Multidrug-resistant Salmonella enterica serotype Senftenberg isolates producing CTX-M β-lactamases from Constantine, Algeria

Naas, Thierry; Lezzar, Abdesselam; Bentchouala, Chafia; Smati, F; Scheftel, J.M.; Monteil, H.; Nordmann, Patrice

doi:10.1093/jac/dki216

Cited by 29 publications

(20 citation statements)

References 6 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…ESBL-positive Enterobacteriaceae are frequently isolated in hospitals in Algeria, and the overall frequency of ESBL producers at the Mustapha Pacha hospital from January (5), and CTX-M-3 in S. enterica serovar Senftenberg in Algeria (15). The frequency of Enterobacteriaceae producing ESBL in Algeria has not been reported.…”

Section: Sixteen Strains Ofmentioning

confidence: 99%

CTX-M-3 and CTX-M-15 Extended-Spectrum β-Lactamases in Isolates of Escherichia coli from a Hospital in Algiers, Algeria

et al. 2006

View full text Add to dashboard Cite

Sixteen strains of Escherichia coli isolated between January and June 2005 in a hospital in Algiers carry the ISEcp1 element and the TEM and either CTX-M-3 (n ‫؍‬ 3) or CTX-M-15 (n ‫؍‬ 13) ␤-lactamases. Fourteen of the isolates are multidrug resistant. Five isolates from the neonatal ward were indistinguishable by pulsed-field gel electrophoresis.CTX-M-type enzymes are the extended-spectrum ␤-lactamases (ESBL) most commonly produced by Enterobacteriaceae (4), and more than 55 CTX-M-type ␤-lactamases have been described (http://www.lahey.org/studies/webt.htm). Despite the prevalence of ESBL in Enterobacteriaceae, data from Algeria are scarce (although the prevalence has been reported to be 20 to 45%) (18). We investigated the phenotypic and ge-

show abstract

Section: Sixteen Strains Ofmentioning

confidence: 99%

CTX-M-3 and CTX-M-15 Extended-Spectrum β-Lactamases in Isolates of Escherichia coli from a Hospital in Algiers, Algeria

et al. 2006

View full text Add to dashboard Cite

show abstract

“…Extended-spectrum ␤-lactamases (ESBLs) of the CTX-M type have been reported increasingly in gram-negative rods, mostly in Escherichia coli (4,5,9,13,17,24,31,42). The first CTX-M ␤-lactamase (CTX-M-1/MEN-1) was characterized in E. coli strains isolated from German and Italian patients (2,3).…”

mentioning

confidence: 99%

Identification of CTX-M-Type Extended-Spectrum-β-Lactamase Genes Using Real-Time PCR and Pyrosequencing

Naas

Oxacelay

Nordmann

2007

Antimicrob Agents Chemother

Self Cite

View full text Add to dashboard Cite

CTX-M extended-spectrum ␤-lactamases (ESBLs) are increasingly prevalent worldwide among Escherichia coli bacteria, mostly in community-acquired urinary tract infections. Finding a fast and reliable technique for identification of CTX-M enzymes is becoming a challenge for the microbiology laboratory. A fast real-time PCR amplification technique, using degenerated primers specific for all the bla CTX-M alleles, coupled to real-time pyrosequencing was developed. The five CTX-M groups were unambiguously identified by pyrosequencing a 13-bp DNA region. Further sequencing of an additional 16-bp region allowed further division into subgroups. Phylogenetic trees constructed with the entire bla CTX-M genes and with both pyrosequenced regions (29 bp) gave similar results, suggesting that this technique, termed the real-time detection and sequencing method, has a powerful discriminatory ability. This high-throughput technique has been evaluated by screening 48 ESBLproducing E. coli isolates recovered from the Bicêtre hospital (France) in 2004. Forty-four of these strains were CTX-M positive by real-time PCR detection and direct pyrosequencing of the PCR products, which identified CTX-M-15 as the main CTX-M-type ␤-lactamase. Pulsed-field gel electrophoresis analysis of these strains revealed that several clones, of which one CTX-M-15-positive clone was predominant (60%), were identified both in nosocomial and in community-acquired isolates. The combination of real-time PCR with pyrosequencing represents a powerful tool for epidemiological studies of CTX-M producers. This assay has the potential to be used in a diagnostic laboratory since up to 96 bacterial isolates may be screened in less than 3 h.

show abstract

“…Recent studies have shown the presence of these enzymes in African countries (2,13,14,24,31,37,39). In Tunisia, the first identified CTX-M-producing strain (CTX-M-3), Salmonella enterica serovar Wien, was recovered in Tunis in 2001 (1).…”

mentioning

confidence: 99%

Emergence and Outbreaks of CTX-M β-Lactamase-Producing Escherichia coli and Klebsiella pneumoniae Strains in a Tunisian Hospital

et al. 2006

View full text Add to dashboard Cite

show abstract

Multidrug-resistant Salmonella enterica serotype Senftenberg isolates producing CTX-M β-lactamases from Constantine, Algeria

Cited by 29 publications

References 6 publications

CTX-M-3 and CTX-M-15 Extended-Spectrum β-Lactamases in Isolates of Escherichia coli from a Hospital in Algiers, Algeria

CTX-M-3 and CTX-M-15 Extended-Spectrum β-Lactamases in Isolates of Escherichia coli from a Hospital in Algiers, Algeria

Identification of CTX-M-Type Extended-Spectrum-β-Lactamase Genes Using Real-Time PCR and Pyrosequencing

Emergence and Outbreaks of CTX-M β-Lactamase-Producing Escherichia coli and Klebsiella pneumoniae Strains in a Tunisian Hospital

Contact Info

Product

Resources

About