofilm production by inhibiting indole production associated with quorum sensing, as shown in Escherichia coli [18]. Shetty et al [19] also found that different EPS contents of L. plantarum can alter the outer membranes of multidrug-resistant bacteria, resulting in a higher antibiotic susceptibility in biofilms. However, there is no evidence in the literature to support the efficacy of L. plantarum EPSs, and particularly its antibiofilm activity, against the oral opportunists or pathogens investigated in this study.…”

Section: Introductionmentioning

confidence: 98%

Analysis of Chemical Structure and Antibiofilm Properties of Exopolysaccharides from Lactiplantibacillus plantarum EIR/IF-1 Postbiotics

et al. 2022

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Previous studies have indicated that the exopolysaccharides of lactic acid bacteria exhibit antibiofilm activity against non-oral bacteria by preventing their initial adhesion to surfaces and by downregulating the expression of genes responsible for their biofilm formation. The aims of this study were to (1) characterize the exopolysaccharides (EPSs) of Lactobacillus plantarum EIR/IF-1 postbiotics, (2) test their antibiofilm effect on dual biofilms, and (3) evaluate their bacterial auto-aggregation, co-aggregation, and hydrocarbon-binding inhibitory activity. The EPSs were characterized by FTIR, HPLC, and thermogravimetric analysis. Bacterial auto- and co-aggregation were tested by Kolenbrander’s method and hydrocarbon binding was tested by Rosenberg’s method. Dual biofilms were formed by culturing Fusobacterium nucleatum ATCC 25586 with one of the following bacteria: Prevotella denticola ATCC 33185, P. denticola AHN 33266, Porphyromonas gingivalis ATCC 33277, P. gingivalis AHN 24155, and Filifactor alocis ATCC 35896. The EPSs contained fractions with different molecular weights (51 and 841 kDa) and monosaccharides of glucose, galactose, and fructose. The EPSs showed antibiofilm activity in all the biofilm models tested. The EPSs may have inhibited bacterial aggregation and binding to hydrocarbons by reducing bacterial hydrophobicity. In conclusion, the EPSs of L. plantarum EIR/IF-1, which consists of two major fractions, exhibited antibiofilm activity against oral bacteria, which can be explained by the inhibitory effect of EPSs on the auto-aggregation and co-aggregation of bacteria and their binding to hydrocarbons.

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Multidrug resistant pathogenic bacterial biofilm inhibition by Lactobacillus plantarum exopolysaccharide

Cited by 21 publications

References 50 publications

Biobased Anti-Adhesive Marine Coatings from Polyhydroxyalkanoates and Polysaccharides

Biobased Anti-Adhesive Marine Coatings from Polyhydroxyalkanoates and Polysaccharides

Biosurfactant from Lactobacillus sp. as an antibiofilm agent

Analysis of Chemical Structure and Antibiofilm Properties of Exopolysaccharides from Lactiplantibacillus plantarum EIR/IF-1 Postbiotics

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